Bioelectrochemical method for real-time in-situ detection of nitrite in sewage

A technology of bioelectrochemistry and nitrite, which is applied in the field of bioelectrochemistry for real-time in-situ detection of nitrite in sewage, can solve the problems of nitrite biosensor performance changes and long incubation time, and achieve shortened preparation time, The effect of good linear relationship and shortened test time

Active Publication Date: 2021-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports of nitrite biosensors using nitrite-reducing bacteria as the detection unit, which may be due to the long-term culture of mature nitrite-reducing biocathodes (usually more than 92 days) and the need for continuous Input electrical energy to sustain growth of electroactive nitrite
In addition, the organic matter contained in the actual sewage is likely to cause irreversible changes in the autotrophic bioelectrochemical reactor, which makes the performance of the nitrite biosensor using nitrite-reducing bacteria as the detection unit may change during operation.

Method used

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  • Bioelectrochemical method for real-time in-situ detection of nitrite in sewage
  • Bioelectrochemical method for real-time in-situ detection of nitrite in sewage
  • Bioelectrochemical method for real-time in-situ detection of nitrite in sewage

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Use the sludge water from the anoxic section of the sewage plant as the inoculum, and cultivate the bioelectrode for the detector in the MFC mode:

[0069] The bioelectrochemical reactor is externally connected with a resistor, and the loop is connected with a copper wire. The bioelectrochemical reactor was inoculated with the sludge water in the anoxic section. The inoculation process lasted for five days, and then it was transferred to the sequence batch mode for cultivation, and the matrix solution was replaced every day. The voltage of the MFC was recorded by a digital data collection instrument (KEYSIGHT model 34970A).

[0070] Specifically: a bioelectrochemical reactor is constructed using a reactor with a cubic two-chamber structure, with the bioelectrode 12 as the anode and the non-biology electrode 13 as the cathode, wherein the anode chamber 14 of the bioelectrochemical reactor has an inner diameter of 35 mm and a length of 40 mm. The volume is 40mL, the inne...

Embodiment 2

[0077] The response current curve of the bioelectrode in the solution containing different nitrite concentrations generated by LSV scanning varies with the potential. According to the curve data, the most suitable signal output potential of the nitrite detection process is selected:

[0078] The bioelectrochemical reactor is connected with the potentiostat 19 with a three-electrode system, graphite electrode (bioelectrode) 12, nickel foam air electrode 13 and silver / silver chloride electrode 11 are used as working electrode, counter electrode and reference electrode respectively ,Such as Figure 3-2 shown. By adding 12.5mL L -1 Trace minerals and 5mL L -1 Add nitrite to vitamin 50mMPBS to prepare a standard nitrite-containing solution, and set the concentration of the standard nitrite solution to 0.1mgNO 2 - -N L -1 ,0.2mgNO 2 - -N L -1 ,0.4mgNO 2 - -N L -1 ,1mgNO 2 - -N L -1 ,2mgNO 2 - -N L -1 ,4mgNO 2 - -N L -1 ,8mgNO 2 - -N L -1 and 16mgNO 2 - -N L ...

Embodiment 3

[0081] Take the present invention as the detection marking line measurement of the bioelectrochemical sensor of detection method in nitrite solution:

[0082] The bioelectrochemical reactor was connected to a potentiostat with a three-electrode system, and graphite electrodes (bioelectrodes), nickel foam air electrodes and silver / silver chloride electrodes were used as working electrodes, counter electrodes and reference electrodes, respectively. By adding 12.5mL L -1 Trace minerals and 5mL -1 Add nitrite to vitamin 50mMPBS to prepare a standard nitrite-containing solution, and set the concentration of the standard nitrite solution to 0.05mgNO 2 - -N L -1 , 0.1mgNO 2 - -N L -1 ,0.2mgNO 2 - -N L -1 ,0.4mgNO 2 - -N L -1 ,1mgNO 2 - -N L -1 ,2mgNO 2 - -N L -1 ,4mgNO 2 - -N L -1 ,8mgNO 2 - -N L -1 and 16mgNO 2 - -NL -1 ,20mgNO 2 - -N L -1 . Select the current at the -0.5V potential of each LSV scanning curve for linear fitting, determine the maximum l...

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Abstract

The invention discloses a bioelectrochemical method for real-time in-situ detection of nitrite in sewage, which comprises the steps of 1) taking a biological electrode as an anode and a non-biological electrode as a cathode, performing closed-circuit operation in an MFC mode, adding an inoculation solution into an anode chamber, continuing the inoculation process for five days, and replacing the inoculation solution every day; 2) culturing the biological electrode in a matrix solution, and replacing the matrix solution every day until an electrode biological membrane grows maturely; (3) connecting a bioelectrochemical reactor with a constant potential rectifier through a three-electrode system, putting the electrode biological membrane into solutions with different nitrite concentrations, carrying out linear voltammetry scanning analysis to obtain an electric response, and selecting an optimal electric response output potential; 4), obtaining a linear equation of the electrical response signal of the bioelectrochemical sensor and the nitrite concentration in solutions with different nitrite concentrations; and 5) obtaining the concentration of nitrite in the detected sewage according to the electric signal of the biological electrode in the detected sewage.

Description

technical field [0001] The invention belongs to the field of biosensor and water body pollutant detection. The invention relates to a pollutant detection technology based on a bioelectrochemical reaction. The bioelectrochemical sensor adopts the detection method to realize real-time in-situ nitrite concentration measurement; in particular, it relates to a bioelectrochemical method for real-time in-situ detection of nitrite in sewage. chemical method. Background technique [0002] Nitrite is a typical toxic pollutant that widely appears in domestic wastewater and industrial wastewater. Nitrite in sewage may increase from low concentration to high concentration with the collection of discharged wastewater and the occurrence of nitrification reaction. usually, The accumulation of nitrite in a small amount of food can cause food poisoning, and the accumulation of nitrite above 3g can cause death. Therefore, in order to avoid possible environmental and human health risks, re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3275G01N27/3277G01N27/327G01N27/48Y02E60/50
Inventor 成少安林朱凡
Owner ZHEJIANG UNIV
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