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Tea polyphenol-metal nanoparticles, drug-loaded nanoparticles as well as preparation method and application of tea polyphenol-metal nanoparticles and drug-loaded nanoparticles

A technology of metal nanoparticles and drug-loaded nanoparticles, which is applied in the field of medicine to achieve the effects of reducing pollution, good resolubility, and solving cumbersome treatment processes

Pending Publication Date: 2021-11-02
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(Shen Z, Liu T, Li Y, Lau J, Yang Z, Fan W, et al. Fenton-Reaction-Acceleratable Magnetic Nanoparticles for Ferroptosis Therapy of Orthotopic Brain Tumors. ACS nano 2018; 12:11355-65. Liu T, Liu W, Zhang M, Yu W, Gao F, Li C, et al. Ferrous-Supply-Regeneration Nanoengineering for Cancer-Cell-Specific Ferroptosis in Combination with Imaging-Guided Photodynamic Therapy. ACS nano2018; 12:12181-92.), however, Based on EGCG and Fe 3+ Nanoparticles formed between induce ferroptosis pathway and have not been reported for the treatment of lung cancer

Method used

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  • Tea polyphenol-metal nanoparticles, drug-loaded nanoparticles as well as preparation method and application of tea polyphenol-metal nanoparticles and drug-loaded nanoparticles
  • Tea polyphenol-metal nanoparticles, drug-loaded nanoparticles as well as preparation method and application of tea polyphenol-metal nanoparticles and drug-loaded nanoparticles
  • Tea polyphenol-metal nanoparticles, drug-loaded nanoparticles as well as preparation method and application of tea polyphenol-metal nanoparticles and drug-loaded nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] In this embodiment, tea polyphenol-metal nanoparticles are prepared, and the preparation process is as follows:

[0053] (1) Dissolve 20mg EGCG in 10mL deionized water, heat to aid dissolution, and after fully dissolving, prepare an aqueous solution of tea polyphenols with a concentration of 2mg / mL:

[0054] (2) 2.7mg FeCl 3 ·6H 2 Add O into 10mL deionized water, fully dissolve, and prepare 1mM FeCl 3 Aqueous solution:

[0055] (3) FeCl in step (2) 3 Aqueous solution is slowly added dropwise in the tea polyphenol solution in step (1), finally makes FeCl 3 The volume ratio of the aqueous solution to the EGCG storage solution is 1:19, stirring while adding dropwise, and after reacting for 1 hour, collect the product, 8000 rpm, and centrifuge at 4 degrees for 30 minutes, collect the precipitate, and obtain F1 nanoparticles (the obtained tea polyphenols prepared in this embodiment - metal nanoparticles referred to as F1). TEM photo see figure 1 a.

Embodiment 2

[0057] In this embodiment, tea polyphenol-metal nanoparticles are prepared, and the preparation process is as follows:

[0058] (1) Dissolve 20mg of EGCG powder in 10mL of deionized water, heat to help dissolve, and after fully dissolving, prepare an aqueous solution of tea polyphenols with a concentration of 2mg / mL:

[0059] (2) 2.7mg FeCl 3 ·6H 2 Add O into 10mL deionized water, fully dissolve, and prepare 1mM FeCl 3 aqueous solution;

[0060] (3) FeCl in step (2) 3 Aqueous solution is slowly added dropwise in the tea polyphenol aqueous solution in step (1), finally makes FeCl 3The volume ratio of the aqueous solution to the EGCG storage solution is 1:99. Stir while adding dropwise. After reacting for 1 hour, collect the product, centrifuge at 8000 rpm for 30 minutes at 4 degrees, collect the precipitated product, and obtain F3 nanoparticles (tea polyphenols prepared in this example) -Metal nanoparticles are referred to as F3). TEM photo see figure 1 B; see photos of ...

Embodiment 3

[0062] In this example, the DOX drug-loaded tea polyphenol-metal nanoparticles were prepared, and the preparation process was as follows:

[0063] (1) Accurately weigh 4.000 mg of DOX powder, dissolve it in 2 mL of deionized water, and ultrasonically assist the dissolution, so that the final DOX concentration is 2 mg / mL.

[0064] (2) Slowly add the DOX solution in step (1) to the obtained tea polyphenol aqueous solution prepared in step (1) of Example 2, and keep stirring, then add step (2) of Example 2 to prepare the obtained FeCl 3 In aqueous solution, FeCl 3 The volume ratio of the aqueous solution to the EGCG storage solution is 1:99, the theoretical drug loading of DOX is 5%, magnetically stirred at room temperature for 1 hour, the product is collected, centrifuged at 8000 rpm, 4 degrees for 30 minutes, and the precipitate is collected to obtain the drug-loaded nanoparticle DF3 nanometer (the drug-loaded nanoparticles prepared in this example are referred to as DF3). Th...

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Abstract

The invention belongs to the field of medicines, and relates to a tea polyphenol-metal nanoparticle, a drug-loaded nanoparticle as well as a preparation method and application thereof, in particular to application of dual response of tea polyphenol through pH and glutathione to regulation of apoptosis / ferroptosis pathway in the anti-tumor research aspect. The invention provides a drug carrying system for delivering chemotherapeutic drugs, namely tea polyphenol-metal nanoparticles, aiming at cancer treatment. The tea polyphenol-metal nanoparticles are obtained by dropwise adding a metal ion storage solution into an EGCG storage solution, stirring, centrifuging and collecting precipitates; and the metal ion storage liquid and an anti-tumor drug are dropwise added into the EGCG storage liquid, the precipitate is stirred, centrifuged, and collected to obtain the drug-loaded nanoparticles. According to the medicine carrying system, the anti-tumor medicine and metal elements are simultaneously delivered to tumor tissues and are taken by tumor cells, the tea polyphenol-metal nanoparticle structure is depolymerized under the conditions of weak acidity of tumors and high-level glutathione, free metal elements are released, and the anti-tumor medicine achieves the treatment effect.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a tea polyphenol-metal nanoparticle, a drug-loaded nanoparticle and its preparation method and application, specifically to regulate cell apoptosis / ferroptosis by dually responding to tea polyphenols through pH and glutathione Approaches to antitumor applications. Background technique [0002] With the rapid economic growth, the deterioration of the surrounding environment has also been aggravated, resulting in an increasing cancer rate. Tumors generally refer to cells that are stimulated by some external and internal factors and other carcinogenic factors, resulting in gene mutations, unregulated growth, and further formation of local tumors. Tumors are divided into benign and malignant. Benign tumors generally do little harm to the body, while malignant tumors can be life-threatening. In the treatment of malignant tumors, scientists continue to explore. In recent years, many new cance...

Claims

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Application Information

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IPC IPC(8): A61K33/26A61K31/353A61K31/704A61K31/12A61K47/69A61K47/54A61K47/52A61P35/00A61P11/00B82Y5/00B82Y40/00A61L31/16A61L31/02A61L31/00
Inventor 郭刚母敏王跃龙周良学仝爱平
Owner SICHUAN UNIV
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