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Electrochemical immunosensing method for detecting leukemia stem cell tumor marker CD123 by multiple signal amplification technology

A technology of leukemia stem cells and tumor markers, applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., to achieve excellent clinical application value, excellent stability, economical and convenient effects

Active Publication Date: 2021-07-23
FUJIAN MEDICAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, the application of electrochemical immunosensor technology to the detection of leukemia stem cell tumor markers has not been reported yet.

Method used

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  • Electrochemical immunosensing method for detecting leukemia stem cell tumor marker CD123 by multiple signal amplification technology
  • Electrochemical immunosensing method for detecting leukemia stem cell tumor marker CD123 by multiple signal amplification technology
  • Electrochemical immunosensing method for detecting leukemia stem cell tumor marker CD123 by multiple signal amplification technology

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Embodiment Construction

[0030] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0031] Such as figure 1 As shown, firstly, the nano-gold electrode was prepared by electrodeposition, and then ATA was polymerized by cyclic voltammetry, and then K 2 S 2 o 8 and H 2 o 2 The mixed solution activates the electrode surface, enhances electron transfer, and then couples carboxyl and amino groups through EDC and NHS, and tightly connects ATA and CD123 monoclonal antibody (primary antibody), so that the monoclonal primary antibody is assembled on the surface of the gold electrode, and then reused BSA blocks the non-specific adsorption site, binds the CD123 antigen through th...

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Abstract

The invention discloses an electrochemical immunosensing method for detecting a leukemia stem cell tumor marker CD123 by a multiple signal amplification technology. The method includes the step of constructing an electrochemical immunosensing method for detecting the leukemia stem cell tumor marker CD123 by utilizing a nanogold material, an aminoterephthalic acid conductive polymer film and an enzyme catalysis multiple signal amplification technology and through detection research on the CD123. Experimental results show that under the optimal experimental condition, a good linear relation is shown between a current signal of the immunosensor and the concentration of CD123 within the concentration range of 0.02-2.5 [mu]g / mL, and the detection limit is as low as 7ng / mL. The established electrochemical immunosensor is stable, reliable, economical and convenient, has the advantages of accuracy, high sensitivity and high specificity, can be well applied to CD123 detection, provides a new detection platform for diagnosis, curative effect judgment and prognosis evaluation of leukemia patients, and has excellent potential clinical application value.

Description

technical field [0001] The invention relates to an electrochemical immunosensor method for detecting leukemia stem cell tumor marker CD123 by multiple signal amplification technology. Background technique [0002] Nanoelectrochemical immunosensor is a new type of biosensor, which combines nanotechnology, electrochemical analysis and immunology technology. It is easy to operate, low in cost, fast in analysis, high in sensitivity and good in selectivity. first choice. In recent years, electrochemical immunosensors have developed rapidly. For example, there have been many researches on electrochemical immunosensors for the detection of carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), prostate-specific antigen (PSA), CA125 and other markers. Literature reports. Nano-electrochemical immunosensors take tumor cells and their related markers as the research object, and have been widely used in clinical medicine, basic medicine, chemistry, environment, biology, agriculture,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48G01N33/574G01N33/577G01N33/58G01N33/68
CPCG01N27/3277G01N27/3278G01N27/48G01N33/57426G01N33/57488G01N33/6869G01N33/577G01N33/581G01N2333/7155Y02A50/30
Inventor 陈勇刘爱林李鑫王青
Owner FUJIAN MEDICAL UNIV
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