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Preparation method and application of up-conversion luminescence flexible biosensor for diethylstilbestrol detection

A technology of biosensor and diethylstilbestrol, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of being unable to meet the fast, convenient and economical detection requirements of DES content, threatening the health of consumers, and the operation is too professional, so as to eliminate the background Interference of fluorescence and other molecules, protection of food and environmental safety, high detection accuracy and sensitivity

Active Publication Date: 2021-11-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, China's national standard NY5070-2002 stipulates that DES should not be detected in fishery drug residues in aquatic products, but potentially illegally added DES still threatens the health of consumers
Previous DES detection methods, such as high-performance liquid chromatography, enzyme-linked immunosorbent assay, etc., have difficulties such as professional operation, high detection cost, and cumbersome steps, so they cannot meet the requirements of fast, convenient and economical detection of DES content

Method used

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  • Preparation method and application of up-conversion luminescence flexible biosensor for diethylstilbestrol detection
  • Preparation method and application of up-conversion luminescence flexible biosensor for diethylstilbestrol detection
  • Preparation method and application of up-conversion luminescence flexible biosensor for diethylstilbestrol detection

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Effect test

Embodiment 1

[0044] Step 1, preparation of oleic acid-coated upconversion nanomaterials: Accurately weigh 0.2412g of yttrium chloride hexahydrate, 0.0775g of ytterbium chloride hexahydrate, 0.0019g of thulium chloride hexahydrate and ultrasonically disperse them in 10mL of methanol solvent, add 6mL of oil After acid and 15mL of 1-octadecene, the mixture was reacted with magnetic stirring at 160°C for 30min under nitrogen protection. After cooling to room temperature, a mixed solution containing 0.1g of sodium hydroxide and 0.1482g of ammonium fluoride dissolved in 10mL of methanol was added dropwise. Seal the flask and heat at 80°C for 40min to volatilize methanol, then magnetically stir at 300°C for 1h, after cooling to room temperature, centrifuge to obtain the precipitate of upconversion nanoparticles, and wash three times with a mixed solution of cyclohexane and ethanol with a volume ratio of 1:1 , dried to obtain pure oleic acid-coated upconversion nanoparticles;

[0045] figure 2 i...

Embodiment 2

[0059]Step 1, preparation of oleic acid-coated upconversion nanomaterials: Accurately weigh 0.2412g of yttrium chloride hexahydrate, 0.0775g of ytterbium chloride hexahydrate, 0.0019g of thulium chloride hexahydrate and ultrasonically disperse them in 10mL of methanol solvent, add 6mL of oil After acid and 15mL of 1-octadecene, the mixture was reacted with magnetic stirring at 160°C for 30min under nitrogen protection. After cooling to room temperature, a mixed solution containing 0.1g of sodium hydroxide and 0.1482g of ammonium fluoride dissolved in 10mL of methanol was added dropwise. Seal the flask and heat at 50°C for 40min to volatilize methanol, then magnetically stir at 300°C for 1h, after cooling to room temperature, centrifuge to obtain precipitates of upconversion nanoparticles, and wash three times with a mixed solution of cyclohexane and ethanol with a volume ratio of 1:1 , dried to obtain pure oleic acid-coated upconversion nanoparticles;

[0060] Step 2, preparat...

Embodiment 3

[0067] Step 1, preparation of oleic acid-coated upconversion nanomaterials: Accurately weigh 0.2412g of yttrium chloride hexahydrate, 0.0775g of ytterbium chloride hexahydrate, 0.0019g of thulium chloride hexahydrate and ultrasonically disperse them in 10mL of methanol solvent, add 6mL of oil After acid and 15mL of 1-octadecene, the mixture was reacted with magnetic stirring at 160°C for 30min under nitrogen protection. After cooling to room temperature, a mixed solution containing 0.1g of sodium hydroxide and 0.1482g of ammonium fluoride dissolved in 10mL of methanol was added dropwise. Seal the flask and heat at 70°C for 40min to volatilize the methanol, then magnetically stir at 300°C for 1h, after cooling to room temperature, centrifuge to obtain the precipitate of upconversion nanoparticles, and wash three times with a mixed solution of cyclohexane and ethanol with a volume ratio of 1:1 , dried to obtain pure oleic acid-coated upconversion nanoparticles;

[0068] Step 2, ...

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Abstract

The invention belongs to the field of food and environment safety detection, and particularly relates to a preparation method and application of an up-conversion luminescence flexible biosensor for diethylstilbestrol detection. The preparation method comprises the following steps: step 1, preparing an oleic acid coated up-conversion nano material; step 2, preparing an aminated up-conversion nano material; step 3, preparing a flexible up-conversion luminescence sensor; and step 4, preparing a bio-functionalized flexible up-conversion luminescence sensor. The sensor is used for detecting diethylstilbestrol, and detection of diethylstilbestrol content in food and environmental samples is realized by establishing a diethylstilbestrol content detection standard curve; through preparation of the up-conversion luminescence flexible biosensor and a detection method of diethylstilbestrol, rapid and economical detection of diethylstilbestrol in food and environmental samples is realized, and the up-conversion luminescence flexible biosensor has a wide concentration detection range and a low detection limit; meanwhile, the sensor has a renewable function and has a good practical prospect.

Description

technical field [0001] The invention belongs to the technical field of food and environment detection, and in particular relates to a preparation method and application of an up-conversion luminescence flexible biosensor for diethylstilbestrol detection. Background technique [0002] Diethylstilbestrol (DES) is an endocrine disruptor similar to estrogen. It was originally used to prevent premature birth, miscarriage and pregnancy complications, and later used as a veterinary drug to promote animal growth, but because it interferes with the synthesis and metabolism of normal hormones , thereby posing a potential threat to human health. [0003] DES can cause many adverse effects on human health, such as adverse reproductive disorders, long-term changes in sexual behavior, cervical intraepithelial neoplasia, etc. Therefore, China, the United States and other countries strictly prohibit the detection of DES in food. For example, China's national standard NY5070-2002 stipulates...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 陈全胜吴继忠欧阳琴尹海宁张明明张金贵
Owner JIANGSU UNIV
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