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Multifunctional polymer diagnosis and treatment integrated nano probe, preparation method and application thereof

A nano-probe and polymer technology, applied in the fields of nano-materials and biomedical engineering, can solve the problems that a single imaging method cannot meet the needs of early diagnosis of tumors and their metastasis, missed diagnosis and misdiagnosis of tumors and their metastasis, and achieve good optical imaging. Effect of heat therapy, effect of good colloidal stability

Pending Publication Date: 2021-12-21
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a multifunctional polymer nano-probe for diagnosis and treatment, its preparation method and application, so as to solve the problem in the prior art that the use of a single imaging method cannot meet the needs of early diagnosis of tumors and their metastasis, which may lead to tumor Misdiagnosis, misjudgment and technical problems of misdiagnosis

Method used

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  • Multifunctional polymer diagnosis and treatment integrated nano probe, preparation method and application thereof
  • Multifunctional polymer diagnosis and treatment integrated nano probe, preparation method and application thereof
  • Multifunctional polymer diagnosis and treatment integrated nano probe, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] FE 3 O 4 & IR-1061 @ PLGA Nano Probe (FiP NPS) Preparation:

[0028] Single emulsification - solvent volatile method. In the specific experiment, first take 25ml (0.25 mg, 10 mg / ml) Fe with pipette. 3 O 4 (Chloro) and 80 mL (0.2 mg, 2.5 mg / ml) IR-1061 (DCM oil phase), each added to 1 mL EP tube, and naturally volatilized in the wind compartment at room temperature. Next, 10 mg of PLGA was dissolved in 500 mL DCM and sufficiently dissolved in an ultrasonic cleaning device for 2 minutes, and it was sufficiently dissolved to form an oil phase (O); 50 mg PVA (0.5%) was dissolved in 10 g of ultra-pure water, It is sufficiently dissolved to form aqueous phase (W) in ultrasound cleaning. Then in the previously volatile Fe 3 O 4 Each of the IR-1061 EP is added to 200 ml DCM, and the ultrasound was dispersed in the ultrasound cleaner for 2 minutes; after the pipette will use the pipe gun 200 ml Fe 3 O 4 (DCM oil phase) Add 200 mL IR-1061 (DCM oil phase), and dispersed for 2 minu...

Embodiment 2

[0030] Example 2 Characterization of FIP NPS:

[0031]1) FIP NPS electron microscope preparation and photo, particle size measurement

[0032] The FIP NPS prepared for Example 1 was diluted to a suitable concentration for electron microscopy, electron microscopy figure 1 (a) shows that the particle size statistics figure 1 (b) indicates that its average size is 98.4 ± 28.5 nm.

[0033] 2) FIP NPS hydraulic size, potential and stability test

[0034] a. Take a proper amount of FIP NPS solution, dilute to a suitable concentration, move to a cuvette, and measure the particle size and potential of the same sample at different time points to compare whether there is any change.

[0035] b. In order to explore the stability of FIP NPS in the organism, the extraction amount of FIP NPS is added, and the particle size and potential of the same sample is measured at different time points to observe whether it has changed. .

[0036] like figure 2 (a) As shown, the fluid mechanical dimension...

Embodiment 3

[0041] Example 3 Cytotoxic Experiment

[0042] Different concentrations of FIP NPs were incubated with mouse breast cancer 4T1 cells for 24 h, and via a thiazole Blue Composite (MTT) was subjected to in vitro cytotoxicity.

[0043] Figure 4 The cell survival rate for 4T1 breast cancer cells and FIP NPS was incubated with FIP NPS, which can be seen from the figure that the concentration of 4T1 cell survival and FIP NPS depends, the higher the concentration of the FIP NPS, and the lower the cell survival rate. However, even if the FIP NPS concentration is 24 mg / ml, the survival rate of 4T1 cells is also over 70%.

[0044] Experimental results show that higher concentration FIP NPS has no obvious toxic effect on 4T1 cells.

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Abstract

The invention discloses a multifunctional polymer diagnosis and treatment integrated nano probe, a preparation method and application of the nano probe in preparation of a medicine for diagnosing and treating lymphatic metastasis tumor of breast cancer. Wherein the nano probe is a nano microsphere which is obtained by coating Fe3O4 nano particles and a near-infrared II region fluorescent dye IR-1061 with PLGA organic molecule. The multifunctional polymer diagnosis and treatment integrated nano probe disclosed by the invention has good colloidal stability, optical property and in-vitro photothermal effect, and has no obvious cytotoxicity; a good MRI / OI / PAI / SPECT multi-mode imaging effect is achieved, and accurate positioning and tracing of living breast cancer lymph node metastasis are achieved; and the nano probe has a good photo-thermal treatment effect and can be used for photo-thermal treatment of patients with breast cancer lymph node metastasis.

Description

Technical field [0001] The present invention relates to a multifunctional polymer diagnosis and treatment integrated nano probe, preparation method and application, belonging to the field of nanomaterials and biomedical engineering. Background technique [0002] It is now clinically used to determine the common non-invasive imaging examination methods for breast cancer lymph node metastasis mainly include ultrasound, molybdenum target X-ray slice, CT and MRI examination, etc., but most of these examinations are based on anatomical morphological structural changes to observe the lesion; Although there is an advantage, the shortcomings of its facilities have led to limitations in their application; therefore the diagnosis of early metastasis is not ideal, and it is easy to cause misdiagnosis and missed diagnosis. It can be seen that it is especially important to seek an imaging examination method that explicitly diagnoses breast cancer lymph node metastasis. [0003] There are curr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K49/18A61K49/12A61K49/04A61K49/00A61K41/00A61P35/04
CPCA61K49/225A61K49/1857A61K49/12A61K49/04A61K49/0093A61K49/0054A61K49/0021A61K49/0002A61K41/0052A61P35/04
Inventor 蔡武范国华徐亮
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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