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Method for rapidly detecting total amount of paraoxon by ultraviolet spectrophotometry

A technology of spectrophotometry and detection method, which is applied in the field of analytical chemical detection, can solve the problems of difficult specific screening and detection, and achieve the effects of environmental friendliness, convenient use and simple preparation

Pending Publication Date: 2021-10-01
BEIJING RES CENT FOR AGRI STANDARDS & TESTING +1
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  • Claims
  • Application Information

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Problems solved by technology

However, because the UV-Vis absorption peak of paraoxon overlaps with the absorption peaks of other pesticides, it is difficult to use the UV-Vis method to achieve specific screening and detection

Method used

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  • Method for rapidly detecting total amount of paraoxon by ultraviolet spectrophotometry
  • Method for rapidly detecting total amount of paraoxon by ultraviolet spectrophotometry
  • Method for rapidly detecting total amount of paraoxon by ultraviolet spectrophotometry

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Effect test

Embodiment 1

[0038] Preparation of the rapid hydrolysis reagent: Weigh 40 mg of sodium hydroxide, 5 mg of sodium sulfate, and 7 mg of potassium chloride, dissolve them in ultrapure water, and set the volume to 10 ml to obtain the rapid hydrolysis reagent, mix well and set aside.

Embodiment 2

[0040] The hydrolysis research of methyl paraoxon and ethyl paraoxon was carried out using the rapid hydrolysis reagent prepared in Example 1. Prepare 1000nM methyl paraoxon and 1000nM ethyl paraoxon solutions with rapid hydrolysis reagent, after vortex mixing, take 100 μL at different reaction time points, and carry out UV spectrophotometric detection respectively, the results are shown in Table 1 .

[0041] Changes of paraoxon concentration with time in table 1 hydrolysis system

[0042]

[0043] The results show that the rapid hydrolysis reagent has a strong hydrolysis effect on methyl paraoxon and ethyl paraoxon, and the concentration of paraoxon in the system decreases significantly with the prolongation of the reaction time. After 30 minutes of reaction, 90% of the methyl paraoxon Both phosphorus and ethyl paraoxon were hydrolyzed.

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Abstract

The invention relates to the technical field of analytical chemistry detection, in particular to a method for rapidly detecting the total amount of paraoxon by ultraviolet spectrophotometry. The method comprises the following steps: S1, preparing a rapid hydrolysis reagent by using ultrapure water, and uniformly mixing; S2, adding the mixed solution in the step S1 into a centrifugal tube, adding paraoxon methyl and paraoxon ethyl into the centrifugal tube at the same time, and uniformly mixing; S3, taking 100 [mu] L of the mixed solution in the step S2, adding a proper amount of acetonitrile, uniformly mixing, and recording as a to-be-detected solution; and S4, preparing nitrophenol solutions with different concentrations, carrying out ultraviolet spectrophotometric detection, and drawing a standard curve according to a determination result. According to the method of the invention, the rapid hydrolysis reagent has the advantages of cheap raw materials, no pollution and environmental friendliness, is simple to prepare and convenient to use, and can effectively improve the hydrolysis efficiency and speed.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry detection, in particular to a rapid detection method of paraoxon total amount by ultraviolet spectrophotometry. Background technique [0002] As we all know, paraoxon pesticides are highly toxic organophosphorus pesticides with broad-spectrum insecticidal effects, and paraoxon residues in the environment have serious harm to the environmental microecology. At present, the detection of paraoxon pesticides mainly uses large-scale instruments, such as gas chromatography, gas chromatography-mass spectrometry, liquid chromatography, liquid chromatography-mass spectrometry, etc. These traditional analysis methods are pesticide residue detection and It is the backbone of quantitative analysis, but there are still some shortcomings such as expensive equipment, high detection cost, long sample pretreatment time and cumbersome process, requiring specialized technical personnel to operate, and i...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 梁刚甄建辉巩文雯李冰茹陈瑞春潘立刚付海龙
Owner BEIJING RES CENT FOR AGRI STANDARDS & TESTING
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