Method for producing glutamic acid by using corynebacterium glutamicum

A technology of Corynebacterium glutamicum, glutamic acid, applied in microorganism-based methods, biochemical equipment and methods, adding compounds to stimulate growth, etc., can solve the problem of incomplete cell membrane structure, temperature sensitivity, glutamic acid accumulation And other issues

Pending Publication Date: 2021-09-10
HENAN UNIVERSITY OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the mutation of the gene controlling cell membrane synthesis of this species, it is sensitive to temperature. When the temperature increases, the growth of the bacteria will stop after doubling, and the cell membrane structure will be incomplete, resulting in a large accumulation of glutamic acid.

Method used

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  • Method for producing glutamic acid by using corynebacterium glutamicum
  • Method for producing glutamic acid by using corynebacterium glutamicum
  • Method for producing glutamic acid by using corynebacterium glutamicum

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Experimental program
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Effect test

Embodiment 1

[0080] Embodiment 1: the preparation of Corynebacterium glutamicum bacterial strain FM19-007 described in the application

[0081] The specific preparation process can be found in the application of Chinese Patent Publication No. CN111925953A.

Embodiment 2

[0082] Embodiment 2: Utilize the glutamic acid bacterial strain described in the application to produce glutamic acid

[0083] (1) Preparation of seed solution

[0084] Get the bacterium colony of the Corynebacterium glutamicum bacterial strain FM19-007 that the slant preserves and cultivates, adjust the thalline amount to be (1-3) * 10 6 1 / ml, prepare 0.2 ml of suspension solution, use it as the primary seed solution, shake the table at 100 times / min, and cultivate at 29°C until the OD value reaches about 0.8, which takes about 9 hours;

[0085] (2) Preparation of fermentation broth

[0086] Inoculate the seed solution in step (1) into the fermentation medium, the inoculation ratio is 10%, and carry out fermentation culture at 30°C. When the OD value reaches about 1.0, it takes about 1 hour, and different treatments are carried out according to different fermentation stages;

[0087] in particular:

[0088] Adaptation period (from OD value 1.0 to 4h): adjust the fermentati...

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Abstract

The invention relates to a method for producing glutamic acid by using corynebacterium glutamicum. According to the corynebacterium glutamicum, the strain is a corynebacterium glutamicum FM19-007 strain, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 18678. The method comprises the steps of preparing a seed solution and adding esculin in the fermentation process of corynebacterium glutamicum, so that glutamic acid is obtained at high yield.

Description

technical field [0001] The application belongs to the technical field of biological fermentation engineering, and in particular relates to a treatment method for interfering with the formation of bacterial film during the fermentation and acid production of Corynebacterium glutamicum. Background technique [0002] The cell membrane is the most important and one of the most basic structural components of biological cells. As an elastic semipermeable membrane mainly composed of phospholipids, its main function is to selectively exchange substances, absorb nutrients, and excrete metabolism. substances, secretion and transport of proteins, etc., and these functions are directly related to the formation density of phospholipid molecules. [0003] The structure and composition of the cell membrane have a great relationship with the function of the cell. Under normal physiological conditions, the structure of the cell membrane, surface receptors, ion channels, etc. maintain a relat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/18C12N1/20C12N1/38C12R1/15
CPCC12P13/18C12N1/20C12N1/38
Inventor 黄继红侯银臣廖爱美魏兆军董得平王晓曦杨玉岭潘龙臧立华杜丽平冯军伟
Owner HENAN UNIVERSITY OF TECHNOLOGY
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