Medicine composition for preventing and treating swine envelope virus infectious diseases, preparation method of medicine composition, and application of plant extract

A plant extract, infectious disease technology, application in medicine, pharmaceutical composition and preparation field for preventing porcine enveloped virus infectious disease, can solve problems such as unsatisfactory immune effect of vaccines and influence on protective effect

Inactive Publication Date: 2021-09-10
BIOFORTE BIOTECHSHENZHEN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For newborn piglets and lactating piglets, there are two problems in vaccine immunization: first, for sows whose serum is positive for PEDV antibodies, maternal antibodies in milk will affect the protective effect induced by oral live vaccines; second, PEDV vaccine It plays a role through mucosal immunity, but the high-incidence groups of PED are newborn piglets and lactating piglets with immature mucosal immune system, which leads to the unsatisfactory immune effect of PEDV vaccine
[0011] However, there are currently few additives or drug combinations specifically aimed at preventing and treating porcine enveloped virus-infected diseases

Method used

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  • Medicine composition for preventing and treating swine envelope virus infectious diseases, preparation method of medicine composition, and application of plant extract
  • Medicine composition for preventing and treating swine envelope virus infectious diseases, preparation method of medicine composition, and application of plant extract
  • Medicine composition for preventing and treating swine envelope virus infectious diseases, preparation method of medicine composition, and application of plant extract

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0058] Among the present invention, the preparation method of zinc-rich inactivated lactic acid bacteria bacterial agent is as follows: 1) Lactobacillus acidophilus carries out strain preparation earlier, and strain screening uses solid MRS medium as basic medium, adds 0.1% CaCO 3 , the pH value is 6.4, the medium is sterilized at 121°C for 20-30 minutes, cooled to 37°C, and set aside; 2) The fermentation culture of Lactobacillus acidophilus, the fermentation medium uses liquid MRS medium as the basic medium, The pH value is 6.4, the medium is sterilized at 121°C for 20-30 minutes, cooled to 37°C, and 0.12% ZnSO is added 4 , mix well, and set aside, the seed age is 12-18 hours, the inoculation amount is 3%-5% of V / V or V / W, the fermentation time is 36-48 hours, the fermentation temperature is 37°C, and the pH value is maintained at about 5.5; 3) Add 0.1-0.5% (w / v) of a protective agent to the fermentation broth after the fermentation is finished, and the protective agent is sk...

Embodiment 1

[0060] Example 1 Pelargonium extract inhibits PEDV test in vitro

[0061] 1.1 Toxicity test of geranium extract to Vero cells

[0062] Inoculate 6 × 10 in a 96-well plate 5 Cells / mL cell suspension (100 μL / well), leave a column without inoculating cells. At 37°C, 5% CO 2 cultured until the cells were fully confluent. The culture medium was aspirated, washed 3 times with PBS, and the geranium extract (hereinafter abbreviated as PE9) was diluted with DMEM to different concentrations, and added to a 96-well plate (100 μL / well), with 8 replicates for each dilution concentration. At 37°C, 5% CO 2 After culturing for 24h and 48h under certain conditions, the drug solution was sucked off, washed 3 times with PBS, and 10% CCK-8 solution was prepared in DMEM and added to a 96-well plate (100 μL / well). 2 Incubate for 1 h under the conditions, measure the absorbance (λ=450nm) with a microplate reader, and calculate the cell viability after 24 h and 48 h of treatment with different c...

Embodiment 2

[0084] Example 2 Fennel Extract Inhibits PEDV Proliferation in Vitro

[0085] 2.1 Toxicity test of fennel extract on Vero cells

[0086] The steps of the experimental method are as described in 1.1 of Example 1. The result is as Figure 4 As shown, through comparative experiments, when the concentration of fennel extract (abbreviated as A) is 1000 μg / mL or below, it has no significant effect on the viability of Vero cells.

[0087] 2.2. The test of fennel extract inhibiting the proliferation of PEDV in Vero cells

[0088] The steps of the experimental method are as described in 1.2 of Example 1. In this example, the fennel extract (A) was compared with other medicines. Two concentration gradients were taken at the highest safe concentration of each drug for 24 hours, and the drug was added during the whole process of PEDV proliferation. At the end of the first replication cycle of PEDV, that is, at 6h.p.i., RNA samples were harvested, and real-time quantitative fluorescent...

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Abstract

The invention relates to a medicine composition for preventing and treating swine envelope virus infectious diseases, a preparation method of the medicine composition, and application of a plant extract. The plant extract disclosed by the invention comprises anti-virus-replication natural plant extracts such as peucedanum praeruptorum dunn extracts, folium ginkgo epicarp extracts, radix saposhnikoviae extracts, Illicium verum extracts, Pelargonium hortorum extracts and the like, or is compounded with a proper antibacterial agent and auxiliary materials, and can be used for preventing and treating swine envelope virus infection and secondary infection caused by the swine envelope virus infection; and a third "defensive line" is built for the pig industry, and the defects of vaccine immunization and "decontamination" means are overcome. The medicine composition has an important practical significance for guaranteeing normal production of the pig industry, especially has the important practical significance for diseases caused by infection of the envelope virus which can not be prevented by a vaccine at present, or diseases with a low vaccine protection rate, or especially has the important practical significance by serving as a remedial measure for immune failure.

Description

technical field [0001] The invention relates to the application field of biotechnology, in particular to a pharmaceutical composition and preparation method for preventing and treating porcine enveloped virus infectious diseases, and the application of plant extracts in the preparation of medicines for preventing and treating porcine enveloped virus infectious diseases. Background technique [0002] Porcine epidemic diarrhea (Porcine epidemic diarrhea, PED) is an acute and highly contagious disease of pigs. Alphacoronavirus (KOCHERHANS R, BRIDGENA, ACKERMANN M, et al. 2001. Completion of the porcine epidemic diarrhea coronavirus (PEDV) genome sequence [J]. Virus Genes, 23(2): 137-144.). PEDV can cause diarrhea, dehydration, and vomiting in newborn piglets, and has a high mortality rate. In recent years, PED has been widely prevalent in the world, and outbreaks have occurred in China, the United States, South Korea, Canada and other countries, seriously endangering the devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/23A61K36/16A61K36/238A61K36/235A61K36/185A61P1/12A61P31/14A61K35/744
CPCA61K36/23A61K36/16A61K36/238A61K36/235A61K36/185A61K35/744A61P1/12A61P31/14A61K2300/00
Inventor 胡文锋曹永长刘元徐志超胡学生靳改改朱剑锋
Owner BIOFORTE BIOTECHSHENZHEN CO LTD
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