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Comparative transcriptome analysis method for peanut leaf gene differential expression under intercropping corn

A technology of transcriptome analysis and gene difference, applied in biochemical equipment and methods, sequence analysis, microbial measurement/inspection, etc., can solve problems such as lack of research, unexplored and analyzed, and impact on dwarf crop productivity, and achieve Method Accurate Effect

Active Publication Date: 2021-09-03
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Although the yield of the intercropping compound population of maize and peanuts with obvious plant height differences is significantly increased, the high-stalk crops of maize and short-stalk crops of peanuts will cause habitat stress and interspecific competition in space and time, and the resulting habitat stress is especially Low light stress will seriously affect the growth and development of dwarf crops, and lead to a serious impact on the productivity of dwarf crops
[0005] There have been relatively many reports on the impact of intercropping maize stress on peanut morphological development, photosynthetic characteristics and physiological characteristics, but little is known about the underlying mechanism of peanut response to intercropping maize stress, and peanut response to intercropping maize stress to regulate its There is also a lack of research on the mechanism of pod swelling, let alone the discovery and analysis of key differentially expressed genes and metabolic pathways in peanut functional leaf response to intercropping maize stress at the pod swelling stage using transcriptome sequencing

Method used

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  • Comparative transcriptome analysis method for peanut leaf gene differential expression under intercropping corn
  • Comparative transcriptome analysis method for peanut leaf gene differential expression under intercropping corn
  • Comparative transcriptome analysis method for peanut leaf gene differential expression under intercropping corn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Comparative Transcriptome Analysis of Peanut Functional Leaf Response to Intercropping Maize Stress at Pod Expansion Stage:

[0068] 1. Establishment of corn intercropping peanut system:

[0069] Peanut variety: Osmanthus 836; this variety is a peanut variety that is mainly planted in Guangxi for disease resistance, high yield and suitable for intercropping;

[0070] Corn variety: Guidan 0810; this variety is a high-yield corn variety in Guangxi;

[0071] Experimental address: Wuming Lijian Base, Guangxi Academy of Agricultural Sciences;

[0072] Peanut planting time: March 10, peanut pod expansion period sampling time: June 15.

[0073] Soil conditions: total nitrogen, total phosphorus and total potassium content are 0.113%, 0.053%, 0.24% respectively, hydrolyzable nitrogen 88mg / kg, available phosphorus 11.4mg / kg, available potassium 110mg / kg, organic matter 18.5g / kg; Red loam, PH value 7.1.

[0074] Experimental design: a randomized block design was adopted, with ...

Embodiment 2

[0145] qRT-PCR verification of differentially expressed genes

[0146] In order to verify the reliability of transcriptome sequencing, 17 differentially expressed genes (DEGs) were selected for real-time fluorescent quantitative PCR test, and the transcriptome sequencing results were compared according to Example 1, and 17 genes were selected to be related to carbon and nitrogen metabolism, amino acid metabolism, Differentially expressed genes related to lipid metabolism, sugar metabolism and chlorophyll metabolism as well as plant hormone signaling and plant-pathogen interactions, plus ribose phosphate-3-epimerase and glycerol-3-phosphate-2-O- For the two important genes of acyltransferase, Actin11 was selected as the internal reference gene. Design specific primers, the primer sequences are shown in Table 8:

[0147] Table 8 Primer pairs for important differentially expressed genes (see SEQ ID NO.1-SEQ ID NO.36 for details)

[0148]

[0149]

[0150] The method for a...

Embodiment 3

[0154] After designing the experiment according to the method of Example 1, in the peanut pod expansion stage, select 3 peanut plants of normal growth from each plot of peanut single cropping (contrast), and from each row of each plot of corn intercropping peanut (treatment) Select 3 normal growing peanut plants (18 plants in total), investigate and measure the plant height and lateral branch length of peanut plants under two planting modes of peanut single cropping and corn intercropping peanut, as well as peanut functional chlorophyll SPAD value and net photosynthetic rate, The chlorophyll SPAD value of the inverted three-leaf functional leaf of the peanut main stem was measured using a Japanese SPAD-502 chlorophyll meter, and the net photosynthetic rate Pn value of the inverted three-leaf functional leaf of the peanut main stem was measured using a CI-340 portable photosynthetic analyzer produced by CID Corporation of the United States Measured during the time period from 9:...

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Abstract

The invention relates to the technical field of biology, and particularly relates to a comparative transcriptome analysis method for peanut leaf gene differential expression under intercropping corn. The method comprises the steps that an RNA-seq technology is utilized for comparing and analyzing differential expression genes of peanut functional leaves in the pod expansion period under peanut single cropping and intercropping corn stress; differential genes related to metabolic pathways are taken as main genes, important genes and key metabolic pathways formed by peanut pod yield under intercropping corn stress are excavated, and a theoretical basis is provided for reducing adverse effects of intercropping corn stress on peanut yield by means of a directional chemical regulation and control technology; and the relative expression level of candidate differential expression genes is verified through qRT-PCR detection, and finally the qRT-PCR detection result is roughly consistent with multiple changes in transcriptome sequencing analysis. The transcriptome sequencing analysis method is accurate, feasible, effective and simple.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a comparative transcriptome analysis method for the differential expression of genes in peanut leaves under intercropping corn. 【Background technique】 [0002] Peanut is an important oil crop and economic crop in my country. Peanut intercropping is a green, high-efficiency and high-yield planting mode widely used in time, space and ecology, and the intercropping cultivation mode between different crops is very rich. Among them, the intercropping of corn and peanuts has incomparable advantages over traditional single cropping. The growth and development of corn and peanuts are complementary in time and space. Intercropping between the two can not only change the ecological structure of the farmland, but also make full use of light, temperature, water, heat and nutrients and other resources, improve the high-efficiency utilization ability of maize to strong light and peanut to low li...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6851C12N15/11G16B30/10G16B50/10
CPCC12Q1/6895C12Q1/6851G16B30/10G16B50/10C12Q2600/13C12Q2600/158C12Q2600/166C12Q2531/113C12Q2545/101
Inventor 熊发前刘俊仙刘菁唐荣华邹成林李忠韩柱强唐秀梅丘立杭吴海宁黄志鹏贺梁琼蒋菁钟瑞春
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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