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Microbial composition for preventing or treating type II diabetes mellitus as well as preparation method and application thereof

A microbial composition and diabetes technology, applied in the field of biomedicine, can solve problems such as discomfort, difficulty in controlling blood sugar of patients, and affecting patients, and achieve the effect of great application prospects

Active Publication Date: 2021-11-02
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs cannot fundamentally cure diabetes. At the same time, various therapeutic drugs have their adverse reactions: such as hypoglycemia events and weight gain (such as: insulin and insulin secretagogues, etc.), gastrointestinal reactions ( Such as: biguanides, glycosidase inhibitors and GLP-1 receptor agonists, etc.), weight gain, edema or adverse cardiac events (such as: thiazolidinedione drugs TZDs), etc.
In addition, long-term use of metformin can cause discomfort in the gastrointestinal tract of some patients and may affect the absorption of vitamin B12. Rosiglitazone can cause liver damage and edema, etc.
As a result, the patient developed tolerance to the disease, so that only taking a large dose of hypoglycemic drugs can effectively reduce blood sugar. However, long-term use of insulin will also make the patient dependent on the drug, which is still a temporary solution, not a permanent cure. Difficult to effectively control blood sugar, prone to repeated

Method used

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  • Microbial composition for preventing or treating type II diabetes mellitus as well as preparation method and application thereof
  • Microbial composition for preventing or treating type II diabetes mellitus as well as preparation method and application thereof
  • Microbial composition for preventing or treating type II diabetes mellitus as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0056] (1) Preparation of Bifidobacterium longum BL21 freeze-dried powder:

[0057] Inoculate Bifidobacterium longum BL21 into the medium according to the inoculum amount accounting for 3% of the total mass of the medium, and culture it at 37°C for 18 hours to obtain a culture solution; centrifuge the culture solution to obtain bacteria; protect the bacteria by freeze-drying The resuspension was resuspended (the mass ratio of the lyoprotectant to the bacteria was 2:1) to obtain the resuspension; the resuspension was lyophilized by vacuum freezing method to obtain the lyophilized powder of Bifidobacterium longum (Bifidobacterium longum) BL21 . Reconstituted with water to make 5×10 9 CFU / mL bacterial solution was administered to type II diabetic rats for 12 consecutive weeks.

[0058] (2) Preparation of Lactobacillus rhamnosus Ra05 freeze-dried powder:

[0059] Lactobacillus rhamnosus Ra05 was inoculated into the medium according to the inoculum amount accounting for 3% of th...

Embodiment 1

[0071] Effects of Microbial Composition on Body Weight in Type II Diabetic Rats:

[0072] (1) Grouping situation:

[0073] 54 male SD rats (6 weeks old, body weight between 170g-190g), 3-4 per cage, the feeding temperature was strictly controlled at 22±2°C, the light / dark cycle was 12h, and the rats had free access to food and water. After 2 weeks of adaptive feeding, the rats were randomly divided into CTL group (n=6), fed with common feed; high-fat diet group (n=48), fed with high-fat diet (88% normal feed, 10% Lard, 1.5% cholesterol, 0.5% bile salts), were further divided into type II diabetes group (D2M group, n=6) and intervention 1-7 group (BL21+LRa05 group, n=6).

[0074] (2) Establishment of type II diabetes rat model:

[0075] In the third week, all rats were fasted without food and water for 12 hours. Except for the CTL group, the rats in the other groups were injected with freshly prepared streptozotocin STZ (50 mmol / L) at 100 mg / kg body weight. A certain amount ...

Embodiment 2

[0082] Effects of microbial composition on fasting blood glucose and OGTT in type II diabetic rats:

[0083] The grouping situation, establishment of the type II diabetes rat model and the experimental process were consistent with those in Example 1.

[0084] The fasting blood glucose was measured after the rats were fasted for 12 hours, and the postprandial blood glucose was measured after 2 hours of eating. During the oral glucose tolerance test (OGTT), all rats were fasted overnight, and fasting blood glucose was collected before glucose administration (time = 0 min) as a baseline value. Then, a 2 g / kg glucose solution was administered by oral gavage. Blood samples were collected 15, 30, 60 and 120 min after glucose administration. Blood glucose levels were measured and the area under the glucose curve (AUC) was calculated.

[0085] The results are shown in Table 2 and Table 3. It can be seen from Table 2 that compared with the D2M group, the glucose level in the interve...

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Abstract

The invention relates to a microbial composition for preventing or treating type II diabetes mellitus as well as a preparation method and application thereof. The microbial composition for preventing or treating type II diabetes mellitus comprises a bifidobacterium longum BL21 strain and a lactobacillus rhamnosus LRa05 strain. The microbial composition can obviously reduce the body weight of a patient with type II diabetes mellitus; the levels of ALT, AST and the like in the serum of the type II diabetes mellitus patient are obviously reduced; the levels of serum inflammatory factors such as TNF-alpha and IL-1beta are reduced; the increased fasting blood glucose level and the area under the curve in the oral glucose tolerance test are significantly reduced; the DX-4000-FITC level of the plasma at the time of 2.5 hours is obviously reduced and the plasma LPS level is obviously reduced. Therefore, the microbial composition has a great application prospect in preparation of medicines, foods or health-care products for preventing, treating and relieving type II diabetes mellitus.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a microbial composition and its preparation method and application, in particular to a microbial composition for preventing or treating type II diabetes and its preparation method and application. Background technique [0002] Type II diabetes is the most common type of diabetes, accounting for approximately 90% of all diabetes cases. In type 2 diabetes, hyperglycemia results from insufficient insulin secretion and the body's inability to respond adequately to insulin, defined as insulin resistance. During a state of insulin resistance, insulin does not work well, so an increase in insulin secretion is induced initially to reduce the increase in blood sugar levels, but over time a condition of relative insulin deficiency may continue to develop. [0003] Type 2 diabetes usually has a slow onset and usually does not present with the acute metabolic derangements of type 1 diabete...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L2/385A23L2/52A61K35/747A61K35/745A61P3/10C12R1/01C12R1/225
CPCC12N1/20A23L33/135A23L2/385A23L2/52A61K35/747A61K35/745A61P3/10A23V2002/00A23V2400/175A23V2400/533A23V2200/328A61K2300/00
Inventor 方曙光盖忠辉朱建国王艳丽
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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