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Thin-layer identification method for allium macrostemon test sample

A technology for thin-layer identification and test items, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of less chromatographic information, blurred color spots, poor separation effect, etc., and achieve simple inspection process, good separation, Guaranteed curative effect

Active Publication Date: 2021-12-07
BEIJING KANGRENTANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention is to overcome the defects of blurred color spots, poor separation effect, and little chromatographic information in the identification of thin-layer chromatography in the prior art; A thin-layer identification method for allium scallions, which has clear stains and is more friendly to operators

Method used

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  • Thin-layer identification method for allium macrostemon test sample
  • Thin-layer identification method for allium macrostemon test sample
  • Thin-layer identification method for allium macrostemon test sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] TLC Xiebai method of the test procedure is as follows:

[0047] 1. Preparation for the test solution:

[0048] Preparation of test solution: Take scallions white water extract 0.5g, grind fine, add water 30ml, in 3 ml of hydrochloric acid was heated at reflux for 1 hour, allowed to cool, extracted twice with ethyl acetate shaking, each 30ml, combined ethyl acetate solution was concentrated to 5ml, as the test solution.

[0049] Preparation of the test solution for: taking medicine Xiebai 1.5g, finely divided, water 50ml, 3ml of hydrochloric acid, heated at reflux for 1 hour, allowed to cool, extracted twice with ethyl acetate shaking, each 30ml, combined ethyl acetate solution, and concentrated to 5ml, as the test solution.

[0050] Preparation of drug solution control: Take Xiebai reference drug 1.5g, finely divided, water 50ml, 3ml of hydrochloric acid, heated at reflux for 1 hour, allowed to cool, extracted twice with ethyl acetate shaking, each 30ml, combined ethyl acetat...

Embodiment 2

[0055] The present embodiment is a difference in that Example, the present invention is employed in the test solution employed in Example 1 as a lot of scallions 200618-725800-01,200529-724400-02,200610-713800-03 same whitewater extract manner prepared, and the test solution and control the amount of different kind of drug solution, specifically configured to: sample volume point respectively 1μl, 2μl, 3μl, results are image 3 Indicated.

[0056] Should image 3 , The numbers 1-3 sequentially correspond 1μl, 2μl, 3μl of shallot extract 200618-725800-01 Whitewater, numbered 4-6 followed by 1μl, 2μl, 3μl of shallot extract 200618-725800-02 Whitewater, No. 7-9 sequentially correspond [mu] l, shallot white water 2μl, 3μl of extract 200618-725800-03, No. 10-12 sequentially correspond 1μl, 2μl, 3μl of Xiebai control medicine.

[0057] By examining the amount of spotting, scallion white water extract, Xiebai control medicine was spotted 2 l respectively, when [mu] l, TLC spots were clear,...

Embodiment 3

[0059] TLC embodiment of the present embodiment in Example 1 were verified durability, comprises:

[0060] 1, different temperature and humidity Investigation

[0061] Prepared in Example 1 using the obtained test solution (shallot extract Whitewater: 200618-725800-01,200529-724400-02,200610-713800-03), the reference drug solution, respectively, at normal temperature and low humidity (T: 29 ℃ , RH: 10%), low humidity (T: 4 ℃, RH: 60%), high temperature and humidity (T: 29 ℃, RH: 80%) conditions, using the same method as in Example 1 to start out , drying, UV light (365nm) under review, see results Figure 4-6 . Should Figure 4-6 Numbered 1-3 correspond shallot extract 200618-725800-01 Whitewater, shallot extract 200529-724400-02 white water, white water extract 200610-713800-03 shallot, scallion white No. 4 corresponding to the reference drug.

[0062] 2, effects of different lamina

[0063] Using the test solution (shallot extract Whitewater: 200618-725800-01,200529-724400-02,2006...

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Abstract

The invention provides a thin-layer identification method of an allium macrostemon test sample, which comprises the following steps: preparing an allium macrostemon medicinal material, an aqueous extract or formula granules into a test sample solution; and dripping the test solution on a silica gel G thin-layer plate, performing developing by using petroleum ether-ethyl acetate-dichloromethane-formic acid as a developing solvent according to a volume ratio of (5-6): (3.5-4): (1-1.2): (0.8-1), taking out the silica gel G thin-layer plate, performing airing, and performing inspecting by using an ultraviolet lamp. The method has the advantages that more thin-layer chromatography information spots can be obtained compared with that in pharmacopoeia, and thin-layer information is rich; in addition, the method has the advantages that displayed spots are clear, the separation degree is good, and the repeatability is good.

Description

Technical field [0001] The present invention relates to the field of thin layer identification, and more particularly to a thin layer identification method of a whitening supply. Background technique [0002] White is a lilies, a small root, a small root, or a dry bulbs of allium chinenseg.don.薤 白 is the traditional Chinese medicine, tempered, taste, bitter. At home, lung, stomach, large intestine. With anti-tumor, antioxidation, inhibiting platelet agglomeration, lipid-lowering, bacteriobacteria and hyphen asthma, can be used to treat diseases such as coronary heart disease and angina, bronchial asthma, high blood fat and myocardial ischemia. The white herbal medicine has the advantages of low toxicity, wide security, rich drug source, low prices, so that the research and development of 薤 薤 薤 药 制 制 has a good prospect. [0003] The white chemical composition is complex, there is a variety of ingredients such as steroid saponins, volatile oil, nitrogen-containing compound, acidic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 张志强李国鹏李蕊付静高扬
Owner BEIJING KANGRENTANG PHARMA
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