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Electrochemical glucose sensor and electrode preparation method thereof

A glucose sensor, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of high cost and large-scale production, inability to accurately and quantitatively detect glucose, and poor detection results.

Pending Publication Date: 2021-11-02
可孚医疗科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, electron mediators made of oxygen, organic dyes, or quinones cannot achieve accurate quantitative detection of glucose, while osmium compounds have disadvantages such as difficult synthesis and high cost, and are not suitable for large-scale production.
At present, there are attempts to use ferrocene and its derivatives in electrochemical glucose sensors, but the detection effect is not good because the content of ferrocene is too low

Method used

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  • Electrochemical glucose sensor and electrode preparation method thereof
  • Electrochemical glucose sensor and electrode preparation method thereof

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specific Embodiment 1

[0020] Take 14 μL of ferrocene polymer solution with a concentration of 5 mg / mL, 6 μL of a solution of glucose oxidase with a concentration of 10 mg / mL, and 0.75 μL of a solution of ethylene glycol diglycidyl ether (EGDGE) with a concentration of 5% v / v. Mixed and allowed to stand for 0.5 hours.

[0021] Take 3 μL of the above mixed solution and drop-coat it on the surface of the glassy carbon electrode, and place the electrode in a laminar flow hood at 4°C for 18 hours to complete the cross-linking reaction.

[0022] After the cross-linking reaction is completed, the electrode is immersed in PBS buffer (pH=7) to remove non-cross-linked components.

[0023] Further, the electrode can be soaked in 0.1MPBS (pH=7.4) buffer solution for storage until use, and the storage temperature is 4°C.

[0024] Wherein, those skilled in the art know that there can be multiple methods for preparing ferrocene polymer solution, for example:

[0025] 1. Dissolve 200mg of BPEI (polyethyleneimine...

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Abstract

The invention discloses a novel electrochemical glucose sensor based on a ferrocene polymer and a preparation method of an electrode thereof. A core element of the glucose sensor is prepared by blending and dispensing a ferrocene polymer electronic mediator, glucose oxidase and polyethylene glycol glycidyl ether according to a certain proportion. Compared with the prior art, sensitivity, reliability and stability of the sensor can be improved, the longer glucose response time can be obtained, and the sensor is suitable for the fields of continuous blood glucose detection, disposable blood glucose test strip detection and the like.

Description

technical field [0001] The invention relates to an electrochemical glucose sensor, in particular to an electrochemical glucose sensor based on a ferrocene polymer and an electrode preparation method thereof. Background technique [0002] Diabetes is a growing concern, and the demand for accurate blood glucose detection devices for clinical diagnosis and personal care is growing exponentially. With the advancement of technology, the electrochemical glucose sensor used for blood glucose detection, as an important electrochemical biosensor, has appeared in people's production and life in large numbers. An electrochemical glucose sensor is an instrument that is sensitive to glucose and converts its concentration into an electrical signal for detection. The substance to be measured enters the bioactive material through diffusion, and undergoes a biochemical reaction with the electronic mediator through molecular recognition. The information generated is then converted into a qua...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327C08G73/02
CPCG01N27/26G01N27/3275C08G73/0206
Inventor 张敏
Owner 可孚医疗科技股份有限公司
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