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Method for detecting content of undecylenoyl phenylalanine

A technology of enoyl phenylalanine and a determination method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve problems such as poor reproducibility, and achieve the effects of good linearity, good recovery, high accuracy and sensitivity

Inactive Publication Date: 2021-09-10
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to overcome the defects and insufficiencies of the poor reproducibility of the existing assay methods for the determination of undecylphenylalanine, and provide a method for the assay of undecylylphenylalanine

Method used

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  • Method for detecting content of undecylenoyl phenylalanine
  • Method for detecting content of undecylenoyl phenylalanine
  • Method for detecting content of undecylenoyl phenylalanine

Examples

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Embodiment 1

[0052] A method for determining the content of undecyl phenylalanine, using high-performance liquid chromatography to detect the content of undecyl phenylalanine raw materials, the stationary phase is octadecylsilane bonded silica gel, and the mobile phase is It is chromatographically pure methanol (A)-phosphoric acid aqueous solution (B).

[0053] Wherein, the stationary phase is a YMC C18 chromatographic column (4.6×150mm, 3 μm); the volume fraction of phosphoric acid aqueous solution is 0.10%, isocratic elution (0~12min, 77%A, 23%B), detector: ultraviolet absorption light Detector; detection wavelength: 215nm; column temperature: 30°C; flow rate: 1.0mL / min; injection volume: 10μL. The number of theoretical plates is not less than 5000 based on the undecylenoyl phenylalanine peak.

[0054] Solution preparation:

[0055] (1) Preparation of reference substance solution: take about 10.00 mg of undecylenoyl phenylalanine reference substance (purity 99.81%), accurately weigh it...

Embodiment 2

[0059] A method for determining the content of undecyl phenylalanine, using high-performance liquid chromatography to detect the content of undecyl phenylalanine raw materials, the stationary phase is octadecylsilane bonded silica gel, and the mobile phase is It is methanol (A)-phosphoric acid aqueous solution (B).

[0060] Wherein, the stationary phase is a YMC C18 chromatographic column (4.6×150mm, 3 μm); the volume fraction of phosphoric acid aqueous solution is 0.20%, isocratic elution (0~12min, 80%A, 20%B), detector: ultraviolet absorption light Detector; detection wavelength: 215nm; column temperature: 30°C; flow rate: 1.0mL / min; injection volume: 10μL. The number of theoretical plates is not less than 5000 based on the undecylenoyl phenylalanine peak.

[0061] Preparation of solution

[0062] (1) Preparation of reference substance solution: take about 10.00 mg of undecylenoyl phenylalanine reference substance (purity 99.81%), accurately weigh it, put it in a 10mL meas...

Embodiment 3

[0066] A method for determining the content of undecyl phenylalanine, using high-performance liquid chromatography to detect the content of undecyl phenylalanine raw materials, the stationary phase is octadecylsilane bonded silica gel, and the mobile phase is It is methanol (A)-phosphoric acid aqueous solution (B).

[0067] Wherein, the stationary phase is a YMC C18 chromatographic column (4.6×150mm, 3 μm); the volume fraction of phosphoric acid aqueous solution is 0.03%, isocratic elution (0~12min, 75%A, 25%B), detector: ultraviolet absorption light Detector; column temperature: 30°C; detection wavelength: 215nm; flow rate: 1.0mL / min; injection volume: 10μL. The number of theoretical plates is not less than 5000 based on the undecylenoyl phenylalanine peak.

[0068] Solution preparation:

[0069] (1) Preparation of reference substance solution: take about 10.00 mg of undecylenoyl phenylalanine reference substance (purity 99.81%), accurately weigh it, put it in a 10mL volume...

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Abstract

The invention discloses a method for detecting the content of undecylenoyl phenylalanine, and relates to the technical field of phenylalanine content determination. The method for detecting the content of undecylenoyl phenylalanine adopts high performance liquid chromatography to detect the content of undecylenoyl phenylalanine, a stationary phase is octadecylsilane chemically bonded silica, and a mobile phase is a methanol-phosphoric acid aqueous solution. The mobile phase selects a methanol-phosphoric acid aqueous solution, the peak pattern and the separation degree of undecylenoyl phenylalanine are improved, the detection method is simple, convenient and easy to implement, high in accuracy and sensitivity and good in reproducibility, after verification of the method, the specificity, the linearity and the instrument precision are good, and under the detection method, a sample solution can be stably placed at room temperature, the recovery rate is good, and an effective detection method is provided for content detection of the undecylenoyl phenylalanine raw material.

Description

technical field [0001] The present invention relates to the technical field of content determination of phenylalanine, and more specifically, relates to a method for determining the content of undecylenyl phenylalanine. Background technique [0002] Undecylenyl phenylalanine is the lipid amino acid structure of phenylalanine. Its structure is similar to that of melanocyte stimulating hormone (α-MSH). It is an antagonist of α-MSH, mainly through competitive binding The melanin-stimulating hormone receptor MC1-R on melanocytes prevents melanocytes from producing tyrosinase, thereby inhibiting the activity of melanocytes and reducing the production of melanin. It can play a role in various stages of melanin formation triggered by α-MSH, intercept melanin production signals, control the combination of α-MSH and melanin production factors, inhibit the activity of tyrosinase, and then block the formation of melanin. This link comprehensively inhibits the production of melanin. ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/74G01N30/32G01N30/30G01N30/88G01N30/86
CPCG01N30/02G01N30/34G01N30/74G01N30/32G01N30/30G01N30/88G01N30/8634G01N2030/324G01N2030/884
Inventor 余楚钦汤文静唐永胡燕林华庆刘柔佳
Owner GUANGDONG PHARMA UNIV
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