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Exosome and hyaluronic acid combined wound dressing, and preparation method and application thereof

A technology of hyaluronic acid and wound dressing, applied in the direction of bone/connective tissue cells, bandages, vertebrate cells, etc., can solve problems such as disadvantage and hyaluronic acid toxicity, and achieve a strong implementation, not easy to be infected, and promote healing. Effect

Active Publication Date: 2021-10-19
GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hyaluronic acid after cross-linking treatment will be slightly toxic, which is not conducive to the subsequent application as a carrier in the medical field

Method used

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  • Exosome and hyaluronic acid combined wound dressing, and preparation method and application thereof
  • Exosome and hyaluronic acid combined wound dressing, and preparation method and application thereof
  • Exosome and hyaluronic acid combined wound dressing, and preparation method and application thereof

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preparation example Construction

[0068] Correspondingly, the present invention also provides a preparation method of the above-mentioned exosome combined with hyaluronic acid wound dressing, comprising the following steps:

[0069] S1: Isolate and purify mesenchymal stem cell exosomes from the supernatant of the umbilical cord mesenchymal stem cell culture medium, resuspend the mesenchymal stem cell exosomes in PBS, sterilize, and freeze them;

[0070] S2: Repeated freezing and thawing of hyaluronic acid aqueous solution to cause physical crosslinking, and then adding adipic acid dihydrazide and carbodiimide hydrochloride for chemical crosslinking, and adjusting the pH to obtain hyaluronic acid hydrogel glue;

[0071] S3: Wrapping the mesenchymal stem cell exosomes with the hyaluronic acid hydrogel to obtain a wound dressing.

[0072] The preparation method of the present invention includes three major steps of preparing exosomes of mesenchymal stem cells, preparing hyaluronic acid hydrogel, and encapsulatin...

Embodiment 1

[0105] Preparation of wound dressings with exosomes combined with hyaluronic acid, the specific steps are as follows:

[0106] S1: Preparation of exosomes from mesenchymal stem cells:

[0107] 1) The hospital collects the umbilical cord tissue of the full-term cesarean section fetus, signs the customer's informed consent before the collection, and transports the tissue to the laboratory in a refrigerated and sterile environment at 4°C. During the transportation, the tissue protection solution is used to protect the biological activity of the umbilical cord tissue, and the tissue protection solution Prepared by adding 25 μg / ml gentamicin sulfate and 5 μg / ml amphotericin B to normal saline to ensure no bacterial and fungal contamination during transportation;

[0108] 2) In the laboratory, use tissue protection solution to clean the blood on the surface of the umbilical cord tissue, remove the epidermis and vascular tissue, take out the Huatong glue, wash it, and cut it into pie...

Embodiment 2

[0124] S1: Preparation of exosomes from mesenchymal stem cells:

[0125] Wherein 1)~7) and 9) steps in the S1 step are the same as embodiment 1, no more details, its difference is in step 8):

[0126] Concentrate the liquid by ultracentrifugation, centrifuge at 15,000 rpm for 45 minutes to remove fine cell debris, take the supernatant and centrifuge again at 100,000 rpm for 45 minutes, remove the supernatant, precipitate exosomes secreted by stem cells, add 10ml of normal saline to wash once , centrifuged at 100,000 rpm for 45 minutes, removed the supernatant, and obtained exosome pellets.

[0127] S2: Preparation of hyaluronic acid hydrogel:

[0128] 1) Weigh 1g of hyaluronic acid powder, then add 100ml of deionized water to dissolve, and finally form a hyaluronic acid aqueous solution with a concentration of 1%, stir evenly on a magnetic stirrer, and freeze repeatedly to make it cross-linked. The freezing condition is: store at -25°C for 14 hours; the thawing condition is:...

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Abstract

The invention discloses an exosome and hyaluronic acid combined wound dressing, and a preparation method and application thereof. The wound dressing is formed by wrapping mesenchymal stem cell exosome with hyaluronic acid hydrogel, and the hyaluronic acid hydrogel is obtained by repeatedly freezing and unfreezing a hyaluronic acid aqueous solution and then cross-linking the hyaluronic acid aqueous solution with carbodiimide hydrochloride and adipic dihydrazide. Correspondingly, the invention also discloses a preparation method of the exosome and hyaluronic acid combined wound dressing and application of the exosome and hyaluronic acid combined wound dressing in medicines or materials for promoting wound healing. The wound dressing combines the characteristics of the exosome of the mesenchymal stem cells and the hyaluronic acid hydrogel, can promote revascularization and collagen synthesis after being in contact with a wound surface so as to repair damaged tissues, is safe, reliable and good in biocompatibility, has an obvious promoting effect on healing of the wound surface, and is not easy to cause infection, and no scar is left after healing.

Description

technical field [0001] The invention relates to the technical field of preparation of biomedical materials, in particular to a wound dressing combined with exosomes and hyaluronic acid, a preparation method and application thereof. Background technique [0002] Due to certain systemic diseases, the immune cells of the human body are damaged; old age, infirmity, malnutrition, etc. lead to insufficient cell regeneration ability; factors such as wound infection and inflammatory response often make it difficult for wounds to heal. With the advancement of science and technology, medical clinics are increasingly pursuing efficiency and efficacy in wound care. Traditional dressings such as bandages, gauze, and cotton balls in the past cannot meet the needs of modern clinical treatment. People need more efficient wound recovery medical materials. And dressings that are more suitable for wounds with diverse diseases to protect wounds, promote skin regeneration, and accelerate wound h...

Claims

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Application Information

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IPC IPC(8): A61L26/00C12N5/0775
CPCA61L26/0023A61L26/008A61L26/0057A61L26/0066C12N5/0668A61L2300/30A61L2300/412C08L5/08
Inventor 蒙燕瑶唐淑艳刘小翠杨景利王进辉
Owner GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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