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Application of schistosoma japonicum cysteine protease inhibitor to aspect of atherosclerosis

A technology of cysteine ​​protease and atherosclerosis, applied in the field of medicinal chemistry, can solve problems that have not been studied by anyone, and achieve the effect of improving the quality of life, good treatment effect, and promoting the healthy development of the population

Active Publication Date: 2021-09-03
BENGBU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that schistosome Cystatin can treat arthritis, sepsis, and colitis, but whether schistosome Cystatin can prevent, alleviate or treat atherosclerosis and its complications has not yet been studied

Method used

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  • Application of schistosoma japonicum cysteine protease inhibitor to aspect of atherosclerosis
  • Application of schistosoma japonicum cysteine protease inhibitor to aspect of atherosclerosis
  • Application of schistosoma japonicum cysteine protease inhibitor to aspect of atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] Preparation and purification of rSj-Cys:

[0031] (1) Prokaryotic expression of rSj-Cys

[0032] rSj- Full-length DNA in Cys. Add 10ml LB medium and kanamycin to 20μl E.coli BL21 target strain, shake the bacteria overnight at 37°C. Take 8ml of the overnight cultured bacterial solution and add it to 400ml LB medium containing kanamycin, shake the bacteria for 3 hours at 37°C, monitor the OD600 value of the bacterial solution to reach between 0.6-0.8, add 1mM isopropyl-β-D- For thiogalactoside (IPTG), continue to shake the bacteria and induce expression for 5 hours, collect the bacterial liquid and centrifuge, discard the supernatant to collect the precipitate, fully resuspend the precipitate with non-denaturing lysate, and add the protease inhibitor phenylmethylsulfonyl at the same time Fluorine (PMSF). Bacteria were fully disrupted by ultrasonication, and the bacterial disrupted solution was centrifuged to collect the supernatant, and 10 μl of the supernatant was ta...

Embodiment 1

[0037] Example 1. Effects of rSj-Cys on body weight change and kidney index in atherosclerotic mice

[0038] (1) Experimental materials:

[0039] Animal: Male apoE - / -Mice (C57BL / 6J background, 7-8 weeks old, SPF grade, body weight about 22 g) were purchased from the Experimental Animal Center of Bengbu Medical College. The mice were kept in a common animal room, free to eat and drink, and the light was controlled regularly. The animal experiment operation process was approved by the ethics committee and complied with the animal ethics standard (LAEC-2014-039).

[0040] Feed: high-fat feed (HFD: D12108C: 20% fat, 1.25% cholesterol, Nanjing Synergy Biotechnology Co., Ltd.); normal feed (NCD, Nanjing Synergy Biotechnology Co., Ltd.).

[0041] (2) Experimental method:

[0042] The experiment was divided into 4 groups, 6 mice in each group, respectively: apoE - / - +NCD group: fed normal diet NCD, injected with PBS; apoE - / - +NCD+rSj-Cys group: fed normal diet NCD, injected wi...

Embodiment 2

[0047] Example 2. Effects of rSj-Cys on Lipid Metabolism and Renal Function in Atherosclerotic Mice

[0048] (1) Experimental materials:

[0049] Sample: the mouse serum collected in Example 1.

[0050] Materials: Automatic Biochemical Analyzer (Beckman Coulter Company).

[0051] (2) Experimental method:

[0052] The mouse serum collected in Example 1 was detected on a full-automatic biochemical analyzer for total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL-c), high-density lipoprotein (HDL-c), creatinine (Cr) and blood urea nitrogen (BUN) levels.

[0053] (3) Experimental results:

[0054] The results showed that, with apoE - / - +NCD group compared with apoE - / - The levels of TC, TG, LDL-c, Cr and BUN in the +HFD group were significantly increased, and the level of HDL-c was significantly decreased; - / - Compared with the +HFD group, the levels of TC, TG, LDL-c, Cr and BUN were significantly decreased, and the level of HDL-c was significantly increa...

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Abstract

The invention discloses an application of a schistosoma japonicum cysteine protease inhibitor to the aspect of atherosclerosis, and belongs to the technical field of medicinal chemistry. The invention provides a medicine capable of preventing, relieving or treating atherosclerosis, and the effective component of the medicine is a schistosoma japonicum cysteine protease inhibitor Cystatin (rSj-Cys for short). The rSj-Cys can effectively relieve the atherosclerosis, reduce the serum lipid level, delay the inflammatory response of atherosclerotic mice, stabilize plaque development and reverse visceral pathological damage, and a new thought is provided for clinical treatment of the atherosclerosis.

Description

technical field [0001] The invention relates to the application of a cysteine ​​protease inhibitor of Schistosoma japonicum in atherosclerosis, and belongs to the technical field of medicinal chemistry. Background technique [0002] Atherosclerosis (Atherosclerosis, AS) is a progressive chronic inflammatory response, mainly involving large and medium-sized arteries. Lesions are characterized by lipid deposition in the arterial intima, leading to the formation of atherosclerotic plaques in the arterial intima, resulting in hardening and narrowing of blood vessels and tissue ischemia, causing a series of complications and seriously threatening human health. At present, the research on the pathogenesis of atherosclerosis is not yet clear. With the improvement of human living standards, obesity, hypertension, and hyperlipidemia are all risk factors for atherosclerosis. It is generally believed that abnormal lipid metabolism, chronic inflammation, oxidative stress, and immune dy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/57A61P9/10
CPCA61K38/57A61P9/10Y02A50/30
Inventor 杨小迪杨慧娟褚亮李徽徽陈兴智陈卫东
Owner BENGBU MEDICAL COLLEGE
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