Method and device for extracting bacteriocin of lactic acid bacteria through membrane separation

Abstract

The invention discloses a method and device for extracting the bacteriocin of lactic acid bacteria through membrane separation. The method comprises the following sequential steps: 1) carrying out centrifugal separation on lactic acid bacteria fermentation liquor which is fermented by a low-molecular-weight nutrient solution, the molecular weight of the raw material of the low-molecular-weight nutrient solution being not more than 4KDa; 2) performing clarification and acid water dialysis on the filtrate obtained in the step 1) by using a microfiltration membrane; (3) concentrating and washing the filtrate obtained in the step (2) by using a hydrophilic micromolecular ultrafiltration membrane; and 4) freeze-drying the concentrated solution obtained in the step 3) to obtain lactic acid bacteria bacteriocin dry powder. According to the method, the high-precision separation performance of the membrane separation technology and the hydrophilicity of the membrane surface are utilized, the low-molecular-weight nutrient solution fermentation technology is combined, and the hydrophobic lactic acid bacteria bacteriocin in the concentrated fermentation liquor is extracted, so that the active yield of the product is improved, the production process is simplified, the production flow is shortened, and the investment and operation cost is reduced; the method is very suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to a method and a device for membrane separation and extraction of bacteriocins from lactic acid bacteria, belonging to the field of extraction of bacteriocins from lactic acid bacteria. Background technique [0002] Lactobacillus bacteriocin is a product of fermentation and metabolism of Lactococcus or Lactobacillus, and it is an antibacterial peptide that has inhibitory effects on the same or different types of bacteria. Due to its safety, non-toxicity, high antibacterial activity, and no residue in degradation, some lactic acid bacteria bacteriocins have been used as food preservatives and antibacterial agents in the food and beverage industries. It can even be applied to the pharmaceutical industry as a substitute for antibiotics in the future. However, due to factors such as low yield, complicated extraction and high production costs, the commercial application of bacteriocins is restricted, and the purity of bacteriocins de...

AI Technical Summary

Problems solved by technology

The method adopts a three-stage membrane filtration method. After the mycelium and fermentation residue are removed, microfiltration and small molecule ultrafiltration are used successively to clarify and remove impurities, decolorize and concentrate, and then recrystallize and dry. This method, all The membrane separation method is used for purification and separation, and the concentration and purity are improved, but compared with the two-stage membrane filtration technology in the patent application number 200510061481.7, the process is obviously complicated, the loss of activity is increased, and the energy consumption and investment are increased.

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