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Marine bioactive peptide with remarkable skin sunburn protection effect as well as preparation method and application of marine bioactive peptide

A technology of marine organisms and active peptides, applied in the field of biomedicine, can solve problems such as difficult large-scale industrial production and application, low yield of active peptides, and complicated preparation methods, achieving short production cycles, simple preparation processes, and good physiological effects Effect

Active Publication Date: 2021-09-03
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the preparation methods involved are relatively complicated, coupled with the limitation of the sample processing capacity of high-precision separation techniques such as high-performance liquid chromatography, the yield of the final active peptide is low and the preparation cost is high, making it difficult to carry out large-scale industrial production and application.

Method used

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  • Marine bioactive peptide with remarkable skin sunburn protection effect as well as preparation method and application of marine bioactive peptide
  • Marine bioactive peptide with remarkable skin sunburn protection effect as well as preparation method and application of marine bioactive peptide
  • Marine bioactive peptide with remarkable skin sunburn protection effect as well as preparation method and application of marine bioactive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Take fresh and live clam scallops that meet the standards for the use of relevant seafood in my country, remove the shell, homogenize the soft body, add 4 times the volume of PBS buffer (0.01M, pH8.0), add trypsin (4000U / g shellfish), Enzymolysis was performed at a temperature of 37°C for 5 hours. The enzymolysis solution was boiled for 10 minutes and centrifuged at 10000×g for 10 minutes. The supernatant was ultrafiltered sequentially through ultrafiltration membranes with a molecular weight cut-off of 10 kDa and 3 kDa. Among them, the 3kDa-10kDa active peptide component is recorded as T1, and the active peptide component less than 3kDa is recorded as T2. Concentrate the T1 and T2 components to a concentration of about 200mg / mL, and then separate them with Sephadex G-15 (100×4.0cm): the sample volume is 8mL, the mobile phase is distilled water, the flow rate is 10mL / min, and the detection wavelength 220nm, 280nm.

[0066] The T1 (3kDa-10kDa) fraction was separated by...

Embodiment 2

[0072] Take fresh and live clam scallops that meet the standards for the use of relevant seafood in my country, remove the shell, homogenize the soft body, add 3 times the volume of deionized water, adjust the pH value to 7.5, add trypsin (3000U / g shellfish), and the temperature is 40 ℃, enzymatic hydrolysis for 4h. The enzymolysis solution was boiled for 10 minutes and centrifuged at 8000×g for 12 minutes. The supernatant was ultrafiltered sequentially through ultrafiltration membranes with a molecular weight cut-off of 10 kDa and 3 kDa. Among them, the 3kDa-10kDa active peptide component is recorded as T1, and the active peptide component less than 3kDa is recorded as T2. Concentrate the T1 and T2 components to a concentration of about 100mg / mL, and then separate them with Sephadex G-15 (100×4.0cm): the loading volume is 10mL, the mobile phase is distilled water, the flow rate is 10mL / min, and the detection wavelength 220nm, 280nm.

[0073] Separate the T1 (3kDa-10kDa) fra...

Embodiment 3

[0078] Take fresh and live clam scallops that meet the standards for the use of relevant seafood in my country, remove the shells, take the soft part of the homogenate, add 2 times the volume of distilled water, adjust the pH value to 7, add trypsin (2000U / g shellfish), and heat at a temperature of 50 ° C. , Enzymolysis 6h. The enzymolysis solution was boiled for 10 minutes and centrifuged at 6000×g for 15 minutes. The supernatant was ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 10 kDa, and the fraction of the filtrate (active peptide less than 10 kDa) was recorded as T. Concentrate the T component to a concentration of about 150mg / mL, and then separate it with Sephadex G-15 (100×4.0cm): sample volume is 12mL, mobile phase is distilled water, flow rate is 10mL / min, detection wavelength is 220nm, 280nm .

[0079] After the T (less than 10kDa) fraction is separated by Sephadex G-15, the first to sixth elution peak fractions are collected se...

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Abstract

The invention provides a marine bioactive peptide with a remarkable skin sunburn protection effect as well as a preparation method and application of the marine bioactive peptide. The marine bioactive peptide is small in molecular weight, good in skin compatibility, and capable of remarkably reducing apoptosis and necrosis of cells irradiated by ultraviolet rays and reducing dermal inflammatory exudation and elastic fiber degradation. The preparation method of the marine bioactive peptide comprises the following steps: taking a soft part of a fresh marine shellfish, carrying out protease enzymolysis, ultrafiltration and sephadex separation, discarding the last elution single peak, respectively collecting or mixing other elution components, and freeze-drying to obtain the marine bioactive peptide. The method is simple, low in cost, green, environment-friendly, short in production cycle and suitable for industrial production, the prepared active peptide has the effects of preventing and repairing skin sunburn, and can be applied to cosmetics and biomedical materials, the cosmetic dosage forms include cream, emulsion, gel, jelly, water aqua, mist spray and the like, and dosage forms of the biomedical materials comprise lotion, solution, dressing, ointment, injection and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a marine biologically active peptide with a significant skin sunburn protection effect and a preparation method and application thereof. Background technique [0002] With the destruction of the ozone layer, the skin is exposed to excessive ultraviolet rays for a long time, leading to an increase in the incidence of actinic skin diseases, basal cell carcinoma, and squamous cell carcinoma. The formation and occurrence of skin cancer induced by ultraviolet rays is a complex and continuous biological behavior, in which ultraviolet rays (UVB) with a wavelength of 280-320nm are located near the absorption peaks of DNA and proteins, can cause DNA and protein damage, and are the most carcinogenic or mutation. The DNA damage of epidermal keratinocytes by ultraviolet rays is the basis for inducing skin cancer. If the damaged DNA undergoes unrestricted repairs, it will cau...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/435C07K1/34C07K1/16C07K1/36A61K8/64A61K38/01A61Q17/04A61Q19/00A61P17/00
CPCC12P21/06C07K14/43504A61K8/64A61K38/012A61Q17/04A61Q19/004A61P17/00A61K2800/805
Inventor 陈华孙恢礼潘剑宇万鹏蔡冰娜
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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