IncRNA marker related to bacterial diarrhea resistance of piglets and application of IncRNA marker

A bacterial, lncrna-fut3-as1 technology, applied in the direction of DNA / RNA fragments, antibacterial drugs, recombinant DNA technology, etc., can solve the problem of less regulation of diarrhea in weaned piglets

Inactive Publication Date: 2021-12-24
YANGZHOU UNIV
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Problems solved by technology

At present, there are few reports on the regulatory effect of lncRNA on diarrhea in weaned piglets

Method used

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  • IncRNA marker related to bacterial diarrhea resistance of piglets and application of IncRNA marker
  • IncRNA marker related to bacterial diarrhea resistance of piglets and application of IncRNA marker
  • IncRNA marker related to bacterial diarrhea resistance of piglets and application of IncRNA marker

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Embodiment Construction

[0040] 1.1 Test material

[0041] The Sutai pigs in the experiment came from the Sutai pig Escherichia coli F18 sensitive and resistant resource groups (Suzhou Sutai Pig Breeding Center) established earlier by the research group. The 35-day-old Sutai weaned piglets from 5 families were selected and combined Receptor binding test and V-type secretion system display functional adhesin test methods, and finally strictly screened out 5 weaned piglets of F18 Escherichia coli sensitive and resistant [136] . Meishan pigs come from Meishan Pig Breeding Co., Ltd. (Kunshan City, Jiangsu Province). Through the comprehensive analysis of post-challenge phenotype and in vitro adhesion test, 5 Meishan pigs were confirmed to be F18 Escherichia coli sensitive and resistant. [137] . The piglets were kept in the same environment, were of similar weight and size, and were in good health. Duodenal tissue samples of piglets were collected after slaughter, stored in liquid nitrogen on site, and t...

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Abstract

The invention relates to an IncRNA marker related to bacterial diarrhea resistance of piglets and application of the IncRNA marker. According to the IncRNA marker and the application thereof, a key candidate lncRNA-FUT3-AS1 for Escherichia coli F18 infection regulation is screened through omics sequencing, the sequence is SEQ ID NO: 1, and on the basis, deep analysis is carried out around the function and regulation mechanism of the FUT3-AS1 in the research; firstly, qPCR and Northern blot technologies are used for detecting the expression difference condition of the FUT3-AS1 in duodenums of F18 Escherichia coli sensitive and resistant weaned piglets of Meishan pigs and Sutai pigs, lncRNA-FUT3-AS1-silenced pig small intestine epithelial cells are successfully established, and the interference efficiency reaches 68%; and a series of detection means such as bacterial counting, Gram's staining, scanning electron microscopy and indirect immunofluorescence find that the adhesion capability of F18 Escherichia coli to IPEC-J2 cells can be remarkably lowered by silencing lncRNA-FUT3-AS1. Therefore, the lncRNA-FUT3-AS1 plays an important resistance regulation function in a process of resisting F18 Escherichia coli infection of the weaned piglets, the adhesion ability of the small intestine epithelial cells to the F18 Escherichia coli is lowered by down-regulating an expression level of the lncRNA-FUT3-AS1, and the resistance ability of the piglets to the F18 Escherichia coli infection is favorably improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an lncRNA marker lncRNA-FUT3-AS1 related to piglet bacterial diarrhea, and its application as a target in improving pigs' resistance to Escherichia coli. Background technique [0002] Escherichia coli F18 is one of the important pathogenic bacteria that cause bacterial diarrhea in weaned piglets in today's pig industry. Symptoms such as edema and diarrhea occur. Therefore, whether weaned piglets can resist the infection of E. coli F18 mainly depends on whether the E. coli F18 receptor of the small intestinal mucosal epithelial cells expresses. Foreign studies have found that type 1 H antigen, which participates in the composition of ABH blood group antigen, is the smallest epitope of the adhesion receptor of F18 Escherichia coli, mainly catalyzed by α-(1,2) fucosyltransferase (FUT2 enzyme), and FUT2 enzyme The functional activity of FUT1 is jointly regulated by the FUT1 and FUT2 ge...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12N15/113A61K45/00A61P1/12A61P31/04
CPCC12Q1/6888C12Q1/686C12N15/113A61K45/00A61P1/12A61P31/04C12Q2600/124C12Q2600/178C12Q2600/158
Inventor 包文斌范海瑞吴正常任战士吴圣龙殷宗俊王海飞
Owner YANGZHOU UNIV
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