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Extraction method of China-hemp seed storage protein

A technology for protein storage and extraction method, which is applied to the protein composition of vegetable seeds, protein food ingredients, food science, etc., can solve problems such as poor effect, achieve obvious banding, reduce labor costs, and save fine separation and purification. effect of time

Pending Publication Date: 2021-09-21
HEILONGJIANG HEIKE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a method for extracting hemp seed storage protein in order to solve the problem that the existing hemp seed storage protein extraction method has poor effect

Method used

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  • Extraction method of China-hemp seed storage protein
  • Extraction method of China-hemp seed storage protein
  • Extraction method of China-hemp seed storage protein

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Experimental program
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specific Embodiment approach 1

[0013] Specific embodiment one: the extraction method of hemp seed storage protein in this embodiment is carried out according to the following steps:

[0014] 1. Extraction: Grind hemp seeds into powder, add n-hexane to the powder according to the weight ratio of hemp seeds at a ratio of 1:4, extract at room temperature for 3 hours to obtain the extract, and then use the weight ratio of hemp seeds Add n-hexane to the extract at a ratio of 1:6, dry the n-hexane after standing overnight to obtain defatted hemp powder;

[0015] 2. Add deionized water to the defatted hemp powder according to the ratio of parts by weight of 1:10, shake and extract at 37°C for 1 hour, place it at room temperature, and then centrifuge at 8000g for 30 minutes to obtain the above Supernatant A and Precipitation A, add deionized water to Precipitation A according to the weight ratio of 1:5, shake and leach at 37°C for 1 hour, then centrifuge at 8000g for 20 minutes at room temperature to obtain the sup...

specific Embodiment approach 2

[0017] Embodiment 2: This embodiment is different from Embodiment 1 in that: the hemp seeds in step 1 are ground into powder with liquid nitrogen. Other steps and parameters are the same as those in the first embodiment.

[0018] In this embodiment, fresh hemp seeds are ground into as fine a powder as possible with liquid nitrogen.

Embodiment 1

[0019] Embodiment 1 utilizes the method of the present invention to extract the storage protein of the different components in the hemp seed of Heilongjiang Province, and concrete steps are as follows:

[0020] 1. Extraction: Grind hemp seeds into powder, add n-hexane to the powder according to the weight ratio of hemp seeds at a ratio of 1:4, extract at room temperature for 3 hours to obtain the extract, and then use the weight ratio of hemp seeds Add n-hexane to the extract at a ratio of 1:6, dry the n-hexane after standing overnight to obtain defatted hemp powder;

[0021] 2. Add deionized water to the degreased hemp powder (the hemp seeds are ground into powder with liquid nitrogen) according to the ratio of parts by weight of 1:10, shake and extract at 37°C for 1 hour, then cool to room temperature, then Centrifuge at 8000g for 30 minutes to obtain supernatant A and precipitate A, add deionized water to precipitate A at a ratio of 1:5 by weight, shake and extract at 37°C ...

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Abstract

The invention discloses an extraction method of China-hemp seed storage protein, and relates to an extraction method of protein. The method solves the problem that an existing method for extracting the China-hemp seed storage protein is poor in effect. The extraction method of the China-hemp seed storage protein comprises the following steps: 1, extracting; 2, combining two centrifuged supernatants to obtain water-soluble protein; and 3, combining the two centrifuged supernatants to obtain salt-soluble protein. According to the method, the extraction of the China-hemp seed storage protein is realized, and the extraction effect is good. According to the extraction of the China-hemp seed storage protein, the China-hemp seed storage protein is primarily separated according to different solubility, then water-soluble protein and salt-soluble protein in China-hemp seeds are obtained respectively, the two proteins have different effects, and more scientific and more efficient proteins can be obtained according to actual requirements and different proportions. And the protein mixture has higher nutritional value.

Description

technical field [0001] The invention relates to a method for extracting stored protein. Background technique [0002] Hemp (industrial hemp) has been widely used as a fiber source for a long time. The earliest record can be traced back to more than 8000 BC, as hemp rope for pottery. About 2000 years ago, China invented papermaking in Cai Lun Before the craft, hemp was also used to make paper. [0003] In addition to fiber, in recent years, people have increasingly realized that hemp seeds are also a high-quality source rich in protein and oil. One of the more regions is China. Hemp contains 20-25% protein. Hemp seed storage protein is rich in 8 kinds of essential amino acids that cannot be synthesized by the human body. Its amino acid content is similar to soybean protein. Hemp seed storage The content of sulfur-containing amino acids in protein was significantly higher than that of soybean protein, and the content of sulfur-containing amino acids was proved to be related ...

Claims

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Application Information

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IPC IPC(8): A23J1/14
CPCA23J1/142
Inventor 孙鑫王雷孙尧李瑶吴琼
Owner HEILONGJIANG HEIKE TECH CO LTD
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