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Echinacea purpurea extract, and preparation method and applications thereof in metabonomics

A technology of echinacea and extract, applied in the field of natural medicinal chemistry, can solve problems such as hidden safety hazards and poor disease treatment effect, and achieve the effects of improving growth performance and enhancing immune cell activity

Pending Publication Date: 2021-09-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The first object of the present invention is to provide a kind of Echinacea purpurea extract in view of the above-mentioned deficiencies in the prior art, and the technical problem to be solved is to overcome the poor therapeutic effect and potential safety hazards of existing chemical medicines on diseases

Method used

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  • Echinacea purpurea extract, and preparation method and applications thereof in metabonomics
  • Echinacea purpurea extract, and preparation method and applications thereof in metabonomics
  • Echinacea purpurea extract, and preparation method and applications thereof in metabonomics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for preparing an extract of Echinacea purpurea, which comprises the following steps: take 1000 g of Echinacea purpurea, crush and pass through a 65-mesh sieve to obtain coarse powder of Echinacea purpurea; add ethanol solution with a volume ratio concentration of 30% to the coarse powder of Echinacea purpurea, and reflux extraction twice , add 15L for the first time, 8L for the second time, 30 minutes each time; the two extracts obtained are combined, and the filtrate is concentrated under reduced pressure to a clear paste with a relative density of 1.15-1.20 (75-80°C). Dried, crushed, that is.

Embodiment 2

[0025] Effects of Echinacea Extract on Growth and Body Condition of Immunosuppressed Rats

[0026] Trial Grouping and Treatment

[0027] SD rats, 30, with an adaptation period of more than 3 days, were randomly divided into 3 groups, namely, the blank control group (NC group), the model group (IS group), and the Echinacea purpurea extract group (EE-IS group), and the IS group And EE-IS group with 40mg / kg cyclophosphamide, intragastric administration every other day, a total of 3 times, NC group with the same amount of normal saline intragastric administration. After modeling, the EE-IS group was given 1 g / kg Echinacea purpurea extract by intragastric administration every day, and the other groups were given the same amount of normal saline, and were raised in the Experimental Animal Center of South China Agricultural University. Food, room temperature controlled at 20°C to 25°C, relative humidity at 30% to 70%, natural light.

[0028] Sample Collection

[0029] On the 14th ...

Embodiment 3

[0040] Effects of Echinacea Extract on Serum Biochemical and Cytokines in Immunosuppressed Rats

[0041] Test grouping and treatment are the same as in Example 2.

[0042] Sample Collection

[0043] On the 14th day after administration of Echinacea purpurea extract, 6 rats were randomly selected from each group for sampling, and fasted for 12 hours before sampling. Before blood collection, the weight of each rat was recorded, 2 mL of blood was extracted from the abdominal aorta, centrifuged at 3000 rpm / min for 10 min, and the obtained serum was used to measure serum biochemical and cytokine concentrations.

[0044] Detection and Analysis

[0045] Automatic biochemical analysis to detect serum glutamyl transpeptidase (GGT), total bilirubin (T-Bil), total protein (TP), blood urea nitrogen (UREA), alkaline phosphatase (ALP), aspartic acid Transaminase (AST), blood glucose (GLU), alanine aminotransferase (ALT), creatinine (CREA), total cholesterol (TC), triglyceride (TG), album...

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Abstract

The invention discloses an echinacea purpurea extract, which is prepared by the following steps: crushing echinacea purpurea, adding an ethanol solution with the volume ratio concentration of 20-50%, performing reflux extraction for more than two times, then combining extracting solutions, and performing concentrating first and then drying, thereby obtaining the echinacea purpurea extract. A preparation method of the echinacea purpurea extract and applications of the echinacea purpurea extract in the fields of regulating the intestinal content metabonomics of immunosuppressive rats and changing phosphoinositide metabolism, a phosphatidylinositol number system, ascorbic acid and aldose metabolism pathways of the immunosuppressive rats. The echinacea purpurea extract has the advantages that the echinacea purpurea extract has the effect of regulating immunosuppressive rat intestinal flora metabonomics, and animal experiments prove that the echinacea purpurea extract can improve the growth performance, and enhance the immune cell activity . The immune system is regulated by changing phosphoinositide metabolism, a phosphatidylinositol number system, ascorbic acid and aldose metabolism pathways.

Description

technical field [0001] The invention relates to the field of natural medicinal chemistry, in particular, an extract of Echinacea purpurea, its preparation method and its application in metabolomics, especially the application in changing the metabolic pathway of intestinal flora of immunosuppressed rats. Background technique [0002] The gut microbiota is an important environmental factor for human health and has evolutionarily protective roles in the body's metabolism, immunity, development, and behavior. In mammals, host-microbe symbiosis occurs primarily at mucosal surfaces, most importantly the intestinal mucosa. There are a large number of commensal microorganisms in the gut, their number is 10 times that of host somatic cells, and they have various metabolic and protective functions. The immune system is controlled by microorganisms. The intestinal flora regulates the metabolism of carbohydrates, lipids and amino acids, and plays an important role in maintaining the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P37/04A61P1/00A61P37/02
CPCA61K36/28A61P37/04A61P1/00A61P37/02A61K2236/333A61K2236/39A61K2236/51
Inventor 石达友杨嘉丽关钰澄刘翠郭世宁
Owner SOUTH CHINA AGRI UNIV
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