A kind of saline-alkali-resistant bacterial strain kj-1 producing indole acetic acid and its application

A technology of indole acetic acid and KJ-1, applied in the field of agricultural microorganisms, can solve the problems of crop nutrition disorder, root hair cell dehydration, withering, etc., achieve high salt-alkali tolerance, promote seed germination and crop growth, and adapt to temperature range wide effect

Active Publication Date: 2022-06-24
SHIHEZI UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specifically, when the salt content is high, the osmotic pressure of the soil solution is higher than the osmotic pressure of the plant cell solution, which will cause dehydration of the root hair cells, resulting in withering or "burning seedlings"; and high concentration of salt will interfere with the absorption of nutrients by crops, resulting in nutritional disorders of crops

Method used

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  • A kind of saline-alkali-resistant bacterial strain kj-1 producing indole acetic acid and its application
  • A kind of saline-alkali-resistant bacterial strain kj-1 producing indole acetic acid and its application
  • A kind of saline-alkali-resistant bacterial strain kj-1 producing indole acetic acid and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and identification of saline-alkali-tolerant indoleacetic acid-producing strain KJ-1 and strain KC-1

[0029] Soil was collected from saline-alkali land in Xinjiang, packed into clean sampling bags, marked, and stored in a 4°C refrigerator for later use.

[0030] Weigh 10 g of the collected soil sample and place it in a 250 mL conical flask containing 90 mL of sterilized water. After shaking at 150 r / min for 20 min on a shaker, use a 1 mL pipette to draw 1 mL from the supernatant and add it to a 9 mL of sterile water. Mix well in the test tube of bacterial water, then use a pipette to draw 1 mL from this test tube and add it to another test tube containing 9 mL of sterile water, mix well, and so on to make 10. -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 After different dilutions of the solutions, draw 0.1 mL of each and spread them evenly on the LB medium plate containing 10% sodium chloride, invert them in a 30°C incubator for 2-3 days, and pick...

Embodiment 2

[0035] Example 2 Study on growth characteristics of strains KJ-1 and KC-1

[0036] 1. Effects of different pH on the growth of strains KJ-1 and KC-1

[0037] The bacterial suspensions of strains KJ-1 and KC-1 were inoculated into LB medium with different pH values ​​(6, 7, 8, 9, 10, 11) with 1.0% inoculum respectively, and were shaken at 30 ° C and cultured at 150 r / min for 24 h. , with the LB medium without inoculation as the control, the optical density value (D600nm) was detected by 722 spectrophotometer. The results are shown in Tables 1 and 2. The alkali resistance of KJ-1 is better than that of KC-1.

[0038] Table 1 Effects of different pH on the growth of strain KJ-1

[0039]

[0040] Table 2 Effects of different pH on the growth of strain KC-1

[0041]

[0042]

[0043] 2. Effects of different temperatures on the growth of strains KJ-1 and KC-1

[0044] The bacterial suspensions of strains KJ-1 and KC-1 were inoculated in LB medium with a pH value of 9 wit...

Embodiment 3

[0056] Example 3 Qualitative determination of nitrogen fixation of strains KJ-1 and KC-1

[0057] The strains were streaked in Ashbei medium, cultured at 30°C for 7 days, and the qualitative determination of nitrogen fixation was carried out.

[0058] Ashwagandha medium: mannitol 10.0g, KH 2 PO 4 0.2g, MgSO 4·7H 2 O 0.2g, NaCl 0.2g, CaCO 3 5.0g, CaSO 4 ·2H 2 O 0.1 g, agar 20 g, deionized water 1 000 mL, sterilized at 121 °C for 20 minutes.

[0059] It was determined that the strain KJ-1 could grow in this medium, indicating that the strain had nitrogen fixation ability.

[0060] It was determined that the strain KC-1 could not grow in this medium, and the strain had no nitrogen-fixing ability.

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Abstract

The invention discloses a saline-alkali-resistant bacterial strain KJ-1 producing indole acetic acid and its application, which belongs to the field of agricultural microorganisms. The bacterial strain KJ-1 is preserved in the China Center for Typical Culture Collection, and the preservation number is CCTCC NO: M2021332. The strain has high salt-alkali tolerance, can adapt to a wide temperature range, can synthesize more indole acetic acid, has nitrogen fixation, and can promote seed germination and crop growth.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a saline-alkali-tolerant indoleacetic acid-producing strain KJ-1 and its application. Background technique [0002] Soil salinization will affect crop growth, and crops in severe saline-alkali soil areas can hardly survive. Specifically, when the salt content is high, the osmotic pressure of the soil solution is higher than the osmotic pressure of the plant cell fluid, which will cause dehydration of root hair cells, resulting in withering or "burning seedlings"; and high concentrations of salt will interfere with the absorption of nutrients by crops, causing crop nutrition disorders . In addition to its own toxicity to crops, alkaline salts can also produce alkaline reactions, so that phosphate and many nutrients such as iron, zinc, and manganese are fixed in the soil, thereby affecting the absorption and utilization of nutrients by crops, resulting in Calc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G7/06A01C1/00C12R1/06
CPCC12N1/20A01G7/06A01C1/00
Inventor 张凤华赵亚光胡志杰孙福新张成
Owner SHIHEZI UNIVERSITY
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