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Lysate for detecting chlamydia trachomatis antigen and detection method

A Chlamydia trachomatis and antigen detection technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of transportation and detection operation hazards, antigen destruction, affecting sensitivity, etc., to reduce corrosion damage, reduce operation difficulty, and improve sensitivity Effect

Pending Publication Date: 2021-10-29
固安林科特生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the acid-base neutralization scheme, dilute solutions of strong acid and strong base are used, and inaccurate dosage control will lead to antigen destruction and affect sensitivity
At the same time, there are certain potential hazards to relevant personnel in transportation and testing operations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A lysate for chlamydia trachomatis antigen detection, which consists of antigen extraction solution (liquid A) and sample buffer solution (liquid B).

[0032] In the antigen extraction solution, glycerin was selected as solvent, and the addition amount was 80 g; the surfactant was Tween 80 and polyvinylpyrrolidone, and the addition amount was 1 g and 2 g respectively, and the lipid extraction agent was cholesterol, and the addition amount was 5 g. Add surfactants, lipid extractants and solvents in order, and stir and mix at room temperature.

[0033] In the sample buffer solution, the tris buffer in the buffer system includes 1.2 g of tris, the citric acid buffer includes 0.96 g of citric acid, and the pH is 7.5; the surfactant includes lauryl sulfate Sodium: 5g, Triton-100: 5g; the metal ion salt is sodium chloride 1.5g; macromolecular compounds include polyethylene glycol 2.2g, casein sodium 1.5g and bovine serum albumin 0.5g; purified water 100g (100mL ). Add in th...

Embodiment 2

[0035] A lysate for chlamydia trachomatis antigen detection, which consists of antigen extraction solution (liquid A) and sample buffer solution (liquid B).

[0036] In the antigen extraction solution, the solvent includes glycerin, the addition amount is 50g, and the purified water, the addition amount is 30g; the surfactant is Tween 80, 1g, polyvinylpyrrolidone 2g; the lipid extraction agent is high-density lipoprotein 3g. Add surfactants, lipid extractants and solvents in order, and stir and mix at room temperature.

[0037] In the sample buffer solution, the buffer system is a tris buffer, including 1.2 g of tris, boric acid-borax buffer (1 g of boric acid, 0.25 g of borax), and the pH is 7; surfactants include ten 10 g of sodium dialkyl sulfate; 0.5 g of magnesium chloride and 1 g of sodium chloride are selected as metal ion salts; macromolecular compounds include 2.5 g of polyethylene glycol, 1 g of sodium casein and 1 g of bovine serum albumin; 100 g (100 mL) of purifie...

Embodiment 3

[0039] A lysate for chlamydia trachomatis antigen detection, which consists of antigen extraction solution (liquid A) and sample buffer solution (liquid B).

[0040] In the antigen extract solution, the solvent is dimethyl sulfoxide, and the addition amount is 75g; the surfactant is polyvinylpyrrolidone 2g, and the lipid extractant is phenol 5g. Add surfactants, lipid extractants and solvents in order, and stir and mix at room temperature.

[0041]In the sample buffer solution, the buffer system is a tris buffer, including 1.2 g of tris, a citric acid buffer, including 0.96 g of citric acid, and the pH is 7.5; the surfactant includes dodecyl Sodium sulfate 5g, Tween 80, 5g; metal ion salt selected ferrous sulfate 1g; macromolecular compounds including polyvinylpyrrolidone 0.5g, casein sodium 1.5g and bovine serum albumin 0.5g; purified water 100g (100mL). Add in the order of buffer system, macromolecular compound, surfactant, metal ion salt and purified water, and stir and mi...

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PUM

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Abstract

The invention provides a lysate for detecting a chlamydia trachomatis antigen and a detection method, and relates to the technical field of biological detection. The invention provides a lysate for detecting a chlamydia trachomatis antigen. The lysate comprises an antigen extracting solution and a sample buffer solution, the antigen extracting solution comprises a solvent, a surfactant and a lipid extracting agent, and the sample buffer solution comprises a buffer solution, a surfactant, metal ions, macromolecular compounds and a solvent. The lysate is safe, efficient and convenient to transport and store, and the use difficulty of an operator is reduced; in addition, by using the detection method of the lysate provided by the invention, the sensitivity of chlamydia trachomatis antigen detection is improved.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a lysate and a detection method for detecting chlamydia trachomatis antigens. Background technique [0002] Chlamydia trachomatis is a microbe that parasitizes in cells, often parasitizing the eyes and reproductive tract, causing infections of the genitourinary tract. Determining the type of infectious pathogen has important clinical value for the diagnosis and treatment of related diseases. [0003] At present, among clinical patients, 70% to 80% of female patients and 50% of male patients usually show no clinical symptoms, so clinical testing of antigen typing plays an important auxiliary role in diagnosis. Common laboratory inspection methods include: 1) smear microscopy; 2) PCR nucleic acid amplification test; 3) antigen detection; 4) serological testing, etc. Using the nitrocellulose membrane-based chromatography method can provide reliable auxiliary diagnosti...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/569
CPCG01N33/558G01N33/569
Inventor 杨玮史准
Owner 固安林科特生物工程有限公司
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