Specific nitrite fluorescence detection method based on copper nanoclusters and oxidation reaction

A technology of nitrite and copper nanoclusters, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of poor portability, complicated pretreatment, high cost, etc., and achieve rapid specific detection, high sensitivity specific detection, strong The effect of fluorescence responsiveness

Pending Publication Date: 2022-04-05
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these detection methods are high in cost, poor in portability, and complicated in pretreatment, which cannot meet the requirements of on-site rapid detection.

Method used

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  • Specific nitrite fluorescence detection method based on copper nanoclusters and oxidation reaction
  • Specific nitrite fluorescence detection method based on copper nanoclusters and oxidation reaction
  • Specific nitrite fluorescence detection method based on copper nanoclusters and oxidation reaction

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Experimental program
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Effect test

Embodiment 1

[0026] Preparation of copper nanoclusters: 125 μl CuCl 2 The solution was mixed with 20ml of 0.21 mM GSH solution under vigorous stirring for 5 min to form a copper thiol complex, at which point the mixed solution gradually became cloudy. Then, 450 μl freshly prepared 100 mM AA solution was added to the mixed solution, heated to 65 °C and continued to stir for 4 h. During this period, the solution gradually became clear, and then the solution was cooled at room temperature to obtain a Cu NCs suspension. The resulting Cu NCs suspension was stored at 4 °C for further use. The preparation and application route of Cu NCs are shown in Fig. 1.

Embodiment 2

[0028] Copper nanoclusters for the detection of nitrite: taking NO 2- Mix it with an acetic acid buffer solution with a pH of 3 and place it in a centrifuge tube, then add Fe at a concentration of 150 μM to the mixture 2+ solution, vortexed to make it fully mixed, after that, 200 μl of the prepared Cu NCs was added to the above solution, and the mixture was incubated at room temperature for 30min, the detection mechanism is as follows: figure 2 shown.

Embodiment 3

[0030] Establishment of standard curve for nitrite detection: Add solutions of different concentrations of nitrite into the specific detection system, measure the fluorescence intensity value at 432nm under the excitation of 365nm excitation light, and record the fluorescence intensity with or without nitrite after incubation Change value, construct the standard curve of the relationship between the fluorescence intensity quenching amount of nano-clusters and the concentration of nitrite in the detection method, its linear range and standard curve are as follows image 3 shown.

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Abstract

The invention discloses a specific nitrite fluorescence detection method based on a copper nano-cluster and an oxidation reaction, and belongs to the technical field of nano-material preparation and nitrite detection. The method comprises the following steps: firstly, preparing a copper nano-cluster by using glutathione as a stabilizer and ascorbic acid as a reducing agent through a one-pot method, and constructing a fluorescence sensor for detecting nitrite on the basis of an oxidation-reduction reaction; under an acidic condition, Fe < 2 + > can be easily oxidized into Fe < 3 + > due to existence of NO2, and quantitative analysis of NO2 <-> can be easily realized by combining a Fe < 2 + >/Fe < 3 + > oxidation-reduction process with fluorescence quenching of Fe < 3 + > on Cu NCs. According to the invention, the sensitivity and accuracy of nitrite detection can be improved, and the sensor has good specificity.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and nitrite detection, and in particular relates to a specific nitrite fluorescence detection method based on copper nano-clusters and oxidation reaction. Background technique [0002] Nitrite is one of the most common nitrogen-containing compounds in nature. It not only exists in the natural environment, but also is widely used in food and chemical industries in human society. However, nitrite has been harmful to humans because of its potential toxicity. nitrite ion has been considered as a class A inorganic pollutant in drinking water: after the interaction between nitrite ion and protein, it is an important precursor to produce highly carcinogenic N-nitrosamine; excessive Nitrite intake can lead to a series of medical problems such as esophageal cancer, infant methemoglobinemia (blue baby syndrome), spontaneous abortion, and birth defects of the central nervous system; the US E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 吕海霞范丹阳宋昊天
Owner FUZHOU UNIV
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