Method for detoxifying colocasia esculenta by droplet vitrification ultra-low-temperature therapy

Abstract

The invention discloses a method for detoxifying colocasia esculenta by droplet vitrification ultra-low-temperature therapy, and belongs to the technical field of agricultural production. The method comprises the following steps: pretreating stem tips of the colocasia esculenta by a gradient heating method; taking 0.5-1.5 cm of stem tips, transferring the stem tips to a basic culture medium, and performing illumination culture for 1-3 days; transferring the stem tips into a sugar-containing culture medium, loading for 20-30 min, performing dehydrating for 10-20 min by using PVS2, and preserving the stem tips for 1-2 days in liquid nitrogen; after unfreezing, washing the stem tips with a washing solution, then transferring the stem tips into a solid culture medium for dark culture for 2-3 days, and then conducting culture for 20-30 days under an illumination condition to differentiate new buds; and transferring the new buds into a solid culture medium for culturing to obtain virus-free seedlings of the colocasia esculenta. According to the method, after the stem tips of the colocasia esculenta are treated through gradient heating and an embedding medium, primary detoxification can be achieved, the high differentiation capacity of stem tip cells is kept, further detoxification is achieved in combination with a droplet vitrification method and ultralow-temperature treatment, and the detoxification rate and the survival rate of virus-free seedlings can be increased.

Description

technical field [0001] The invention relates to the field of agricultural production, in particular to a detoxification method of red bud taro droplet vitrification ultra-low temperature therapy. Background technique [0002] Red bud taro is a traditional superior product in Qianshan County, Jiangxi Province, with a long history of planting. With the rapid development of this industry, planting red-bud taro has become the main source of income for farmers in Yanshan County, and Qianshan County has also become the largest production base of red-bud taro in Jiangxi Province. In March 2007, it was approved by China Green Food Committee and The Ministry of Agriculture named it "National Green Food Standardized Production Base County of Red Bud Taro". It is precisely because of the rapid development of red bud taro in recent years, the long-term use of asexual reproduction to obtain seedlings in cultivation and planting has led to the accumulation of viruses, the degradation of ...

AI Technical Summary

Problems solved by technology

At present, virus-free seedlings are mainly detoxified through high-temperature detoxification method, stem tip culture detoxification method, shoot tip micro-grafting technology, heat treatment combined with shoot tip detoxification method, etc. Although these methods can also achieve detoxification to a certain extent, However, there are problems such as low detoxification efficiency and low survival rate of detoxified seedlings. In recent years, ultra-low temperature detoxification has also been continuously applied. For example, Professor Wang QC and his team successfully removed sweet potato virus by ultra-low temperature preservation; Luan Aiping found that, Cucumber Mosaic Virus (CMY) and Tobacco Mosaic Virus (TMV) can be completely removed from gerbera 2.0mm stem tip cryopreservation

At present, there are also relevant reports on the detoxification of red bud taro by ultra-low temperature method, but the detoxification rate of red bud taro is only about 45% by using the simple ultra-low temperature method, and there are virus-free seedlings due to incomplete detoxification. The problem of low cultivation survival rate is far from solving the fundamental problem for Qianshan County, which relies on red bud taro planting as its main source of income