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Method for simultaneously detecting multiple cannabinoids in sample containing cannabis sativa leaf extract by utilizing high performance liquid chromatography

A technology of cannabis leaves and extracts, applied in the field of analytical chemistry, can solve the problems of few detection methods of cannabinoids, etc.

Pending Publication Date: 2021-09-14
CHINA NAT INST OF STANDARDIZATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few methods for the detection of cannabinoids in cosmetics, and there are even fewer studies on the simultaneous detection of multiple cannabinoids in cosmetics containing cannabis leaf extracts

Method used

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  • Method for simultaneously detecting multiple cannabinoids in sample containing cannabis sativa leaf extract by utilizing high performance liquid chromatography
  • Method for simultaneously detecting multiple cannabinoids in sample containing cannabis sativa leaf extract by utilizing high performance liquid chromatography
  • Method for simultaneously detecting multiple cannabinoids in sample containing cannabis sativa leaf extract by utilizing high performance liquid chromatography

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Embodiment 1

[0034] Detection method

[0035] 1.1. Introduction to Materials and Instruments:

[0036] Standard cannabinoids: CBDA, CBD, CBN, THC, THCA standard (1mg / mL, 1mL,) (Cerilliant, USA); methanol, acetonitrile (chromatographically pure) (Merck, Germany); formic acid (Sinopharm Chemical Reagent Co., Ltd. Company); 6 kinds of cosmetics containing cannabis leaf extract were purchased from Taobao.

[0037] iChrom5100 analytical domestic liquid chromatograph, equipped with DAD detector (Dalian Yilite Analytical Instrument Co., Ltd.); the technical scheme of the present invention can adopt C18 chromatographic column (that is, the strong hydrophobic packing of the silica gel matrix bonded in octadecyl group) , such as Supersil-ODS2 chromatographic column (4.6mm × 250mm, 5μm) (Dalian Elite Analytical Instrument Co., Ltd.); Thmorgan-VM200 vortex oscillator (Tuo Morgan Biotechnology Co., Ltd.); HITACHI-CF15RXII centrifuge (Hitachi Corporation ); sartorius analytical balance (Sartorius Scie...

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Abstract

The invention belongs to the field of analytical chemistry, and particularly relates to a method for simultaneously detecting various cannabinoids in a sample containing a cannabis sativa leaf extract by utilizing high performance liquid chromatography. The invention provides a method for simultaneously detecting various cannabinoids in a sample containing a cannabis sativa leaf extract by utilizing high performance liquid chromatography. A C18 chromatographic column is adopted, a formic acid aqueous solution is used as a mobile phase A, and formic acid acetonitrile is used as a mobile phase B to carry out gradient elution; the mass concentration of the formic acid aqueous solution is about 0.1-0.5%; the mass concentration of the formic acid acetonitrile is about 0.1%-0.5%; and the cannabinoid comprises one or more of tetrahydrocannabinol acid (THCA), cannabidiol acid (CBDA), cannabinol (CBN), cannabidiol (CBD) or tetrahydrocannabinol (THC). The method has the advantages of being accurate in quantification, simple and convenient to operate, stable and reliable.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for simultaneously detecting multiple cannabinoids in samples (eg, cosmetics) containing cannabis leaf extracts by using high performance liquid chromatography (HPLC). Background technique [0002] Industrial hemp refers to hemp industrialized planting varieties cultivated by biological breeding technology with tetrahydrocannabinol (THC) content lower than 0.3%. Industrial hemp cosmetics refer to cosmetics added with raw materials derived from industrial hemp plants, including industrial hemp seed oil, hemp leaf extract, etc. Internationally, industrial hemp cosmetics are generally classified as cannabidiol (CBD) cosmetics, mainly including cosmetics containing CBD or industrial hemp seed oil. With the legalization of industrial hemp, cosmetics containing hemp leaf extracts have developed rapidly. Since 2017, CBD, the main active ingredient in industrial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/36
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/36
Inventor 兰韬云振宇席兴军初侨于聪聪孟玲玲张文焕
Owner CHINA NAT INST OF STANDARDIZATION
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