Fermentation culture medium for producing riboflavin and fermentation method

Abstract

The invention discloses a fermentation culture medium for producing riboflavin and a fermentation method. The fermentation culture medium comprises the following components: 20-30 g / L of glucose, 10-20 g / L of yeast powder, 10-20 g / L of soybean cake powder, 10-20 g / L of corn steep liquor, 2-5 g / L of sodium citrate, 3-6 g / L of dipotassium phosphate, 1-3 g / L of ammonium sulfate, 0.5-1.5 g / L of sodium nitrate, 100-200 mL of glutamine mother liquor and the balance of water. The fermentation method comprises the following steps: (1) preparing the fermentation culture medium; (2) performing sterilizing; (3) performing inoculating with a bacillus subtilis seed solution; and (4) performing fermentation culture. The fermentation culture medium has the beneficial effects that the sodium citrate is added to inhibit a glycolysis pathway and increase the carbon flux of an HMP pathway; and glutamine is used as a synthesis precursor of guanosine triphosphate (GTP) and also directly promotes riboflavin generation. According to the fermentation method disclosed by the invention, the production level and the conversion rate of vitamin B2 are greatly improved, the fermentation cost is reduced, and the fermentation method disclosed by the invention is simple and easy to implement.

Description

Technical field: [0001] The patent of the invention belongs to the technical field of biological fermentation engineering, and specifically relates to a fermentation medium and a fermentation method for producing riboflavin. Background technique: [0002] Riboflavin, also known as vitamin B2, is one of the 13 vitamins necessary for the human body. It is widely distributed in nature and is a member of the B vitamins. Riboflavin is a coenzyme component of flavin enzymes. It mainly exists in the form of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) in organisms, and participates in various enzymes in the form of coenzymes or prosthetic groups. An enzyme system reaction. Coenzymes as flavoproteins play an important role in respiration and biological oxidation, directly participate in the biological oxidation of carbohydrates, proteins, and fats, and are essential nutrients for maintaining normal metabolism and physiological functions of the body. At the sam...

AI Technical Summary

Problems solved by technology

Chemical synthesis and semi-microbial fermentation synthesis were developed earlier, and the production process is relatively mature, but their use has been restricted due to serious environmental pollution and complex operating procedures.

In the existing microbial fermentation process, some enterprises use yeast fermentation to produce riboflavin, but the overall cycle is long; most enterprises use Bacillus for fermentation, but due to the complex metabolic pathway of riboflavin, the metabolic process is subject to nuclear The flavin operon, purine de novo synthesis pathway and pentose phosphate (HMP) pathway are coordinated and regulated. At the same time, the increase of ammonium ion concentration in the later stage of fermentation will also shift the metabolic flow, affect the production of riboflavin, and lead to the actual yield and conversion rate of the product Relatively low, high production costs

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