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Supercritical cracking process of phycocyanin

A phycocyanin and supercritical technology, applied in the field of supercritical cracking process of phycocyanin, can solve the problems of low extraction efficiency, low purity, excessive solvent residue, etc., achieve quality control, no solvent residue, and solve the cost high effect

Pending Publication Date: 2021-07-16
广东普萃特医生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the technical problems of excessive solvent residue, low purity and low extraction efficiency in phycocyanin products caused by most current processes

Method used

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  • Supercritical cracking process of phycocyanin
  • Supercritical cracking process of phycocyanin

Examples

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Effect test

Embodiment 1

[0048] A supercritical cracking process of phycocyanin, comprising the following steps:

[0049] Accurately weigh 200g of spirulina powder and put it into a supercritical extraction kettle, and use a high-pressure pump to continuously introduce frozen carbon dioxide into the extraction kettle from the top at a flow rate of 15ml / min. Keep the pressure in the extraction kettle at 300bar and the temperature at 45°C; 30min Finally, collect the extract carotenoids; add 10% ethanol solution at a flow rate of 1.5ml / min, and collect the extract chlorophyll after 30 minutes;

[0050] Add 8L cracking agent methanol to the remaining spirulina powder residue in the extraction kettle, stir under normal pressure, and perform high-temperature cracking at 65°C for 16 hours to obtain a phycocyanin solution; The supercritical carbon dioxide flow is pumped in at a flow rate of 120ml / min, and the entrained solvent acetone is pumped in at a flow rate of 80mL / min. After the injection is completed, ...

Embodiment 2

[0052] A supercritical cracking process of phycocyanin, comprising the following steps:

[0053] Accurately weigh 200g of spirulina powder and put it into a supercritical extraction kettle, and use a high-pressure pump to continuously introduce frozen carbon dioxide into the extraction kettle from the top at a flow rate of 18ml / min. The pressure in the extraction kettle is maintained at 280bar and the temperature is 50°C; 30min Finally, collect the extract carotenoids; add 10% ethanol solution at a flow rate of 1.5ml / min, and collect the extract chlorophyll after 30 minutes;

[0054] The spirulina powder residue remaining in the extraction kettle was added to 5 L cracking agents methanol / chloroform, stirred at normal pressure, and subjected to high-temperature cracking at 70°C for 8 hours to obtain a phycocyanin solution; the phycocyanin solution was separated by supercritical chromatography at a flow rate of 180ml / min Pump in, the supercritical carbon dioxide flow is pumped i...

Embodiment 3

[0056] A supercritical cracking process of phycocyanin, comprising the following steps:

[0057] Accurately weigh 200g of spirulina powder and put it into a supercritical extraction kettle, and use a high-pressure pump to continuously introduce frozen carbon dioxide into the extraction kettle from the top at a flow rate of 18ml / min. Keep the pressure in the extraction kettle at 260bar and the temperature at 48°C; 30min Finally, collect the extract carotenoids; add 10% ethanol solution at a flow rate of 1.5ml / min, and collect the extract chlorophyll after 30 minutes;

[0058] Add the cracking agent 96% ethanol 12L to the spirulina powder residue remaining in the extraction kettle, stir under pressure at 0.2Mpa, and perform high-temperature cracking at 120°C for 1 hour to obtain a phycocyanin solution; the phycocyanin solution is separated by supercritical chromatography at 220ml / min The flow rate is pumped in, the supercritical carbon dioxide flow is pumped in at a flow rate of...

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Abstract

A supercritical cracking process of phycocyanin is provided. The supercritical cracking process comprises the following steps: S1, loading dried spirulina powder into a supercritical extraction kettle, continuously introducing carbon dioxide into the extraction kettle at a constant flow rate, keeping proper pressure and temperature in the extraction kettle, and collecting an extract that is carotenoid, adding an ethanol solution, and collecting an extract that is chlorophyll; S2, adding a cracking agent into residual spirulina powder residues in the extraction kettle, and performing high-temperature cracking to obtain a phycocyanin solution; and S3, carrying out supercritical chromatographic separation on the phycocyanin solution, pumping the phycocyanin solution at a certain sample injection flow rate, pumping the phycocyanin solution at a certain sample injection flow rate in a manner that the supercritical carbon dioxide fluid carries the solvent, and eluting residual solvent impurities in the phycocyanin to obtain the phycocyanin. According to the method, the phycocyanin is accurately extracted and separated, the effects of complete elution and zero solvent residue can be achieved, the quality is controllable, and the safe edible performance of the phycocyanobilin product is guaranteed.

Description

technical field [0001] The invention relates to the technical field of supercritical cracking, in particular to a supercritical cracking process of phycocyanin. Background technique [0002] Spirulina, also known as Arthrospira or cyanobacteria, belongs to the phylum Cyanophyta, mainly including Spirulina maxima, Spirulina pureatops and Spirulina spirulina. Spirulina is extremely rich in nutrients and contains rich vitamins and trace elements needed by the human body. It can be used as a high-protein, low-fat, low-cholesterol, and low-calorie health food. It is recommended by the United Nations Food and Agriculture Organization as "the most ideal human tomorrow" food". It is rich in protein and a variety of pigments, especially phycocyanin, which is a nutritional pigment that is rarely seen in natural food coloring varieties. [0003] Phycocyanin is extracted from algae cells containing phycobilisomes. It is the only light-harvesting pigment protein in cyanobacteria. It ha...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096Y02P20/54
Inventor 胡勇刚李桂珠李伟明
Owner 广东普萃特医生物工程有限公司
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