Citrobacter freundii as11 and its application in sewage treatment

A technology of Freund's citric acid and AS11, which is applied in biological water/sewage treatment, water/sludge/sewage treatment, animal husbandry wastewater treatment, etc. It can solve the problems of increased investment and operation costs, limited denitrification applications, harsh growth conditions, etc. problems, to achieve the effects of saving treatment space and cost, simplifying the denitrification process, and wide pH adaptability

Active Publication Date: 2022-05-27
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biological denitrification process includes two processes of aerobic autotrophic nitrification and anaerobic heterotrophic denitrification, but it has the following disadvantages: (1) Autotrophic nitrifying bacteria grow slowly, have poor environmental adaptability, weak impact load resistance, and are easily destroyed by high Concentration of ammonia nitrogen and nitrite nitrogen inhibition
(2) The two reactions of nitrification and denitrification cannot be unified in time and space, which increases investment and operation costs
However, the suitable growth conditions of most of the strains isolated at present are relatively harsh, and the denitrification application for some special types of wastewater (such as acid-base wastewater, high-salt wastewater, and high-ammonia nitrogen wastewater) is limited. The removal of high-concentration ammonia nitrogen and the high removal rate of Citrobacter freundii (Citrobacter freundii) for swine wastewater COD and ammonia nitrogen have not been reported

Method used

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  • Citrobacter freundii as11 and its application in sewage treatment
  • Citrobacter freundii as11 and its application in sewage treatment
  • Citrobacter freundii as11 and its application in sewage treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation and identification of strains:

[0024] Enrichment culture: The sample is the activated sludge in the aerobic section collected by Cao Xianhe from a sewage treatment plant in Beijing in April 2020. Take 20 mL of the fully mixed activated sludge and put it in 180 mL of 0.2% sodium chloride solution to suspend, and take 5 mL of the activated sludge. The suspension was placed in a 250 mL Erlenmeyer flask containing 100 mL of heterotrophic nitrification medium, and subjected to acclimation and enrichment culture at 30°C and 180 rpm. Acclimation takes 48h as a cycle. After each cycle, 10 mL of enrichment solution was taken and added to a new 100 mL of heterotrophic nitrification medium to continue enrichment culture, so that continuous enrichment culture was carried out for 5 cycles. During the period, the removal of ammonia nitrogen in the culture medium was detected.

[0025] Take the culture medium after enrichment for 5 generations, use sterile water accordin...

Embodiment 2

[0036] Growth and heterotrophic nitrification properties of strain AS11:

[0037] After the strain AS11 was activated and cultured in a LB liquid test tube for 24 hours, a certain amount of bacterial liquid was drawn according to 2% of the inoculum (v / v), centrifuged at 4000 rpm for 5 minutes, and the bacterial cells were collected. In a 150 mL conical flask with 50 mL of sterilized heterotrophic nitrification medium (initial ammonia nitrogen concentration 100 mg / L, C / N=20), culture it in a shaker at 30 °C and 180 rpm for 72 h, during which sampling is performed every 4 h to measure OD600 The sample was centrifuged at 10,000 rpm for 10 min to remove the bacteria, and the supernatant was taken to detect COD and NH in the water sample.4 -N, NO 2 -N, NO 3 -N, TN content.

[0038] The results are as follows and figure 1 shown. The strain began to grow at the 8th hour after inoculation, and the strain grew rapidly after the 12th hour. At this time, NH 4 -N, TN and COD content ...

Embodiment 3

[0043] Effects of pH on growth and denitrification of strain AS11:

[0044] Adjust the pH of the heterotrophic nitrification medium to 3, 4, 5, 7, 8.5, 9.5, 10.5, and 11.5, respectively. After the strain AS11 was activated according to the conventional method, a certain amount of The bacterial liquid was centrifuged at 4000 rpm for 5 min, and the bacterial cells were collected, washed with sterile water, and then inoculated into the heterotrophic nitrification medium after adjusting the pH, and cultured in a shaker at 30 °C and 180 rpm for 72 h. The final pH conditions were detected. OD600 value, centrifuge the sample at 10,000 rpm for 10 min to remove the bacteria, and take the supernatant to detect COD and NH in the water sample. 4 -N content.

[0045] The results are as follows and figure 2 shown. Strain AS11 can grow well and perform heterotrophic nitrification in the pH range of 5-10.5. The removal rate of ammonia nitrogen is between 90% and 93%, and the removal rate ...

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Abstract

The invention belongs to the technical field of microorganisms, in particular to the technical field of microbial wastewater treatment. The invention discloses a strain of Citrobacter freundii AS11, the preservation number of which is CGMCC No.21283. The strain was deposited on December 2, 2020 in the General Microorganism Center of the China Committee for the Collection of Microbial Cultures, referred to as CGMCC, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. The strain can realize simultaneous removal of ammonia nitrogen, total nitrogen and COD under aerobic conditions.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the technical field of microbial wastewater treatment. Background technique [0002] The main pollutants that exceed the standard in my country's freshwater resources (including rivers, lakes, surface water such as large water conservancy projects, and groundwater, etc.) include chemical oxygen demand (COD), ammonia nitrogen (NH 4 -N), total nitrogen (TN) and total phosphorus (TP), etc. Among them, nitrogen has various forms of pollutants and is difficult to remove. When nitrogen-containing pollutants such as ammonia and nitrite are discharged into the environment, on the one hand, it will cause eutrophication of water bodies, and on the other hand It will also produce biological toxicity to a variety of organisms, so the theory and technology of nitrogen removal has always been a research hotspot in the field of sewage treatment. [0003] Biological nitrogen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20C02F101/16
CPCC12N1/20C02F3/34C02F2103/20C02F2101/16
Inventor 李少杰曹先贺孙宪昀张振颖胡成成
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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