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Polypeptide, pharmaceutical composition containing polypeptide and application of polypeptide and pharmaceutical composition

A composition and drug technology, applied in the field of polypeptides and pharmaceutical compositions containing the polypeptides and biological drugs, can solve the problems of prolonging half-life, imperfect drug delivery system, poor drug stability, etc.

Pending Publication Date: 2021-10-22
博锐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biological drugs also have some outstanding problems, such as imperfect drug delivery system, poor drug stability, short half-life in vivo and low bioavailability, etc.
Those skilled in the art sometimes optimize the metabolic kinetics of biological drugs through modification to achieve the purpose of prolonging the half-life. However, not all drugs can be modified, and some drugs will lose part or all of their activity during the modification process.

Method used

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  • Polypeptide, pharmaceutical composition containing polypeptide and application of polypeptide and pharmaceutical composition
  • Polypeptide, pharmaceutical composition containing polypeptide and application of polypeptide and pharmaceutical composition
  • Polypeptide, pharmaceutical composition containing polypeptide and application of polypeptide and pharmaceutical composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0214] Embodiment 1: the preparation of polypeptide

[0215] The polypeptides involved in this application (eg, SEQ ID No: 1-6) can be produced by fermentation using Escherichia coli as a host cell. The cDNA sequence encoding the polypeptide was ligated into the pET15b plasmid through enzyme digestion, and then transfected into Escherichia coli. The obtained positive clones were subjected to primary fermentation, and then the primary fermentation liquid was moved to a secondary fermenter (500 L), and IPTG induction was performed when the OD value was 2-5. After protein expression, the mycelia were collected and subjected to cell disruption. After the broken mycelia was collected and resuspended, it was purified with a C 8 liquid phase column and lyophilized for future use.

Embodiment 2

[0216] Embodiment 2: Preparation comprises the pharmaceutical composition of small molecule drug and SEQ ID No:3

[0217] Take amoxicillin, dissolve it in physiological saline to form a 1mmol / L solution and mix well, weigh 0.2mmol of the polypeptide shown in SEQ ID No:3, dissolve it in phosphate buffer (pH=3.0) neutralize and fully mix, and mix amoxicillin with the polypeptide shown in SEQ ID No:3 according to the molar ratio of 5:1. At 4°C, sonicate for 5 minutes, then adjust the pH to 7.0-7.3 to obtain a pharmaceutical composition solution, which is lyophilized for use.

Embodiment 3

[0218] Embodiment 3: Preparation comprises the pharmaceutical composition of siRNA and SEQ ID No:3

[0219] Weigh 3nmol of EGFR siRNA freeze-dried powder and dissolve it in 1mL of phosphate buffer (pH=3.0) and mix well, weigh 9μM of the polypeptide freeze-dried powder shown in SEQ ID No:3 and dissolve it in the above-mentioned containing EGFR siRNA lyophilized powder in phosphate buffer solution and thoroughly mixed, then stirred at 4° C. for 1 hour, and lyophilized to obtain a solid powder of the pharmaceutical composition.

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Abstract

The invention provides a polypeptide which comprises S1 and S2, and the S1 and the S2 are connected in series through a peptide bond in the direction from the N end to the C end. S1 is an amino acid sequence as shown in SEQ ID No: 1 or a functional variant of the amino acid sequence; S2 is an amino acid sequence as shown in SEQ ID No: 7 or a functional variant of the amino acid sequence.

Description

technical field [0001] The present application relates to the technical field of biopharmaceuticals, and more specifically, the present application relates to a polypeptide, a pharmaceutical composition comprising the polypeptide and biopharmaceuticals, and a preparation method and use thereof. Background technique [0002] Biological drugs (especially nucleic acid or protein drugs) are widely used to treat many diseases, such as cancer, autoimmune diseases, and AIDS. However, biological drugs also have some outstanding problems, such as imperfect drug delivery system, poor drug stability, short half-life in vivo, and low bioavailability. Those skilled in the art sometimes optimize the metabolic kinetics of biological drugs through modification to achieve the purpose of prolonging the half-life. However, not all drugs can be modified, and some drugs will lose part or all of their activity during the modification process. Therefore, there is an urgent need in this field to f...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61K45/06A61K47/42A61K9/19A61K9/00A61K9/08A61P31/04A61P3/10A61P3/04A61P7/06A61P1/16
CPCC07K14/00A61K45/06A61K47/42A61K9/19A61K9/0019A61K9/08A61P31/04A61P3/10A61P3/04A61P7/06A61P1/16C07K2319/00
Inventor 龚珉
Owner 博锐生物科技有限公司
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