Fluorescence detection method of inorganic selenium

A fluorescence detection, inorganic selenium technology, applied in fluorescence/phosphorescence, measurement device, material analysis by optical means, etc., can solve the problems of high equipment cost and complicated operation, and achieve high sensitivity, less spectral interference, and accurate detection. Effect

Pending Publication Date: 2021-07-27
广州白云山维一实业股份有限公司 +1
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Problems solved by technology

[0004] The object of the present invention is to, aiming at the above-mentioned deficiencies in the prior art, provide a kind of fluorescent detection method of inorganic selenium, adopt the carbon quantum dot doped with nitrogen and boron as the fluorescent probe to realize the detection of SeO 3 2- The rapid and accurate detection of content overcomes other SeO 3 2- The detection method has problems such as high equipment cost and complicated operation

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  • Fluorescence detection method of inorganic selenium
  • Fluorescence detection method of inorganic selenium
  • Fluorescence detection method of inorganic selenium

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Embodiment 1

[0029] Embodiment 1: The fluorescent detection method of inorganic selenium provided in this embodiment uses nitrogen-boron-doped carbon quantum dots as fluorescent probes to detect SeO by fluorescence spectroscopy 3 2- content, including the following steps:

[0030] 1) Dissolve powdery nitrogen-boron co-doped carbon quantum dots (N,B-CQDs) in ultrapure water, and prepare a 0.1mg / mL D solution to test its fluorescence intensity, denoted as F 0 ;

[0031] 2) Selenite ion (SeO 3 2- ) solution is mixed with D solution respectively, obtain mixed solution respectively, carry out fluorescence test to mixed solution, obtain the fluorescence intensity value of each mixed solution respectively, denote as F;

[0032] 3) with SeO 3 2- Selenite ion concentration is the abscissa, fluorescence growth rate (F / F 0-1) is the ordinate, and linear fitting is carried out to obtain the regression equation y=kx+b, where y is the fluorescence growth rate, and x is SeO 3 2- The concentratio...

Embodiment 4

[0050] Embodiment 4: the fluorescent detection method of the inorganic selenium that this embodiment provides, it is basically the same as embodiment 1, difference is: 1) the carbon quantum dot (N, B-CQDs) that powdery nitrogen boron is co-doped Dissolved in ultrapure water, configured as 0.15mg / mL D solution, tested its fluorescence intensity, recorded as F 0 ;

[0051] 2) Selenite ion (SeO 3 2- ) solutions were mixed with D solutions to obtain mixed solutions respectively, and the mixed solutions were tested for fluorescence, and the fluorescence intensity values ​​of each mixed solutions were respectively obtained, denoted as F.

Embodiment 5

[0052] Embodiment 5: the fluorescence detection method of the inorganic selenium that this embodiment provides, it is basically the same as embodiment 1, difference is: 1) the carbon quantum dot (N, B-CQDs) that powdery nitrogen boron is co-doped Dissolved in ultrapure water, configured as 0.2mg / mL D solution, tested its fluorescence intensity, denoted as F 0 ;

[0053] 2) Selenite ion (SeO 3 2- ) solutions were mixed with D solutions to obtain mixed solutions respectively, and the mixed solutions were tested for fluorescence, and the fluorescence intensity values ​​of each mixed solutions were respectively obtained, denoted as F.

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Abstract

The invention discloses a fluorescence detection method for inorganic selenium (such as sodium selenite) containing SeO3<2->, which comprises the following steps: S1, preparing nitrogen and boron co-doped carbon quantum dot powder into a solution D, and testing the fluorescence intensity F0 of the solution D; S2, mixing SeO3<2-> solutions with different concentrations with the solution D to obtain mixed solutions, and performing fluorescence testing to obtain fluorescence intensity values F of the mixed solutions; S3, performing linear fitting by taking the concentration of SeO3<2-> as an abscissa and the fluorescence growth rate as an ordinate to obtain a regression equation y = kx + b; S4, mixing a to-be-detected solution containing SeO3<2-> with the solution D to obtain a to-be-detected sample mixed solution, performing fluorescence testing, substituting the to-be-detected sample mixed solution into a linear regression equation y = kx + b, and calculating to obtain the concentration of SeO3<2->. According to the present invention, the nitrogen and boron doped carbon quantum dot is adopted as the fluorescent probe to rapidly and accurately detect the SeO3<2-> content, and the problems of high equipment cost, complex operation and the like of other SeO3<2-> detection methods are solved.

Description

technical field [0001] The invention relates to the technical field of health product detection, in particular to a fluorescence detection method for inorganic selenium. Background technique [0002] As an essential nutrient for the human body, selenium is widely distributed in various organs, tissues, and body fluids of the human body. Usually, selenium exists in the human body in the form of antioxidant enzymes and selenoproteins, which can significantly improve immunity and enhance antioxidant capacity. Appropriate selenium concentration It is beneficial to the human body. When the selenium concentration exceeds the safe limit, the symptoms of selenium poisoning will appear, including liver cancer, tooth loss and paralysis. Selenium is mainly selenate, selenite (including SeO 3 2- ) and organic selenium exist in various forms, among which selenite is the most toxic due to excessive intake. According to research, it has been confirmed that most of the selenium poisoning ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 刘意黄明周飞燕
Owner 广州白云山维一实业股份有限公司
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