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High-molecular extract in umbilical cord mesenchymal stem cells, application and detection method

A technology of stem cells and macromolecules, applied in the field of cosmetics, can solve the problems of high production cost and low concentration, and achieve the effects of enhancing repair ability, increasing yield and content of active substances

Pending Publication Date: 2021-10-15
广州市宙斯生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mesenchymal stem cells themselves and their secreted paracrine factors or exosome active substances used in the prior art have low concentrations and high production costs.

Method used

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  • High-molecular extract in umbilical cord mesenchymal stem cells, application and detection method
  • High-molecular extract in umbilical cord mesenchymal stem cells, application and detection method
  • High-molecular extract in umbilical cord mesenchymal stem cells, application and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: A polymer extract from umbilical cord mesenchymal stem cells, which is extracted by the following steps:

[0041] (1) Umbilical cord mesenchymal stem cells were isolated from umbilical cord tissue blocks, and umbilical cord specimens that met the collection standards were collected, and cell separation was performed within 48 hours. Discard the umbilical cord preservation solution, rinse with PBS 3 times, and move the umbilical cord to a Ф10cm petri dish, and cut the umbilical cord into small sections with good transparency, no damage, and no edema area, and cut each section into 4*4cm. Cut the umbilical cord longitudinally with tissue scissors, remove the umbilical vein and umbilical artery, and rinse with PBS four times. Use special scissors for umbilical cord tissue to cut the remaining tissue of the umbilical cord, that is, Wharton's jelly, into 1mm 3 The tissue pieces were inoculated into a new Ф10cm culture dish, half of the serum-free complete medi...

Embodiment 2

[0045] Embodiment 2: The difference from Embodiment 1 lies in step (3):

[0046] (3) Cultivate skin stem cells, and use 50mJ / cm in the logarithmic phase 2 Ultraviolet irradiated cells for 7h;

Embodiment 3

[0047] Embodiment 3: The difference from Embodiment 1 lies in step (3):

[0048] (3) Culture skin stem cells, and use 60mJ / cm in the logarithmic phase 2 Ultraviolet irradiated cells for 7h;

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Abstract

The invention provides a high-molecular extract in umbilical cord mesenchymal stem cells, application and a detection method, and belongs to the technical field of cosmetics. The high-molecular extract is prepared by the following steps: (1) separating umbilical cord mesenchymal stem cells from umbilical cord tissue blocks or umbilical cord blood; (2) directionally differentiating the umbilical cord mesenchymal stem cells obtained in the step (1) into skin stem cells; (3) collecting and splitting the skin stem cells obtained in the step (2), and enriching active medium and high-molecular substances through a molecular sieve with the pore diameter of 4-20.0 nm to obtain the high-molecular extract of the umbilical cord mesenchymal stem cells. The concentration of the high-molecular substance active substances is high, and the anti-allergy and repairing capability on the skin is high.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a polymer extract from umbilical cord mesenchymal stem cells, an application and a detection method. Background technique [0002] With the improvement of living standards and the advancement of science and technology, the basic functions such as whitening and moisturizing in the past can no longer meet people's needs for cosmetics, and anti-aging has gradually become an aspect of everyone's concern. Skin aging is a continuous and gradual physiological process. In appearance, it is manifested as dry skin, sagging, wrinkles, and pigment deposition. The occurrence of aging includes many factors; skin aging is mainly divided into natural aging and photoaging. Natural aging is caused by the internal factors of the body. Oxidized and glycosylated proteins gradually accumulate, resulting in the loss of collagen and elastin in the dermis, and thinning of the skin. It is common in exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/98A61K8/9789A61K8/92A61K8/73A61K8/66A61K8/64A61K8/362A61Q19/08A61Q19/00C12N5/071C12N5/0775C12N13/00G01N30/02G01N30/06G01N30/72
CPCA61K8/99A61K8/988A61K8/9789A61K8/922A61K8/73A61K8/66A61K8/64A61K8/362A61Q19/08A61Q19/004A61Q19/005C12N5/0665C12N5/0625C12N13/00G01N30/02G01N30/06G01N30/72C12N2500/90C12N2500/38C12N2501/125C12N2501/15C12N2501/11C12N2501/115C12N2501/999A61K2800/5922A61K2800/805A61K2800/82A61K2800/85G01N2030/027
Inventor 王锐周豫生许依林
Owner 广州市宙斯生物科技有限责任公司
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