Device and method for synthesizing diglyceride through multi-liquid-phase system continuous whole-enzyme method

A diglyceride and enzymatic synthesis technology, applied in the direction of sterilization methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of continuous production difficulty, high cost, complex reaction operation, etc., and achieve beneficial The effects of extraction and separation, reduction of material consumption, and simplification of process flow

Active Publication Date: 2021-11-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a continuous full enzymatic synthesis of diacylglycerol in a multi-liquid phase system in view of the problems of high cost, complex reaction operation and difficulty in continuous production in the process of preparing diacylglycerol from existing oil plants. Apparatus and methods for esters

Method used

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  • Device and method for synthesizing diglyceride through multi-liquid-phase system continuous whole-enzyme method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Such as figure 1 As shown, a multi-liquid phase system continuous enzymatic synthesis of diglycerides, including a hydrolysis device and an esterification device, the specific structure is as follows:

[0052] The hydrolysis device includes a device reaction zone 1, a dispersion buffer zone 2, a static zone 3, a first stirrer 4, an automatic potentiometric titrator burette 7, an automatic potentiometric titrator electrode head 13 and a first circulating water heating sleeve 14. Between the device reaction zone 1 and the dispersion buffer zone 2, there is a baffle plate 10 filled with several small holes, and between the device reaction zone 1 and the resting area 3, there is also a baffle plate 12 covered with several small holes. An oil sample inlet 17 and a lower phase solution outlet 18 are arranged at the lower part of the dispersion buffer zone 2 . In the standing area 3, the upper, middle and lower three liquid phase areas are divided. The upper liquid phase area...

Embodiment 2

[0060] The method for continuously synthesizing diglycerides using the device of embodiment 1 may further comprise the steps:

[0061] Step (1): First prepare a 3L aqueous two-phase system, that is, according to the mass fraction of 20% (NH 4 ) 2 SO 4 , 20% PEG600 and 60% water were dissolved and mixed well to prepare in a 3L separatory funnel, and the separatory funnel was placed in an incubator at 37°C for 24 hours. After being clearly separated, the two-phase solution was passed through a 0.45 μm filter membrane to remove impurities to obtain the upper phase PEG600 and the lower phase (NH 4 ) 2 SO 4 solution.

[0062] Step (2): Turn on the first circulating water heat pump 27, add 5g Lipase AY30 enzyme and 10mL [BMIM]BF to 100mL upper phase PEG600 4 , 300mL lower phase Na 2 SO 4 The solution is passed into the static zone 3 under the condition of heating and stirring to make it full of the dispersion buffer 2, and then the PEG600 solution containing enzyme is passed...

Embodiment 3

[0066] The method for continuously synthesizing diglycerides using the device of embodiment 1 may further comprise the steps:

[0067] Step (1): First prepare a 3L aqueous two-phase system, that is, according to the mass fraction of 20% PEG600, 15% Na 2 SO 4 , 65% of the water was dissolved and mixed evenly for preparation in a 3L separatory funnel, and the separatory funnel was placed in an incubator at 37°C for 24 hours. After clear separation, the two-phase solution was passed through a 0.45 μm filter membrane to remove impurities to obtain the upper phase PEG600 and the lower phase Na 2 SO 4 solution.

[0068] Step (2): Turn on the first circulating water heat pump 27, and add 7.5g LipaseTL100L enzyme into 150mL upper phase PEG600. 300mL lower phase Na 2 SO 4 Under the conditions of heating and stirring, pass it into the static zone 3 to make it full of the dispersion buffer 2, and then pass the enzyme-containing PEG600 solution into the reaction zone 1, the stirring...

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Abstract

The invention belongs to the technical field of bioengineering and food, and discloses a device and method for synthesizing diglyceride through a multi-liquid-phase system continuous whole-enzyme method. The device mainly comprises a hydrolysis device and an esterification device, wherein the hydrolysis device is divided into a standing area, a reaction area and a dispersion buffer area from top to bottom; the reaction area and the standing area as well as the reaction area and the dispersion buffer area are separated through baffles, and a plurality of small holes communicated with all the areas are formed in the baffles; the standing area is divided into an upper liquid-phase area, a middle liquid-phase area and a lower liquid-phase area; the upper liquid-phase area is provided with a hydrolysate liquid outlet which is communicated with a sample inlet of the esterification device; fatty acid is directly introduced to synthesize diglyceride by an enzymic method; the middle liquid-phase area is communicated with the reaction area through a return pipe; the lower liquid-phase area is provided with a lower-phase solution sample inlet; an enzyme-containing solution sample inlet is formed in the lower part of the reaction area, and a stirrer is arranged in the reaction area; and an oil sample inlet and a lower-phase solution outlet are formed in the bottom of the dispersion buffer area. The method has the advantages of high repeated utilization rate of raw materials, mild and controllable reaction conditions, continuous production and the like.

Description

technical field [0001] The invention belongs to the field of bioengineering and food technology, relates to the fields of oil industry and oil chemical industry, and particularly relates to a device and method for continuously enzymatically synthesizing diglyceride. Background technique [0002] Diacylglycerol is the esterification product of two hydroxyl groups on glycerol and two fatty acids, and has two isomers, 1,2-DAG and 1,3-DAG. The content of diglyceride in natural animal and vegetable oils is very small. For example, olive oil contains 5.5% diglyceride. Its preparation is mainly completed by oil modification technology, mainly chemical method and enzymatic method. The chemical method has complex process and energy consumption. However, the enzymatic method has attracted much attention because of its high efficiency, mildness, specificity and environmental friendliness. Enzyme methods mainly include oil hydrolysis, direct esterification and glycerol hydrolysis. The...

Claims

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Application Information

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IPC IPC(8): C12M1/40C12M1/34C12M1/02C12M1/00C12P7/64
CPCC12M21/18C12M23/34C12M23/58C12M27/02C12M29/18C12M29/00C12M33/04C12M37/04C12M41/22C12M41/26C12P7/6454Y02P20/54
Inventor 李志刚杨嘉威杨博王永华陈华勇王卫飞
Owner SOUTH CHINA UNIV OF TECH
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