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Biological reduction strain for kitchen waste and application thereof

A technology for strains and cultures, applied in the field of food waste biological reduction strains, can solve the problems of fewer types of efficient and functional microorganisms, poor reduction effect, slow start-up, etc., to achieve reduction and stabilization, and solve biological reduction. low volume effect

Active Publication Date: 2022-05-24
上海康鸿凯科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the biological reduction of kitchen waste still has problems such as slow start and poor reduction effect. The main reasons for this problem are due to the high temperature of the indigenous microorganisms in the raw materials, few types of microorganisms with high-efficiency functions, low concentration, and poor activity.

Method used

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  • Biological reduction strain for kitchen waste and application thereof
  • Biological reduction strain for kitchen waste and application thereof
  • Biological reduction strain for kitchen waste and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Isolation, screening and performance determination of kitchen waste bioreduction bacteria

[0051] The separation and purification process of Geobacillus xylosus CCTCC NO: M 20211626 strain of the present invention: the test samples were collected from a high-temperature aerobic fermentation tank of a catering waste treatment plant, mixed with sterile water, and then diluted in gradients. Take 200 μL of the diluted solution and spread it on the separation and purification medium plate, and culture it in a constant temperature incubator at 50°C for 16 to 24 hours. Pick single colonies with different shapes and sizes, streak and purify, and store them in numbers. A total of 41 colonies were obtained after preliminary screening. strains;

[0052] The 41 bacterial strains obtained by the preliminary screening were inoculated onto the protease screening medium, amylase screening medium, cellulase screening medium and lipase screening medium respectively by the meth...

Embodiment 2

[0059] Example 2 Preparation of microbial agent for the bioreduction of kitchen waste from Geobacillus xylosus CCTCC NO: M 20211626 strain

[0060] Preparation of liquid bacterial agent: Inoculate the pure strain of CCTCC NO: M 20211626 into 10mL seed medium, cultivate it in a constant temperature shaker at 50°C and 180r / min for 16-24h, and then inoculate it at a weight ratio of 1%. Insert it into the same liquid culture medium to expand the culture, and cultivate it at 50°C and 180r / min for 16-24 hours to obtain the liquid bacterial agent of the strain;

[0061] Preparation of solid bacterial agent: the liquid bacterial agent of the strain is mixed with wood chips, rice husk, wheat bran and other carriers, and dried, wherein the mass ratio of the carrier to the liquid microbial agent is 1:0.1-1:5.

Embodiment 3

[0062] Example 3 Bioreduction treatment effect of Geobacillus xylosus CCTCC NO: M 20211626 strain bacterial agent on different sources of single food waste

[0063] Add the solid bacterial agent of Geobacillus xylosus CCTCC NO: M 20211626 strain to a single food waste raw material from different sources. The mass ratio of the solid bacterial agent to the food waste is 1:2. For high-temperature aerobic fermentation, ventilation, stirring and proper water replenishment are required during the process to ensure that the moisture content of the material is 40-60%, so as to ensure uniform heating and carry out under aerobic conditions. The samples of the processing system are weighed and calculated every 12 hours to obtain The degradation rate of organic matter in kitchen waste, the reduction effect is as follows: figure 2 , image 3 , Figure 4 shown;

[0064] Catering waste is taken from a canteen of a unit, and its organic matter content is about 86.3% by dry weight. figur...

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Abstract

The invention relates to a kitchen waste biological reduction bacterial strain and application thereof, an efficient kitchen waste biological reduction bacterial strain is obtained through primary screening of an isolation culture medium and secondary screening of an enzyme production culture medium, the efficient kitchen waste biological reduction bacterial strain is identified as Geobacter xylosilylus, the efficient kitchen waste biological reduction bacterial strain is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20211626, and the efficient kitchen waste biological reduction bacterial strain is preserved in the China Center for Type Culture Collection (CCTCC). The geobacillus xylosus CCTCC NO: M 20211626 is resistant to high temperature and high salt, has the capability of rapidly and efficiently degrading organic matters in the kitchen waste, can be applied to the biological reduction treatment process of the kitchen waste, and realizes rapid biological reduction and stabilization of the kitchen waste.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a kitchen waste bioreduction bacterial strain and application thereof. Background technique [0002] Kitchen waste is the residual waste after people's meal and the discarded components in the cooking process (such as vegetable leaves and peeled vegetables, etc.), mainly from restaurants, canteens of enterprises and institutions, and other catering industries or households. With the improvement of people's living standards and the continuous advancement of urbanization in our country, the amount of food waste generated is increasing year by year. Especially after the implementation of waste classification in my country in 2019, the amount of food waste generated reached 121 million tons. The main components of food waste are rice, pasta, meat and bone, vegetables and oil. From the chemical composition, it is mainly protein, fat, carbohydrates, v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/01
CPCC12N1/20B09B3/00Y02E50/30
Inventor 茹凯刘雄伟
Owner 上海康鸿凯科技有限公司
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