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Nanovesicles derived from bacteria of genus deinococcus, and use thereof

A deinococcus, vesicle technology, used in the prevention, composition of inflammatory diseases or neurological diseases, alleviation or treatment of cancer, can solve the problems of diagnosis and treatment of vesicles without Deinococcus bacteria, and achieves Injury-suppressing effect

Pending Publication Date: 2021-08-20
MD HEALTHCARE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, there have been no cases where vesicles derived from Deinococcus bacteria have been applied to the diagnosis and treatment of intractable diseases such as cancer, inflammatory diseases, and dementia

Method used

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  • Nanovesicles derived from bacteria of genus deinococcus, and use thereof
  • Nanovesicles derived from bacteria of genus deinococcus, and use thereof
  • Nanovesicles derived from bacteria of genus deinococcus, and use thereof

Examples

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Embodiment 9

[0095] Hereinafter, preferred embodiments to facilitate understanding of the present invention will be presented. However, the following examples are provided only for easier understanding of the present invention, and the content of the present invention is not limited by the following examples.

Embodiment 1

[0097] Example 1. Analysis of in vivo absorption, distribution and excretion patterns of bacteria and vesicles derived from bacteria

[0098]To assess whether bacteria and bacteria-derived vesicles are absorbed systemically through the gastrointestinal tract, the following experiments were performed. First, a dose of 50 μg of each of fluorescently labeled bacteria and bacteria-derived vesicles was gastrointestinally administered to the stomach of mice, and the Fluorescence was then measured. As a result of observing the whole image of the mouse, as Figure 1A As shown, the bacteria were not absorbed systemically, but vesicles derived from the bacteria were absorbed systemically at 5 minutes after administration, and strong fluorescence was observed in the bladder 3 hours after administration, making it possible to see that the vesicles were excreted into the urine road. Furthermore, it can be seen that the vesicles are present in vivo until 12 hours after administration (s...

Embodiment 2

[0100] Example 2. Evaluation of whether bacteria and vesicles derived from bacteria penetrate the protective membrane of the intestinal mucosa

[0101] To assess whether bacteria and bacteria-derived vesicles that penetrate the protective membrane of the mucosa infiltrate into tissues after direct administration of bacteria and bacteria-derived vesicles to the intestine, the transmucosal Infiltration into the intestinal tissue behind the protective membrane. To assess the presence of bacteria and vesicles in mucosal tissues, antibodies against bacteria and vesicles were prepared, attached to green fluorescent protein (GFP) and used, and in combination with 4,6-diamidino-2-phenylindole ( DAPI) staining and observed under a microscope.

[0102] As a result, it was confirmed that bacteria cannot penetrate the protective membrane of the mucosa, however, vesicles derived from bacteria infiltrate into the intestinal tissue through the mucosa (see figure 2 ).

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PUM

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Abstract

The present invention relates to vesicles derived from bacteria of the genus Deinococcus, and a use thereof. The present inventors experimentally ascertained that the vesicles in clinical samples of cancer, inflammatory disease and dementia patients are significantly more reduced than those in normal people, the secretion of inflammatory mediators by pathogenic vesicles such as E. coli-derived vesicles is significantly inhibited when the vesicles isolated from the strain are administered, and vesicles derived from bacteria of the genus Deinococcus significantly inhibit cranial nerve cell damage caused by stress hormones, and thus it is expected that vesicles derived from bacteria of the genus Deinococcus, according to the present invention, can be effectively used for the purpose of developing a method for diagnosing cancer, inflammatory diseases and / or dementia and a composition for preventing, alleviating or treating cancer, inflammatory diseases and / or cranial nerve diseases.

Description

technical field [0001] The present invention relates to nanovesicles derived from bacteria of the genus Rhodococcus (genus Rhodococcus) and uses thereof, and more specifically, to methods for diagnosing cancer, inflammatory diseases, dementia, etc., using nanovesicles derived from bacteria of the genus Rhodococcus, And a composition for preventing, alleviating or treating cancer, inflammatory diseases or cranial nerve diseases, comprising the vesicles. [0002] This application claims priority to Korean Patent Application No. 10-2019-0002608 filed on January 9, 2019 and Korean Patent Application No. 10-2020-0002267 filed on January 7, 2020 and the descriptions in these applications All disclosures in and in the accompanying drawings are incorporated into this application. Background technique [0003] Since the early 21st century, acute infectious diseases, which in the past were considered epidemics, have become less important, while chronic inflammatory diseases accompani...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6883C12Q1/6886C12Q1/6851A23L33/135A61K8/99A61K35/74A61P25/24A61P29/00A61P35/00
CPCA23L33/135A61K8/99A61K35/74A61P25/24A61P29/00A61P35/00C12Q1/6883C12Q1/6886C12Q1/689C12Q2600/156A61Q7/00A61Q19/00A61K8/14Y02A50/30C12Q1/6851
Inventor 金润根
Owner MD HEALTHCARE INC
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