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Luteolin with specific in-vitro anti-pseudorabies virus activity

A technique for pseudorabies virus and luteolin, which is applied in the direction of determination/inspection of antiviral agents, organic active ingredients, microorganisms, etc., and achieves the effects of simple operation, convenient use and obvious effect.

Inactive Publication Date: 2021-08-13
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to overcome the problem that there is no anti-pseudorabies virus drug at present, the present invention provides luteolin with specific external anti-pseudorabies virus activity, and this kind of luteolin with specific external anti-pseudorabies virus activity is mainly evaluated by in vitro experiments. Anti-Pseudorabies virus activity, experiments to verify whether luteolin can resist pseudorabies virus by inhibiting the replication of Pseudorabies virus, aiming to solve the problem

Method used

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  • Luteolin with specific in-vitro anti-pseudorabies virus activity
  • Luteolin with specific in-vitro anti-pseudorabies virus activity
  • Luteolin with specific in-vitro anti-pseudorabies virus activity

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Embodiment 1

[0068] The specific anti-pseudorabies virus activity of the present invention is luteolin, the luteolin used was purchased from Meilun Biology, with a purity of 98%; the used pseudorabies virus TJ strain (PRV) was purchased in Harbin, China in February 2021 Veterinary Institute; the composition of the cell growth medium used is: 89% DMEM medium, 10% fetal bovine serum and 1% double antibody are prepared in a sterile environment and stored in a refrigerator at 4°C; the cell maintenance medium used It consists of: 97% DMEM medium, 2% fetal calf serum and 1% double antibody are configured in a sterile environment and stored in a refrigerator at 4°C; the probes used in FQ-PCR in the examples of the present invention ( The probe 5' has a fluorescent group, and the 3' has a fluorescent quenching group) and primers as shown in Table 1; 50 ) and the specific requirements of the experiment are diluted with cell maintenance solution; the instruments used in the embodiments of the presen...

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Abstract

The invention relates to luteolin with specific in-vitro anti-pseudorabies virus activity, belongs to the technical field of preventive veterinary medicine, and is characterized in that 20 mu g / mL luteolin is used as a raw material of a drug capable of effectively inhibiting the reproduction of PRV in porcine kidney cells PK-15 on the porcine kidney cells PK-15 cultured in vitro within 48 hours; the luteolin has effects on replication, adsorption and penetration stages of virus infection; the inhibition effect on the replication stage is most obvious; when the concentration of the luteolin is 20 mu g / mL, the luteolin has no inactivation effect on viruses and has no protection effect on cells; when the luteolin is added in the virus replication stage, virus replication is inhibited by 10 times; when the concentration of the luteolin is below 20 mu g / mL, the inhibition rate of the luteolin to viruses reaches 80%; the antiviral activity is dose-dependent; the median inhibitory concentration IC50 to PRV is 6.775 mu g / mL; and the therapeutic index of the luteolin is 3.1542.

Description

technical field [0001] The invention relates to luteolin with specific anti-pseudorabies virus activity and belongs to the technical field of preventive veterinary medicine. Background technique [0002] Luteolin (luteolin) is a kind of natural flavonoids, which exists in many kinds of plants. It has a variety of pharmacological activities, such as anti-inflammatory, anti-allergic, uric acid-lowering, anti-tumor, anti-bacterial, anti-viral, etc. It is mainly used clinically for relieving cough, expectorant, anti-inflammatory, lowering uric acid, treating cardiovascular diseases, and treating "amyotrophic spinal cord side cable sclerosis", SARS, hepatitis, etc. Luteolin mostly exists in a variety of plants in the form of glycosides, and these plants have higher content in whole-leaf green orchid, pepper, wild chrysanthemum, honeysuckle, and perilla. Has antitussive and expectorant effects. According to the latest research, respiratory symptoms such as cough, expectoration,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P31/22C12Q1/70
CPCA61K31/352A61P31/22C12Q1/70
Inventor 朱妍门晓钰蔡晓静
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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