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Preparation method and application of wide-spectrum specific antibody modified magnetic metal organic framework material

A magnetic metal, organic framework technology, applied in the preparation of test samples, analytical materials, alkali metal compounds, etc., can solve the problem of cumbersome antibody preparation process, reduce the impact of detection sensitivity and recovery, simple operation, high The effect of extraction volume

Pending Publication Date: 2021-11-19
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the antibody preparation process is cumbersome, and organophosphates, carbamates, pyrethroids, etc. all have structural commonality, so it is of great practical significance to use broad-spectrum specific antibodies to establish multi-residue immunization pretreatment methods for agricultural and veterinary drugs

Method used

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  • Preparation method and application of wide-spectrum specific antibody modified magnetic metal organic framework material
  • Preparation method and application of wide-spectrum specific antibody modified magnetic metal organic framework material
  • Preparation method and application of wide-spectrum specific antibody modified magnetic metal organic framework material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of broad-spectrum specific antibody-modified MnFe 2 o 4 @MIL-100(Fe) nanoparticles

[0039] Preparation and Characterization of Magnetic MnFe 2 o 4 Magnetic nanoparticles, accurately weigh 0.36mg of FeCl 3 ·6H 2 O and 0.18 mg MnCl 2 4H 2 O was dissolved in 20mL ethylene glycol (EG) / diethylene glycol (DEG) (VEG / VDEG=10 / 10) and magnetically stirred to make it completely dissolved, then weighed 2g polyvinylpyrrolidone (PVP) and added to The mixed solution was fully stirred in an oil bath at 110°C to dissolve it completely. After cooling to room temperature, 2 g of anhydrous sodium acetate was weighed and added, and stirred at room temperature for 40 mins. The above mixed solution was transferred to a polytetrafluoroethylene autoclave, and placed in a blast oven at a controlled temperature of 200°C for 10 h. After the reaction, cool to room temperature, wash the obtained black precipitate with ultrapure water and absolute ethanol three times r...

Embodiment 2

[0044] Example 2: MnFe modified by broad-spectrum specific antibody 2 o 4@MIL-100(Fe) nanoparticle extraction method of trace organophosphorus pesticide residues, the standard solution of pesticide residues is prepared according to the following method: 0.1mg / mL of parathion, methyl parathion, fenitrothion , A solution of fenthion. Accurately weigh 1.00 g of BSA and dissolve it in 20 mL of PBS buffer solution to prepare an adsorption solution of PBS+5% BSA. Accurately weigh 0.375g of glycine into a 50mL volumetric flask, adjust the pH to 3 with dilute hydrochloric acid, and prepare a 0.1mol / L desorption solution.

[0045] 30 mg of the above broad-spectrum specific antibody-modified MnFe 2 o 4 @MIL-100(Fe) nanoparticles are dispersed in the sample solution containing organic phosphorus, including parathion, methyl parathion, fenitrothion and isocarbophos, the concentration of organic phosphorus is 30ng / mL, after Vortex for 60 minutes, and the organic phosphorus is adsorbed...

Embodiment 3

[0046] Example 3: Investigating the influence of immunomagnetic nanoparticles modified with different amounts of broad-spectrum specific antibodies on the enrichment efficiency when extracting organophosphorus pesticide residues, and enriching trace organophosphorus according to the method described in Example 2 Pesticide residues, the difference is that the MnFe modified by the broad-spectrum specific antibody used 2 o 4 The masses of @MIL-100(Fe) immunomagnetic nanoparticles were 10mg, 20mg, 30mg, 40mg, respectively. Along with the increase of above-mentioned immunomagnetic nanoparticles dosage, the extraction efficiency to the added parathion, methyl parathion, fenitrothion and isocarbophos obviously increases, and when the quality of adsorbent reaches 30mg, the extraction efficiency Greater than 96%. Therefore, the quality of the immunomagnetic nanoparticles selected in the present invention is 30 mg.

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Abstract

The invention discloses a preparation method and application of a wide-spectrum specific antibody modified magnetic metal organic framework material. The preparation method comprises the steps: preparing nanoscale magnetic MnFe2O4 nanoparticles, adding the magnetic MnFe2O4 nanoparticles and trimesic acid into N, N-dimethylformamide, evenly stirring, synthesizing a magnetic metal organic framework material MnFe2O4@MIL-100 (Fe) through a hydrothermal method, activating the MnFe2O4@MIL-100 (Fe) by using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), adding a wide-spectrum specific antibody, and carrying out incubation to obtain the wide-spectrum specific antibody modified magnetic metal organic framework material. According to the invention, when the wide-spectrum specific antibody modified magnetic metal organic framework material is used for sample pretreatment, effective enrichment and concentration of various organophosphorus pesticide residues can be realized on the basis of material magnetism and immune performance, and the sample pretreatment process is greatly simplified, so that ultra-sensitive detection of small-molecule hazardous substances in agricultural product samples is realized.

Description

technical field [0001] The present invention relates to the fields of nanomaterial science, immunochemistry and modern separation and analysis, in particular to a method for preparing a magnetic metal organic framework material modified by a broad-spectrum specific antibody, in particular to the preparation of a novel magnetic metal organic framework material and the broad-spectrum antibody Synthesis and modification, suitable for selective separation and enrichment of trace pesticide and veterinary drug residues in complex matrix samples such as environment, food and biology. Background technique [0002] At present, my country's food is rich in variety, sufficient in quantity, and convenient in circulation. However, while meeting the quantity demand, food safety work is facing many difficulties and challenges. All kinds of food safety incidents have reduced the people's trust in food safety, and food safety has become one of the most concerned issues of the masses. The "C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/28B01J20/30B01F7/14B01F1/00C02F1/28G01N1/40G01N1/38C02F101/30B01F27/75
CPCB01J20/06B01J20/226B01J20/28009C02F1/281C02F1/285G01N1/405G01N1/38B01J2220/46B01J2220/4806B01J2220/4812C02F2101/306
Inventor 王建萍俞晓平叶子弘张明洲申屠旭萍付贤树
Owner CHINA JILIANG UNIV
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