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Autologous collagen injection combined with clostridium botulinum

A technology of collagen and botulinum toxin, which is applied in the field of medical cosmetology, can solve the problems of being easily degraded by the body and not fundamentally solving the problem of wrinkles, so as to reduce the rejection of the human body, improve the effect of wrinkle removal, and ensure tissue compatibility. sexual effect

Inactive Publication Date: 2021-07-16
白晋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Removing wrinkles through autologous collagen injection can significantly reduce allergic reactions, but simple collagen injection is easily degraded by the body and cannot fundamentally solve the problem of wrinkles. Therefore, the existing medical collagen injection fillers are improved. It is of great significance to improve the effect of removing wrinkles

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The embodiment of the present invention provides a preparation method of autologous collagen injection combined with botulinum toxin, comprising the following steps:

[0021] Step 1: Obtain the autologous fibroblast tissue, wash it with normal saline at room temperature, cut the autologous fibroblast tissue into small pieces, add phosphate buffered saline solution to the mixture, and centrifuge at room temperature to remove the lower liquid at a centrifugation speed of 1200rpm-3500rpm. The centrifugation time is 2min to 5min. After washing with phosphate buffered saline solution, centrifuge again, remove the supernatant, add collagenase, place in a constant temperature shaking box at 37°C for 30min to 60min, centrifuge again to remove the supernatant, and then add serum content of 15 % fetal bovine serum DMEM culture solution, filtered through a cell sieve and centrifuged to remove the supernatant, then added to the DMEM culture solution containing fetal bovine serum aga...

Embodiment 1

[0031] Autologous collagen injections combined with Botox are prepared using the following steps:

[0032]Step 1: Obtain the autologous subcutaneous fibroblast tissue, wash it with normal saline at room temperature, cut the autologous fibroblast tissue into small pieces, add phosphate buffered saline solution to it, and centrifuge at room temperature to remove the lower liquid. The time is 5 minutes, washed with phosphate buffered saline solution, centrifuged again, removed the supernatant, added collagenase, placed in a constant temperature shaking box at 37°C for 60 minutes, centrifuged again to remove the supernatant, and then added DMEM culture medium containing fetal bovine serum , filtered through a cell sieve, centrifuged to remove the supernatant, and added to the DMEM medium containing fetal bovine serum again, at 37 ° C, 5% CO 2 Constant temperature culture;

[0033] Step 2, subculture, the fibroblasts in step 1 are replaced with the DMEM culture medium of fetal bov...

Embodiment 2

[0037] Autologous collagen injections combined with Botox are prepared using the following steps:

[0038] Step 1: Obtain the autologous subcutaneous fibroblast tissue, wash it with normal saline at room temperature, cut the autologous fibroblast tissue into small pieces, add phosphate buffered saline solution to it, and centrifuge at room temperature to remove the lower liquid. The time is 2 minutes, washed with phosphate buffered saline solution and then centrifuged again to remove the supernatant, add collagenase, place in a constant temperature shaking box at 37°C for 60 minutes, centrifuge again to remove the supernatant, and then add DMEM culture solution containing fetal bovine serum , filtered through a cell sieve, centrifuged to remove the supernatant, and added to the DMEM medium containing fetal bovine serum again, at 37 ° C, 5% CO 2 Constant temperature culture;

[0039] Step 2, subculture, the fibroblasts in step 1 are replaced with the DMEM culture medium of fet...

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Abstract

The invention relates to an autologous collagen injection combined with clostridium botulinum, and belongs to the technical field of medical cosmetology. The autologous collagen injection is prepared from the following raw materials of fibroblasts, collagen, a Ringer's solution I, a glucose solution and the clostridium botulinum, wherein the mass volume ratio of the fibroblasts to the collagen to the Ringer's solution I to the glucose solution to the clostridium botulinum is 10g: (5ml to 20ml): (10ml to 30ml): (5 mcg to 20 mcg). According to the technical scheme, autologous fibroblasts are separated, the collagen is secreted through the fibroblasts, rejection reaction of a human body can be reduced after the collagen is used as the autologous collagen injection, the clostridium botulinum is combined with the autologous collagen injection, muscles of wrinkles caused by the clostridium botulinum are paralyzed, the muscles are not shrunk, and meanwhile, the fibroblasts and other active ingredients in the injection are utilized, so that the tissue compatibility in a body is ensured, and the wrinkle removing effect is improved.

Description

[0001] This application is a divisional application of the Chinese patent application with the application number "201810930860.3" and the application name "A method for preparing autologous collagen injection combined with botulinum toxin". The application date of the parent application is 2018.08.15. technical field [0002] The invention relates to a preparation method of an autologous collagen injection combined with botulinum toxin, belonging to the technical field of medical cosmetology. Background technique [0003] Wrinkles refer to the small fine lines and wrinkles formed by the skin being affected by the external environment to form free radicals, free radicals destroy collagen and active substances in normal cell membrane tissues, and oxidize cells. There are four reasons for the occurrence of wrinkles: natural aging, gravity, ultraviolet radiation in the sun, which causes photoaging and photodamage of the skin and excessive contraction of facial expression muscles...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/24A61L27/50A61L27/54
CPCA61L27/24A61L27/3637A61L27/3804A61L27/3895A61L27/50A61L27/54A61L2300/414A61L2400/06
Inventor 白晋
Owner 白晋
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