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A kind of yellow croaker myosin antimicrobial peptide lcm13 and its application

A myosin and antibacterial peptide technology, applied in the biological field, can solve the problems of peptide degradation and damage to the structure of yellow croaker, and achieve the effects of improving product quality, increasing fish tenderness, pickling efficiency, and high safety.

Active Publication Date: 2022-06-28
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, high-intensity ultrasound (400-500W) has been used to assist in the pickling of yellow croakers, but the use of ultrasound at this frequency to accelerate pickling will destroy the structure of peptides in yellow croakers and degrade the peptides

Method used

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  • A kind of yellow croaker myosin antimicrobial peptide lcm13 and its application
  • A kind of yellow croaker myosin antimicrobial peptide lcm13 and its application
  • A kind of yellow croaker myosin antimicrobial peptide lcm13 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1 Yellow croaker processing technology

[0057] The processing technology is to take fresh large yellow croaker, dig out the gills, tear off the dorsal fin, and cut off the tail. Cut the knife from the back, evenly cut the fish head into two even pieces along the fish belly, without cutting the fish belly, remove the visceral dirt, wash and drain. Then lay the yellow croaker flat, sprinkle with refined salt, white wine, chives and ginger juice, put it in a sealed bag, marinate for 4 to 5 hours under the ultrasonic power of 10-40W, take it out and string it on the head of the yellow croaker with a yarn rope and tie it. Hang it in a cool and ventilated place for 3 to 4 days.

Embodiment 2

[0058] Example 2 Liquid chromatography / mass spectrometry (LCMS) of antimicrobial peptide LCM13

[0059] Weigh 100~200g of yellow croaker sample fish meat and internal organs in a beaker, add an appropriate amount of ultrapure water and crush it with a mixer for 3~5min, and then cook the crushed tissue in a 100°C water bath for 2~3h. Samples were prepared by centrifugation at 11,000 g for 20 min at 4°C. The centrifuged supernatant was then passed through a 1500-3000 mesh sieve and then frozen in a refrigerator, and then freeze-dried overnight. The lyophilized samples were then subjected to LCMS experiments.

[0060] Chromatographic conditions: Injection volume: 5.0 μl

[0061] Chromatographic column: C18 analytical chromatographic column, length 25cm, inner diameter 75μm.

[0062] Mobile phase: A: 0.1% methanol in water

[0063] B: acetonitrile

[0064] Combined with library search software: MAXQUANTv1.6.5.0 and database: uniprot large yellow croaker protein library, the m...

Embodiment 3

[0065] Example 3 Screening of antimicrobial peptide LCM13 in yellow croaker

[0066] Based on the 5 peptides obtained from LCMS results, the antibacterial sequences that may exist in the yellow croaker myosin sequence were predicted by using the antibacterial peptide prediction online server APD3, and the charge and hydrophobicity that may have antibacterial sequences were analyzed, and finally the amino acids were screened out. The sequence VSIYKLTGAVMHYGNMKFK was chemically synthesized (by Beijing Zhongke Yaguang Biotechnology Co., Ltd.), and the antibacterial activity was verified. The screening results are shown in the table below.

[0067] Table 1 Peptide analysis results of yellow croaker LCMS obtained

[0068]

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Abstract

The invention discloses a yellow croaker myosin antimicrobial peptide LCM13, the amino acid sequence of which is VSIYKLTGAVMHYGNMKFK. The molecular weight of the antimicrobial peptide is 2186Da. The invention further discloses the application of the antimicrobial peptide of yellow croaker myosin in the prevention or treatment of bacterial infections such as alginolytic Vibrio and parahaemolytic Vibrio. The invention lays a foundation for further research on the development of the yellow croaker myosin antimicrobial peptide as a food preservative and a feed additive for preventing fish diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a yellow croaker myosin antibacterial peptide LCM13 and an application thereof. Background technique [0002] In recent years, food safety problems caused by food-borne pathogenic microorganisms have become a major public health problem faced by countries around the world. According to the World Health Organization (WHO) statistics, about 1.5 billion people worldwide are infected with food-borne diseases every year, 70% of which are caused by pathogenic microbial contamination. Food-borne pathogenic microorganisms have become the main factor endangering food safety. [0003] Vibrio disease is a relatively popular and serious bacterial disease in the breeding of large yellow croaker, and its main manifestations are ulcers, rotten tail, ascites and rotten eyes. The disease has a short course, a wide range and a high mortality rate. The fatality rate was as high as 80%, and most fish...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/46A61K38/17A61P31/04A23L17/00A23L5/30A23L3/3526A23K20/147
CPCC07K14/461A61P31/04A23L17/00A23L5/32A23L3/3526A23K20/147A61K38/00
Inventor 杨燊李若冰袁紫金石林凡郑明静翁武银
Owner JIMEI UNIV
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