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Cell culture paper as well as preparation method and application thereof

A cell culture and cell culture technology, applied in cell culture support/coating, biochemical equipment and methods, special paper, etc., can solve the problems of small pore size of cellulose membrane, complicated operation, difficult to meet the cell proliferation space, etc. Achieve the effect of small pore size and good water dispersibility

Pending Publication Date: 2021-08-27
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cellulose porous membrane prepared by electrospinning has an average pore size of about 80-1100nm, but the cellulose membrane prepared by these two methods has a small pore size, which is difficult to meet the space required for cell proliferation.
Later, cellulose membranes were prepared by combining dissolving regenerated cellulose and template method, adding a porogen during the regeneration process, and by controlling the size of the porogen, cellulose membranes with different pore diameters and porosity were prepared, but the operation was complicated , and the addition of organic solvents will be detrimental to cell growth

Method used

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  • Cell culture paper as well as preparation method and application thereof
  • Cell culture paper as well as preparation method and application thereof
  • Cell culture paper as well as preparation method and application thereof

Examples

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preparation example Construction

[0028] A method for preparing cell culture paper with adjustable pores of the present invention, specifically comprises the following steps:

[0029] Step 1, the preparation of carboxylated nanocellulose by Tempo oxidation method:

[0030] The cotton fiber after beating is flailed with the concentration of 1% by mass fraction in the standard fiber deflatterer, makes it evenly dispersed, adds the tetramethyl piperidine oxide (TEMPO ) solution and a NaBr solution with a mass fraction of 10%, were mixed and stirred for 10 minutes, and NaClO solution was added dropwise to make the pH of the suspension 10-12, and the whole process lasted for 2.5-3.5 hours. After the reaction is completed, the suspension is washed with deionized water until it becomes neutral to form a carboxylated nanocellulose solution, and deionized water is added to adjust the mass fraction of the carboxylated nanocellulose solution to 0.2% to 1.0%.

[0031] Step 2: Soak the qualitative filter paper with ethano...

Embodiment 1

[0036] A method for preparing cell culture paper with adjustable pores of the present invention, specifically comprises the following steps:

[0037] Step 1) Tempo oxidation method prepares carboxylated nanocellulose:

[0038] The cotton fibers after beating were decomposed with a mass fraction of 1% in a standard fiber de-fiberizer to make them evenly dispersed, and then added 1.6% TEMPO solution and 10% TEMPO solution dissolved in deionized water respectively. NaBr solution was mixed and stirred for 10 min, and NaClO solution was added dropwise to control the pH of the suspension at 11, and the whole process continued for 3 h.

[0039] After the reaction is completed, the suspension is washed with deionized water until it becomes neutral, and deionized water is added to adjust the mass fraction of the carboxylated nanocellulose solution to 0.2%.

[0040] Step 2) Soak the qualitative filter paper with 1:1 (volume fraction) ethanol aqueous solution for 5 minutes to pretreat t...

Embodiment 2

[0045] A method for preparing cell culture paper with adjustable pores of the present invention, specifically comprises the following steps:

[0046] Step 1) Tempo oxidation method prepares carboxylated nanocellulose:

[0047] The cotton fibers after beating were decomposed with a mass fraction of 1% in a standard fiber de-fiberizer to make them evenly dispersed, and then added 1.6% TEMPO solution and 10% TEMPO solution dissolved in deionized water respectively. NaBr solution was mixed and stirred for 10 min, and NaClO solution was added dropwise to control the pH of the suspension at 11, and the whole process continued for 3 h.

[0048] After the reaction is completed, the suspension is washed with deionized water until it is neutral, and deionized water is added to adjust the mass fraction of the carboxylated nanocellulose solution to 0.6%.

[0049] Step 2) Soak the qualitative filter paper with 1:1 (volume fraction) ethanol aqueous solution for 5 minutes to pretreat the fi...

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Abstract

The invention discloses cell culture paper and a preparation method and application thereof, and relates to the field of biopolymer materials, and the method comprises the following steps: immersing filter paper soaked in an alcohol solvent in a carboxylated nanocellulose solution with the mass fraction of 0.2-1.0%, and then naturally airing to obtain the cell culture paper. A method for culturing cells by adopting the cell culture paper is characterized by comprising the following steps: sterilizing the cell culture paper at 120-140 DEG C for 20-30 minutes, then inoculating a cell suspension of cells to be cultured onto the sterilized cell culture paper, and finally putting into a culture medium for culturing. Due to the existence of a large number of carboxyl groups on the surface of the carboxylated nano-cellulose, the carboxylated nano-cellulose has good water dispersibility, is small in aperture, can uniformly enter macropores of the filter paper and can adjust the pores to a range suitable for cell growth, and the obtained material is suitable for cell growth in porosity, good in mechanical property and capable of being used for cell culture.

Description

technical field [0001] The invention relates to the field of biopolymer materials, in particular to a cell culture paper and its preparation method and application. Background technique [0002] Paper has attracted a lot of attention as an emerging cell culture platform. Compared with other materials, paper is a cheaper and more abundant material, and has some special advantages, such as: (1) The raw material is cellulose or cellulose-polymer mixture, which has good biocompatibility; (2) It has a large number of pores, which can simulate the structure of extracellular matrix, allow substances to pass through, provide nutrients for cells and discharge metabolic waste in time; (3) facilitate chemical modification, realize the immobilization of biomolecules, and facilitate the deposition of various (4) The structure and thickness of the paper can be flexibly adjusted; (5) Multiple paper-based units can be easily stacked to achieve 3D structural tissue / organ construction, and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D21H27/00D21H27/08D21H23/22D21H25/06C12N5/09
CPCD21H27/00D21H27/08D21H23/22D21H25/06C12N5/0693C12N5/0631C12N5/0682C12N2533/78C12N2533/54
Inventor 张素风李磊刘亚丽唐蕊华林瑞李敏
Owner SHAANXI UNIV OF SCI & TECH
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