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Wound dressing capable of releasing nitric oxide

A wound dressing and nitric oxide technology, applied in the field of medical materials, to achieve strong adaptability, avoid easy degradation of raw materials, and reduce storage requirements

Pending Publication Date: 2021-07-13
SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the research technology, NO precursor compounds such as O-nitroso compounds, S-nitroso compounds, nonanoic acid compounds, inorganic nitroso compounds, stiltonimines, etc. are used to make them react under suitable chemical conditions. NO can be decomposed under lower conditions, but the local chemical conditions of the wound are not always suitable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] A method for preparing a first dressing with a dry material as a carrier, comprising: preparing a first mixed solution containing NO synthase and additives, soaking the washed and dried dry material in the first mixed solution, or adding the first A mixed solution is sprayed on the washed and dried dry material, and the first dressing is obtained after drying.

[0035] Wherein, in the first mixed solution, when the additive is double-stranded DNA and chitosan, the preferred concentration of DNA in the mixed solution is 0.01-0.05 mg / mL, and the preferred concentration of chitosan is 0.01-0.05 mg / mL; when the additive is When poly-L-lysine / polyethylene glycol block copolymer, its preferred concentration is 1-3mg / mL; when the additive is sodium carboxymethyl cellulose, its preferred concentration is 0.2-0.8wt%;

[0036] When eNOS or iNOS is used as NO synthase, the preferred concentration is 200-500 ng / mL.

[0037] Moreover, the preferred preparation method of the first m...

Embodiment 1

[0046] A kind of polyurethane foam dressing bearing eNOS, its preparation method is as follows:

[0047] 1) Fully clean and dry the polyurethane foam;

[0048] 2) Mix double-stranded DNA with chitosan solution to obtain DNA / chitosan solution, then add eNOS recombinant protein solution to the solution and mix evenly to obtain a solution containing 0.02mg / mL DNA, 0.02mg / mL chitosan , 200ng / mL eNOS dipping solution;

[0049] 3) Soak the polyurethane foam in the dipping solution at 4°C for 30 minutes, so that the DNA / chitosan containing eNOS is fully dip-coated on the polyurethane foam;

[0050] 4) The polyurethane foam was taken out from the solution and air-dried to obtain a polyurethane foam dressing loaded with eNOS, wherein the eNOS load was 500 ng / g, and the dressing was stored in a dry environment at 4°C.

[0051] Similarly, in this example, cotton yarn was used as the dressing matrix, and the above preparation process was repeated to obtain a cotton yarn dressing carryin...

Embodiment 2

[0054] A kind of polyurethane foam dressing that carries iNOS, its preparation method is as follows:

[0055] 1) Fully clean and dry the polyurethane foam;

[0056] 2) Dissolve the poly-L-lysine / polyethylene glycol block copolymer in phosphate buffered saline (0.02mol / L, pH 7.4) at room temperature to obtain a mixed solution, and then add the mixed solution dropwise into the iNOS recombinant protein solution and mixed uniformly to obtain a dipping solution containing 2mg / mL poly-L-lysine / polyethylene glycol block copolymer and 1000ng / mL iNOS;

[0057] 3) Soak the polyurethane foam in the dipping solution at 4°C for 30 minutes, so that the poly-L-lysine / polyethylene glycol block copolymer containing iNOS is fully dip-coated on the polyurethane foam;

[0058] 4) The polyurethane foam was taken out from the solution and air-dried to obtain a polyurethane foam dressing carrying iNOS, and the dressing was stored at 4°C.

[0059] Similarly, in this example, cotton yarn was used as...

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PUM

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Abstract

The invention relates to a wound dressing capable of releasing nitric oxide. The wound dressing comprises a first dressing containing NO synthase and a second dressing containing a substrate capable of interacting with the NO synthase, during storage, the NO synthase is isolated from the substrate; when the dressing is used, the first dressing and the second dressing are stacked and cover the surface of the wound, the NO synthase on the first dressing and the substrate on the second dressing are mixed through outflow of wound seepage, and NO is generated and acts on wound repair. Compared with the prior art, the method has the advantages that NO is generated by establishing a physiological system which is the same as that in an organism, the dressing has higher adaptability to physical, chemical and physiological conditions of the wound environment, and the dressing has the advantages of being adjustable in NO generation rate, convenient to store and the like.

Description

technical field [0001] The invention belongs to the technical field of medical materials and relates to a wound dressing capable of releasing nitric oxide. Background technique [0002] Nitric oxide (NO) is a very important biological messenger involved in the regulation of many important physiological activities in the body. It is a potent vasodilator that increases local blood flow and also promotes angiogenesis in conjunction with other growth factors. It also has the effect of killing pathogenic microorganisms and reducing inflammation. NO plays a very important role in wound repair. It participates in regulating multiple aspects of wound repair, such as improving collagen production, promoting blood vessel growth, regulating inflammatory responses, and promoting the differentiation and reproduction of various skin cells. Studies have also shown that low NO levels on the wound surface delay wound repair, and the wound closure time of mice whose NO synthase (NOS) was re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F13/02A61H33/14A61M35/00
CPCA61F13/02A61F13/0289A61F2013/0017A61F2013/00906A61H33/14A61H2033/146A61M35/10A61M35/30
Inventor 黄钢尹华清
Owner SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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