Alcalibacterium water producing as1 and its application in sewage treatment

A technology for the production of Alcalibacterium, AS1, applied in biological water/sewage treatment, water/sludge/sewage treatment, water pollutants, etc., to achieve the effect of simplifying the denitrification process, wide pH adaptability, and rapid denitrification treatment

Active Publication Date: 2022-06-14
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biological denitrification process includes two processes of aerobic autotrophic nitrification and anaerobic heterotrophic denitrification, but it has the following disadvantages: (1) Autotrophic nitrifying bacteria grow slowly, have poor environmental adaptability, weak impact load resistance, and are easily destroyed by high Concentration of ammonia nitrogen and nitrite nitrogen inhibition
(2) The two reactions of nitrification and denitrification cannot be unified in time and space, which increases investment and operation costs
However, the suitable growth conditions for most strains isolated at present are relatively harsh, and the denitrification application for some special types of wastewater (such as acid-base wastewater, high-salt wastewater, and high-ammonia-nitrogen wastewater) is limited. The removal of high-concentration ammonia nitrogen and the high removal rate of COD and ammonia nitrogen in swine wastewater have not been reported

Method used

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  • Alcalibacterium water producing as1 and its application in sewage treatment
  • Alcalibacterium water producing as1 and its application in sewage treatment
  • Alcalibacterium water producing as1 and its application in sewage treatment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation and identification of strains:

[0024] Enrichment culture: The sample is the aerobic activated sludge collected by Cao Xianhe from a sewage treatment plant in Beijing in April 2020. Take 20 mL of fully mixed activated sludge and suspend it in 180 mL of 0.2% sodium chloride solution. 5 mL of the suspension was placed in a 250 mL Erlenmeyer flask filled with 100 mL of heterotrophic nitrification medium, and acclimatized and enriched at 30°C and 180 rpm. Acclimatization takes 48 h as a cycle. After the end of each cycle, 10 mL of the enrichment solution was added to a new 100 mL heterotrophic nitrification medium to continue the enrichment culture, so that the enrichment culture continued for 5 cycles. During this period, the removal of ammonia nitrogen in the culture medium was detected.

[0025] Take the culture solution after enrichment for 5 generations, and use sterile water according to 10 -3 -10 -7 100 µL dilutions were applied to heterotrophic nitrif...

Embodiment 2

[0036] Growth and heterotrophic nitrification characteristics of strain AS1:

[0037] After the strain AS1 was activated and cultured in the LB liquid test tube for 24 h, a certain amount of bacterial liquid was drawn according to the inoculum volume (v / v) of 2%, and the bacterial cells were collected by centrifugation at 4000 rpm for 5 min, and washed with sterile water. Inoculate in a 150 mL Erlenmeyer flask filled with 50 mL of heterotrophic nitrification medium (initial ammonia nitrogen concentration 100 mg / L, C / N=20), and culture in a shaker at 30°C and 180 rpm for 72 h, during which time Take a sample at 4 h to measure the OD600 value, and centrifuge the sample at 10,000 rpm for 10 min to remove bacteria, then take the supernatant to detect COD and NH in the water sample 4 -N, NO 2 -N, NO 3 -N, TN content.

[0038] The results are as follows and figure 1shown. The strain began to grow 4 hours after inoculation, and the growth of the strain basically ended after 24 ...

Embodiment 3

[0041] Effect of pH on growth and denitrification of strain AS1:

[0042] Adjust the pH of the heterotrophic nitrification medium to 3, 4, 5, 7, 8.5, 9.5, 10.5, 11.5, activate the strain AS1 according to the conventional method, and absorb a certain amount of The bacterial solution was centrifuged at 4000 rpm for 5 min to collect the bacterial cells, washed with sterile water, inoculated in the heterotrophic nitrification medium after pH adjustment, and cultured in a shaker at 30 °C and 180 rpm for 72 h, and the final results were detected. OD600 value under pH conditions, and the sample was centrifuged at 10000 rpm for 10 min to remove bacteria, then the supernatant was taken to detect COD and NH in the water sample 4 -N content.

[0043] The results are as follows and figure 2 shown. Strain AS1 can grow well and carry out heterotrophic nitrification in the pH range of 7-10.5, and the removal rate of ammonia nitrogen is between 92%-95%, and the removal rate of COD is betw...

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Abstract

The invention belongs to the technical field of microorganisms, in particular to the technical field of microbial wastewater treatment. The invention discloses a strain of water producing Alcaligenes aquatilis AS1, and its preservation number is CGMCC No.21282. The strain was deposited on December 2, 2020 at the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, referred to as CGMCC, at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. The strain can realize simultaneous removal of ammonia nitrogen, total nitrogen and COD under aerobic conditions.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to the technical field of microbial wastewater treatment. Background technique [0002] The main pollutants in my country's fresh water resources (including rivers, lakes, large-scale water conservancy projects and other surface water and groundwater) that have been detected to exceed the standard include chemical oxygen demand (COD), ammonia nitrogen (NH 4 -N), total nitrogen (TN) and total phosphorus (TP), etc. Among them, nitrogen has a variety of pollutants and is difficult to remove. When high concentrations of nitrogenous pollutants such as ammonia and nitrite are discharged into the environment, on the one hand, it will cause eutrophication of the water body, and on the other hand, it will cause eutrophication. On the one hand, it will also produce biological toxicity to a variety of organisms, so the theory and technology of denitrification have always been a researc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/16C02F101/38C12R1/05
CPCC12N1/20C02F3/34C02F2101/16C02F2101/38
Inventor 李少杰曹先贺孙宪昀张振颖胡成成
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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