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Method for breeding weak post-acidification streptococcus thermophilus strain

A Streptococcus thermophilus, post-acidification technology, applied in microorganism-based methods, biochemical equipment and methods, treatment of microorganisms with electricity/wave energy, etc., can solve the problem of inability to efficiently breed weak post-acidification Streptococcus thermophilus strains problem, to achieve the effect of increasing the mutation frequency

Pending Publication Date: 2021-07-23
河北一然生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention proposes a method for breeding weakly acidified Streptococcus thermophilus strains, which solves the problem of being unable to efficiently breed weakly acidified Streptococcus thermophilus strains in the prior art

Method used

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  • Method for breeding weak post-acidification streptococcus thermophilus strain
  • Method for breeding weak post-acidification streptococcus thermophilus strain
  • Method for breeding weak post-acidification streptococcus thermophilus strain

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Preparation of bacterial suspension: Pick a single colony (starting strain) and culture it in M17 medium at 37°C for 16 hours; transfer 3% of the inoculum to fresh M17, culture it at 42°C for 4 hours, centrifuge, wash twice, concentrate (2.67 times ) in an empty plate and set aside, the M17 medium includes the following components: soybean peptone 5g, yeast extract 5g, polypeptone 5g, ascorbic acid 0.5g, beef extract 2.5g, β-glycerophosphate disodium 19g, agar 15g, magnesium sulfate 0.5ml, distilled water 1L, pH 7.0, the magnesium sulfate is 1.0mol / L magnesium sulfate aqueous solution;

[0032] (2) Ultraviolet irradiation: preheat the ultraviolet mutagenesis instrument for 30 minutes in advance, and irradiate the suspension under ultraviolet light for 5 seconds (lethal rate is 95%);

[0033] (3) Rejuvenation culture: Resuspend the bacteria in fresh M17 medium and culture at 42°C until the pH drops to 4.4;

[0034] (4) Penicillin treatment: add 10ug / mL penicillin an...

Embodiment 2

[0057] (1) Preparation of bacterial suspension: Pick a single colony (starting strain) and incubate in M17 medium at 37°C for 12 hours; transfer 3% of the inoculum to fresh M17, incubate at 42°C for 5 hours, centrifuge, wash twice, concentrate (2.67 times ) in an empty plate for subsequent use. The M17 medium includes the following components: soybean peptone 6g, yeast extract 6g, polypeptone 6g, ascorbic acid 0.8g, beef extract 2g, β-glycerophosphate disodium 18g, agar 12g , magnesium sulfate 1ml, distilled water 1L, pH 7.0, described magnesium sulfate is 1.0mol / L magnesium sulfate aqueous solution;

[0058] (2) Ultraviolet irradiation: preheat the ultraviolet mutagenesis instrument for 30 minutes in advance, and irradiate the suspension under ultraviolet light for 5 seconds (lethal rate is 95%);

[0059] (3) Rejuvenation culture: Resuspend the bacteria in fresh M17 medium and culture at 42°C until the pH drops to 4.5;

[0060] (4) Penicillin treatment: add 10ug / mL penicilli...

Embodiment 3

[0069] (1) Preparation of bacterial suspension: Pick a single colony (starting strain) and culture it in M17 medium at 37°C for 18 hours; transfer 3% of the inoculum to fresh M17, culture it at 42°C for 4.5 hours, centrifuge, wash twice, concentrate (2.67 times) in an empty plate, standby, the M17 medium includes the following components: soybean peptone 5.5g, yeast extract 5.5g, polypeptone 5g, ascorbic acid 0.6g, beef extract 2.2g, β-glycerophosphate disodium 20g, agar 14g, magnesium sulfate 0.8ml, distilled water 1L, pH 7.1, the magnesium sulfate is 1.0mol / L magnesium sulfate aqueous solution;

[0070] (2) Ultraviolet irradiation: preheat the ultraviolet mutagenesis instrument for 30 minutes in advance, and irradiate the suspension under ultraviolet light for 5 seconds (lethal rate is 95%);

[0071] (3) Rejuvenation culture: Resuspend the bacteria in fresh M17 medium and culture at 42°C until the pH drops to 4.4;

[0072] (4) Penicillin treatment: add 8ug / mL penicillin at ...

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Abstract

The invention relates to the technical field of mutation breeding of lactic acid bacteria, and provides a method for breeding weak post-acidification streptococcus thermophilus strains. The method comprises the following steps: S1, preparation of a bacterial suspension: culturing an original strain to obtain a bacterial solution, transferring and continuously culturing the bacterial solution to a logarithmic metaphase, centrifuging, washing and concentrating the bacterial solution to obtain the suspension, and placing the suspension in an empty plate for later use; S2, ultraviolet irradiation: placing the suspension under an ultraviolet lamp for irradiation; S3, rejuvenation culture: resuspending the bacteria obtained in S2 in a fresh culture medium for rejuvenation culture; S4, penicillin treatment: adding penicillin for treatment, performing dilution, and coating an MC culture medium with the solution for culture; and S5, screening weak post-acidification strains. According to the technical scheme, the problem that the weak post-acidification streptococcus thermophilus strain cannot be efficiently bred in the prior art is solved.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria mutation breeding, in particular to a method for breeding weakly acidified Streptococcus thermophilus strains. Background technique [0002] Lactic acid bacteria refers to the general term for a class of non-spore-forming, Gram-positive bacteria whose main product is lactic acid. As an important class of lactic acid bacteria, Streptococcus thermophilus is widely used in the field of fermented dairy products. Post-acidification means that the strains continue to slowly produce acid during the packaging, transportation and storage (before sales) of yogurt, resulting in an unacceptable over-sour taste for consumers, seriously affecting the quality of the product, and shortening the shelf life. It has become a key issue for fermented dairy companies. technical challenge. Contents of the invention [0003] The present invention proposes a method for breeding weakly acidified Streptococ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N13/00C12R1/46
CPCC12N15/01C12N13/00
Inventor 张彦伟杨玲仵红岩赵林森贾洪利孙策齐世华路江浩刘佳申朋
Owner 河北一然生物科技股份有限公司
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