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Extraction method and application of paulownia bark total flavonoids in paulownia

An extraction method and a technology of total flavonoids, applied in the field of research and development of anti-inflammatory drugs, can solve the problems of unclear anti-inflammatory mechanism of Paulownia, and achieve good efficacy and anti-inflammatory effects

Inactive Publication Date: 2021-12-07
HUANGHE S & T COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the current problem that the anti-inflammatory mechanism of Paulownia is not clear, the present invention provides a method for extracting total flavonoids from inner paulownia bark and its application

Method used

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  • Extraction method and application of paulownia bark total flavonoids in paulownia
  • Extraction method and application of paulownia bark total flavonoids in paulownia
  • Extraction method and application of paulownia bark total flavonoids in paulownia

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Embodiment 1

[0024] Embodiment 1: The present embodiment provides a kind of extraction method of total flavonoids of paulownia inner paulownia bark, the method comprises the following steps:

[0025] S1, drying the paulownia inner paulownia skin, pulverizing, and sieving the paulownia inner paulownia skin powder;

[0026] S2. Add petroleum ether to the paulownia paulownia skin powder for soaking, use 70% ethanol solution to reflux and extract twice at 60° C., extract for 1 hour to obtain an extract, and let stand;

[0027] S3, after separating the cooled supernatant from the raffinate, they are mixed in a rotor evaporator, filtered and concentrated;

[0028] S4, carry out chromatographic separation with macroporous adsorbent resin, then elute with 70% ethanol, concentrate the eluted fraction in vacuum at 40°C, and dry in vacuum at 60°C to obtain the finished product, according to the rutin standard curve formula y =7.515 x +0.2369, the calculated yield of total flavonoids in Paulownia ba...

Embodiment 2

[0029] Example 2: Determination of cytotoxicity and anti-inflammatory effect of total flavonoids of paulownia inner paulownia

[0030] 1. Cell culture and cytotoxicity assay

[0031] The Raw264.7 cell line is a mouse macrophage cell line purchased from the Shanghai Institute of Cells, Chinese Academy of Sciences. Cells were cultured in DMEM medium containing 15% FBS, 100 U / mL penicillin and 100 U / mL streptomycin at 37ºC, 5% CO 2 After 48 hours of subculture, they were subcultured for later use.

[0032] The CCK-8 assay was used to determine the cytotoxicity of Pseudomonas elongatus flavonoids in RAW 264.7 macrophages. Macrophages were grown in 96-well plates (2×104 cells / well), and then the cells were treated with different concentrations of total flavonoids of Paulownia bark (12.5 μg / mL, 25 μg / mL, 50 μg / mL and 100 μg / mL) for 24 hours . After adding 10% CCK-8 working solution, the macrophages were incubated for another 1 hour. Absorbance was determined at 450 nm using a m...

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Abstract

The invention relates to the technical field of paulownia research, in particular to an extraction method and application of paulownia bark total flavonoids in paulownia. According to the invention, the 70% ethanol solution is used for extracting paulownia bark powder, and the prepared paulownia bark total flavonoids have optimal anti-inflammatory activity, can effectively inhibit the expression of proinflammatory factors, are in a dose-dependent manner, and have excellent anti-inflammatory effect.

Description

technical field [0001] The invention belongs to the technical field of research and development of anti-inflammatory drugs, and in particular relates to a method for extracting total flavonoids from inner paulownia paulownia bark and an application thereof. Background technique [0002] Inflammatory response is a complex physiological process triggered by harmful stimuli, such as inflammatory injury, which is a defense response of the body to stimuli. The body's immune system monitors and eliminates this harmful stimulus and heals the damage. Several types of immune cells maintain homeostasis by regulating the levels of inflammatory mediators. Macrophages are the most important inflammatory regulatory cells. They have three major functions in inflammatory response, namely antigen presentation, phagocytosis and immune regulation, and inhibit various inflammatory cytokines and growth factors. Thus, macrophages play a key role in the initiation, maintenance and resolution of ...

Claims

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Application Information

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IPC IPC(8): A61K36/80A61P29/00
CPCA61K36/80A61P29/00A61K2236/35A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61K2236/333
Inventor 李丽妍黄涛兰翀
Owner HUANGHE S & T COLLEGE
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