Primer and probe combination for RF-RAA detection of duck circovirus and application thereof

Abstract

The invention relates to the technical field of virus detection, and particularly discloses a primer and probe combination for RF-RAA detection of duck circovirus and application thereof. In the primers and the probe for detecting the duck circovirus, the sequence of an upstream primer is shown as SEQ ID NO: 1, the sequence of a downstream primer is shown as SEQ ID NO: 2, and the sequence of the probe is shown as SEQ ID NO: 3. Specific primers and probes are designed according to a conserved motif of DuCV, an RF-RAA method applied to detection of DuCV is established, accumulation of amplification products is positively correlated with the intensity of fluorescence signals, an obvious result can be observed through an instrument after the reaction is performed for 15 min at 41 DEG C, and the method is high in specificity, high in sensitivity, high in accuracy, capable of achieving the lowest detection limit of 1 copy / microliter and capable of being applied to detection of DuCV. Reliable guarantee is provided for early clinical detection and epidemiological investigation of the duck circovirus, and positive significance is achieved on prevention and control of epidemic diseases.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a combination of primers and probes for detecting duck circovirus by RF-RAA and an application thereof. Background technique [0002] Duck circovirus disease (DuCVD) is an infectious disease caused by duck circovirus (Duckcircovirus, DuCV), which can infect ducks of all ages and breeds. DuCV was first discovered in 2003, and then cases of infection were reported successively in many countries around the world. DuCV can attack the immune system of ducks, causing immunosuppression, leading to secondary infection or multiple infection. Affected ducks manifested as hair loss, loss of appetite, growth retardation, severe cases and even dyspnea, anemia, decreased production performance and even death. [0003] The symptoms of DuCVD are similar to those of some bacterial and fungal infections and some nutritional diseases, and it is difficult to make accurate clinical judgment...

AI Technical Summary

Problems solved by technology

[0004] In view of the time-consuming, poor specificity, low sensitivity and the need for special instruments in the existing methods for detecting duck circovirus, the present invention provides a combination of primers and probes for RF-RAA detection of duck circovirus and its application, The primers and probes for detecting duck circovirus can be used to detect duck circovirus quickly and accurately at a constant temperature of 37-41°C, and it has the advantages of simple operation, good specificity, and high sensitivity, and has positive significance for the prevention and control of DuCVD

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