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Method for simultaneously determining content of indigo blue and indirubin in folium isatidis

A technology of large green leaves and indigo, applied in the field of medicine, can solve the problem of difficult extraction of indigo, and achieve the effect of strong specificity and accurate and reliable display method.

Pending Publication Date: 2021-11-26
HEBEI INST FOR DRUG & MEDICAL DEVICE CONTROL (HEBEI INST FOR COSMETICS CONTROL)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for simultaneously measuring the content of indigo and indirubin in Folium Folium, which can solve the problem that indigo is difficult to extract, and can accurately and conveniently detect the content of indigo and indirubin in Folium Folium simultaneously

Method used

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  • Method for simultaneously determining content of indigo blue and indirubin in folium isatidis
  • Method for simultaneously determining content of indigo blue and indirubin in folium isatidis
  • Method for simultaneously determining content of indigo blue and indirubin in folium isatidis

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Experimental program
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Effect test

preparation example Construction

[0018] Preparation of reference substance solution: Accurately weigh appropriate amount of indigo and indirubin reference substances, add 5% phosphoric acid in N,N-dimethylformamide solution to make a solution containing 30μg per 1ml, and obtain it.

[0019] The preparation of the test solution: Take an appropriate amount of Folium Folium, grind it finely, pass through a No. 3 sieve, take about 0.5g, weigh it accurately, put it in a stoppered Erlenmeyer flask, and accurately add 5% phosphoric acid N,N-dimethyl Dimethyl imide solution 25ml, plugged tightly, weighed, shaken, weighed again, supplemented the lost weight with 5% phosphoric acid N,N-dimethyl imide solution, shook well, filtered, and obtained .

[0020] 1. Verification of extraction conditions

[0021] 1. Effect of phosphoric acid concentration in N,N-dimethylformamide solution

[0022] Take an appropriate amount of samples from the same batch, and use 4% phosphoric acid in N,N-dimethylformamide solution, 5% phosph...

Embodiment

[0058] The chromatographic conditions and system suitability test used C18 column, with methanol-water (70:30) as the mobile phase; the detection wavelength was 289nm. The number of theoretical plates should not be less than 8000 based on the indigo peak.

[0059] Preparation of reference substance solution Take appropriate amount of indigo and indirubin reference substances, weigh them accurately, add 5% phosphoric acid in N,N-dimethylformamide solution to make a solution containing 30μg per 1ml, and get ready.

[0060] Preparation of the test solution Take an appropriate amount of Folium Folium, grind it finely, pass through a No. 3 sieve, take about 0.5g, weigh it accurately, put it in a stoppered conical flask, and accurately add 5% N,N-dimethyl phosphoric acid. 25ml of imide solution, plugged tightly, weighed, shaken for 40 minutes, weighed again, supplemented the lost weight with 5% phosphoric acid N,N-dimethylimide solution, shaked well, filtered, that is have to.

[00...

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Abstract

A method for simultaneously determining content of indigo blue and indirubin in folium isatidis belongs to the technical field of medicines.The folium isatidis is pretreated and extracted by shaking with an organic solvent, and then an extracting solution is inspected by high performance liquid chromatography, and the method comprises the following steps: S1, precisely weighing a proper amount of indigo blue and indirubin reference substances, adding a phosphoric acid-containing N, N-dimethylformamide solution to prepare a reference substance solution; s2, taking the folium isatidis, grinding, sieving with a No.3 sieve, precisely weighing a proper amount of powder, putting into a conical flask with a plug, adding a proper amount of the phosphoric acid-containing N, N-dimethylformamide solution, weighing, shaking, weighing again, complementing the reduced weight with the phosphoric acid-containing N, N-dimethylformamide solution, shaking uniformly, and filtering to obtain the folium isatidis powder; s3, preparing chromatographic conditions: adopting a C18 chromatographic column , 70: 30 methanol-water as a mobile phase, and 289 nm detection wavelength ; and S4, precisely sucking 5 microliters of the reference solution and 5 microliters of the test solution respectively, injecting the solutions into a liquid chromatograph, and determining.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for simultaneously measuring the contents of indigo and indirubin in Folium folium. Background technique [0002] Daqingye is a traditional Chinese medicine in my country. It is the dried leaf of Isatisindigotica Fort., a cruciferous plant. It has the effects of clearing away heat and detoxifying, cooling blood and eliminating spots. The current quality standard of Daqingye is recorded in the first part of the "Chinese Pharmacopoeia" 2020 edition. There are items for the determination of indirubin content, which are determined by extraction with chloroform and determination by high performance liquid chromatography. Other research literatures are also mostly methods for determining the content of indirubin, and the extraction methods also mostly use chloroform extraction, alcohol extraction, etc., which generally have problems such as long time-consuming and cumbersome op...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679
Inventor 王晓蕾刘永利孙慧珠刘晓明王常顺苏建
Owner HEBEI INST FOR DRUG & MEDICAL DEVICE CONTROL (HEBEI INST FOR COSMETICS CONTROL)
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