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Method for rapidly and efficiently preparing native tussah silk fibroin protein powder

A technology of tussah silk and plain powder, applied in the field of natural polymers, can solve the problems of unfriendly environment, destroying the natural structure of silk, and cumbersome preparation methods, and achieve the effects of structural integrity preservation, wide application prospects, and low cost

Pending Publication Date: 2021-11-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the disadvantages of being unfriendly to the environment, cumbersome preparation methods, and destroying the natural structure of silk in the existing technology for preparing tussah silk powder, the present invention proposes a method for preparing native tussah silk powder quickly and efficiently
The method of the present invention is specially processed for tussah silkworms, and the original tussah silk fibroin powder with excellent biological properties is obtained through a specific crushing method, which has the characteristics of fast, efficient, and pollution-free, and can retain the biological functions and nano The natural conformational structure (primary structure) of the scale overcomes many problems caused by the existing methods of processing tussah silk fibroin powder

Method used

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  • Method for rapidly and efficiently preparing native tussah silk fibroin protein powder
  • Method for rapidly and efficiently preparing native tussah silk fibroin protein powder
  • Method for rapidly and efficiently preparing native tussah silk fibroin protein powder

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Effect test

Embodiment 1

[0037] In the present embodiment, a method for preparing native tussah silk fibroin powder rapidly and efficiently comprises the following steps in turn:

[0038] (1) Put the cleaned tussah cocoon shells into 0.75% sodium carbonate solution and boil for 0.5 h, repeat this step once, remove the sericin, wash them, place them in an oven at 50° C. and dry them for later use.

[0039] (2) Take 1.0 g of tussah silk fiber degummed in step (1) and put it into a crushing tank, and put 1 stainless steel rotating bead into it, and close the lid tightly. Immerse the crushing tank with the sample in liquid nitrogen for 2 minutes to pre-cool, take it out and quickly fix it on the stainless steel adapter of the crushing instrument. The frequency is set to 30 Hz, and the time is set to 30 s to prepare tussah silk fibroin powder.

[0040](3) move the tussah silk powder obtained in step (2) into a 100-mesh standard sieve, and after sieving and separating, the tussah silk powder with a particl...

Embodiment 2

[0042] (1) Put the cleaned tussah cocoon shells into 0.75% sodium carbonate solution and boil for 0.5 h, repeat this step once, remove the sericin, wash them, place them in an oven at 50° C. and dry them for later use.

[0043] (2) Get 0.2 g of tussah silk fiber degummed in step (1) and put it into a crushing tank, and put 1 stainless steel rotating bead into it, and close the lid tightly.

[0044] Immerse the crushing tank with the sample in liquid nitrogen for 10 minutes to pre-cool, take it out and quickly fix it on the stainless steel adapter of the crushing instrument. The frequency is set to 60Hz, and the time is set to 60s to prepare tussah silk powder (such as image 3 shown).

[0045] (3) The tussah silk fibroin powder obtained in the step (2) is transferred into a 100-mesh standard sieve, and after sieving and separation, the tussah silk fibroin powder with a particle size of 20-50 microns can be prepared.

[0046] (4) Take the tussah silk fibroin powder in step (3...

Embodiment 3

[0050] (1) Weigh 0.5 g of shredded tussah silkworm cocoon shells and put them into a crushing tank, put stainless steel rotating beads into them, and close the lid tightly.

[0051] (2) Immerse the crushing jar with the sample in step (1) in a -80°C refrigerator for 30 minutes, and quickly fix it on the stainless steel adapter of the crushing instrument after taking it out. The frequency is set to 60 Hz, and the time is set to 60 s to prepare tussah cocoon powder.

[0052] (3) Move the tussah powder obtained in step (2) into a 100-mesh standard sieve, and then sieve and separate to prepare tussah cocoon powder with a particle size of about 20-100 microns.

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Abstract

The invention discloses a method for rapidly and efficiently preparing native tussah silk fibroin powder. The method comprises the steps of boiling cleaned tussah cocoon shells in a sodium carbonate solution, repeatedly conducting boiling, removing sericin, and conducting cleaning and drying; and loading the dried tussah silk fibroin fibers into a crushing tank, putting rotating beads into the crushing tank, conducting tight covering with a cover, then immersing the crushing tank into liquid nitrogen or a refrigerator or dry ice for freezing, fixing the fully frozen crushing tank to a stainless steel adapter, starting a freezing crusher for crushing, and conducting screening to prepare the tussah silk fibroin powder with the particle size being micron. The method provided by the invention has the characteristics of rapidness, high efficiency, no pollution and the like, can retain the biological function of the tussah silk fibroin and the natural conformation structure of the nanoscale, overcomes many problems brought by the existing method for processing the tussah silk fibroin powder, and is wide in application.

Description

technical field [0001] The invention relates to a method for preparing silk fibroin protein products, in particular to a method for rapidly and efficiently preparing native tussah silk fibroin protein powder, which belongs to the field of natural polymers. Background technique [0002] Tussah silkworm cocoons are mainly composed of silk fibroin and sericin, and the degumming process can remove sericin to obtain fibrous tussah silk fibers. Tussah silk fibroin is a typical natural protein polymer with excellent biocompatibility, degradability and low immunogenicity. Because the repeated structural fragment of its primary sequence is similar to that of spider silk traction silk, rich in alanine and β-sheet structure, and has a multi-level structure from microscopic to nanoscopic, it endows tussah silk fibers with supernatural properties. Strong mechanical strength, such as high modulus, tensile strength, etc. At the same time, tussah silk fibroin contains more essential amino...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/14C07K1/34
CPCC07K14/43586
Inventor 帅亚俊古孝雪杨明英
Owner ZHEJIANG UNIV
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