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Injection filler containing deproteinized bone and preparation method thereof

A filler and protein bone technology, which is applied in the fields of medical cosmetology and plastic surgery, can solve the problems affecting the effectiveness of fillers, uneven product components, and inability to synergize, etc., to achieve prolonged retention time, excellent hydrophilic properties and biocompatibility Sexuality, the effect of promoting growth and differentiation

Active Publication Date: 2021-12-28
西安德诺海思医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method relies on the reaction system, which is prone to the problem of uneven product composition. At the same time, the solid microspheres formed have certain hydrophobicity, which causes the risk of foreign body reaction in the body of the filler.
At the same time, in the choice of gel matrix, or simply choose the polysaccharide component, so that the synergistic effect of promoting autologous collagen regeneration cannot be achieved together with the microsphere component; or choose the composite component of polysaccharide and collagen, but the retention time in the body is too short ; thereby affecting the effectiveness of the filler

Method used

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  • Injection filler containing deproteinized bone and preparation method thereof
  • Injection filler containing deproteinized bone and preparation method thereof
  • Injection filler containing deproteinized bone and preparation method thereof

Examples

Experimental program
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Effect test

example 1

[0050] 1) Preparation of gel phase

[0051] Get the cross-linking agent BDDE and dissolve it in 1M NaOH solution according to the final concentration of 2wt%, and then add sodium hyaluronate (final concentration 15wt%) with a molecular weight of 120w-140w and recombinant collagen (final concentration) with a molecular weight of about 60K in the solution Concentration 10wt%), stir evenly, cross-link at 50°C for 4 hours. Cut the cross-linked gel into small pieces and wash it with pure water to remove reagent residues, then dilute to the final concentration of sodium hyaluronate at 20 mg / mL, the final concentration of lidocaine hydrochloride is 2mg / mL; make the final required gel (as the gel phase of the injection filler) by sieving or homogenizing.

[0052] 2) Preparation of bone pellets

[0053] Animal (such as cattle, pigs) bone is cut into pieces, washed with pure water to no bone marrow and blood, crushed into particles with a particle diameter of 2-6mm, and then mixed wi...

example 2

[0063] 1) Preparation of gel phase

[0064] Take the cross-linking agent dimethyl sulfoxide and dissolve it in 0.25M KOH solution according to the final concentration of 4.5wt%, and then add sodium hyaluronate with a molecular weight of 220w-240w (final concentration 8wt%) and a molecular weight of about 90K to the solution. The recombinant collagen (final concentration 16wt%) was stirred evenly, and cross-linked at 40° C. for 16 hours. Cut the cross-linked gel into small pieces and wash it with pure water to remove reagent residues, then dilute to the final concentration of sodium hyaluronate at 10 mg / mL, the final concentration of benzocaine is 4mg / mL; make the final required gel (as the gel phase of the injection filler) by sieving or homogenizing.

[0065] 2) Preparation of deproteinized bone particles

[0066] Animal (such as cattle, pigs) bone is cut into pieces, washed with pure water until there is no bone marrow and blood, crushed into particles with a particle siz...

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Abstract

The invention discloses an injection filler containing deproteinized bone and a preparation method thereof. The filling is formed by mixing recombinant collagen-deproteinized bone composite microspheres and gel containing cross-linked sodium hyaluronate and recombinant collagen, wherein a microsphere phase in the filling is formed by performing virus killing, degreasing, decellularization and deproteinization treatment on animal-derived bone tissue particles, crushing and passivating to form particles which are free of edges and corners and irregular in appearance, and performing emulsion cross-linking on the particles and the collagen to prepare the microsphere phase; the gel phase is prepared by mixing sodium hyaluronate and recombinant collagen into a phosphate buffer solution containing or not containing an anesthetic after cross-linking the sodium hyaluronate and the recombinant collagen. According to the invention, by adopting a natural bone material, the deproteinized bone microparticle structure in the composite microsphere cross-linked recombinant collagen shell is endowed with excellent hydrophilicity and compatibility, and meanwhile, the injection filler formed by mixing a microsphere phase and a gel phase can play a coordinating role in supporting collagen regeneration of an organism, and provide a long-acting and safe supporting effect for the skin.

Description

technical field [0001] The invention belongs to the technical field of medical cosmetology and plastic surgery, and in particular relates to an injection filler containing deproteinized bone and a preparation method thereof. Background technique [0002] In order to achieve the effect of facial rejuvenation and aesthetics, the use of soft tissue fillers has received more and more attention, and higher requirements have been put forward for the performance and use of soft tissue fillers: good biocompatibility, high The safety and longer-lasting filling effect. At present, the most commonly used soft tissue filler is sodium hyaluronate filler, which can significantly prolong its retention time in the body after cross-linking treatment, but it still cannot achieve long-term filling effect, and at the same time, it and its degradation product polysaccharides do not have the ability to promote plant growth. The role of endogenous collagen regeneration at the site of injection; a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/44A61L27/36A61L27/50A61L27/52
CPCA61L27/48A61L27/446A61L27/3608A61L27/52A61L27/3691A61L27/50A61L2400/06A61L2300/622A61L2430/34A61L2300/252A61L2300/30C08L89/00C08L5/08
Inventor 张立娟
Owner 西安德诺海思医疗科技有限公司
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