Method for screening athletes by adopting rs283 polymorphic sites of LPL genes, primer group and kit

Abstract

The invention relates to a method for screening athletes by adopting rs283 polymorphic sites of LPL genes, a primer group and a kit. The method comprises the following steps of extracting a DNA template; and and detecting the gene sequences of the rs283 polymorphic sites of the LPL genes in the DNA template to obtain genotypes of the sites on the gene sequences and determine screening results of subjects. According to the screening method, TT genes on the rs283 polymorphic sites are taken as gene markers, T alleles are taken as auxiliary conditions, and when the TT genes exist on the rs283 polymorphic sites of the LPL genes of DNA of the subjects, it can be determined that the subjects have the athletic ability superior to that of ordinary people, so that the subjects can be screened as the athletes; and the method is simple and easy to implement, high in accuracy and efficient, and can also be used for guiding public fitness and improving aerobic exercise ability.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for screening athletes by using the rs283 polymorphic site of LPL gene, a primer set and a kit. Background technique [0002] The polymorphism of the LPL gene has an important impact on human lipid metabolism. The lipoprotein lipase (LPL) encoded by it is synthesized and secreted by parenchymal cells such as adipocytes, cardiomyocytes, skeletal muscle cells, breast cells, and macrophages. A glycoprotein that participates in or affects various metabolic processes related to exercise in the human body. [0003] Studies have shown that the exercise metabolism process of athletes is significantly different from that of the general population, and lipoprotein lipase has an important impact on exercise metabolism, and the individual's exercise ability is largely controlled by the LPL gene. Because LPL has polymorphisms, by analyzing and determining the polymorphic sites of ...

AI Technical Summary

Problems solved by technology

However, for different genes and specific polymorphic sites, different typing and sequencing methods have different accuracy

Gene sequencing is the most accurate, but its high cost and long time make it difficult to be used as a general rapid detection method. Time-of-flight mass spectrometry has the advantages of high throughput, low cost, and high efficiency. However, when it is applied to different genes When polymorphic sites are involved, the accuracy is difficult to guarantee

[0005] Although the above-mentioned gene polymorphic site typing and sequencing technologies have been applied in the process of predicting people's sports potential and screening athletes, there are many genes related to sports ability in the human body, and there are many gene markers that may be involved At the same time, the athletic ability of the human body and whether it has the potential to be an athlete are often determined by various factors. In the prior art, direct detection of the genes related to the athletic ability itself often leads to the problem of low accuracy of the results

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