Wound dressing capable of releasing nitric oxide
A wound dressing and nitric oxide technology, applied in the field of medical materials, to achieve strong adaptability, avoid easy degradation of raw materials, and reduce storage requirements
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preparation example Construction
[0034] A method for preparing a first dressing with a dry material as a carrier, comprising: preparing a first mixed solution containing NO synthase and additives, soaking the washed and dried dry material in the first mixed solution, or adding the first A mixed solution is sprayed on the washed and dried dry material, and the first dressing is obtained after drying.
[0035] Wherein, in the first mixed solution, when the additive is double-stranded DNA and chitosan, the preferred concentration of DNA in the mixed solution is 0.01-0.05 mg / mL, and the preferred concentration of chitosan is 0.01-0.05 mg / mL; when the additive is When poly-L-lysine / polyethylene glycol block copolymer, its preferred concentration is 1-3mg / mL; when the additive is sodium carboxymethyl cellulose, its preferred concentration is 0.2-0.8wt%;
[0036] When eNOS or iNOS is used as NO synthase, the preferred concentration is 200-500 ng / mL.
[0037] Moreover, the preferred preparation method of the first m...
Embodiment 1
[0046] A kind of polyurethane foam dressing bearing eNOS, its preparation method is as follows:
[0047] 1) Fully clean and dry the polyurethane foam;
[0048] 2) Mix double-stranded DNA with chitosan solution to obtain DNA / chitosan solution, then add eNOS recombinant protein solution to the solution and mix evenly to obtain a solution containing 0.02mg / mL DNA, 0.02mg / mL chitosan , 200ng / mL eNOS dipping solution;
[0049] 3) Soak the polyurethane foam in the dipping solution at 4°C for 30 minutes, so that the DNA / chitosan containing eNOS is fully dip-coated on the polyurethane foam;
[0050] 4) The polyurethane foam was taken out from the solution and air-dried to obtain a polyurethane foam dressing loaded with eNOS, wherein the eNOS load was 500 ng / g, and the dressing was stored in a dry environment at 4°C.
[0051] Similarly, in this example, cotton yarn was used as the dressing matrix, and the above preparation process was repeated to obtain a cotton yarn dressing carryin...
Embodiment 2
[0054] A kind of polyurethane foam dressing that carries iNOS, its preparation method is as follows:
[0055] 1) Fully clean and dry the polyurethane foam;
[0056] 2) Dissolve the poly-L-lysine / polyethylene glycol block copolymer in phosphate buffered saline (0.02mol / L, pH 7.4) at room temperature to obtain a mixed solution, and then add the mixed solution dropwise into the iNOS recombinant protein solution and mixed uniformly to obtain a dipping solution containing 2mg / mL poly-L-lysine / polyethylene glycol block copolymer and 1000ng / mL iNOS;
[0057] 3) Soak the polyurethane foam in the dipping solution at 4°C for 30 minutes, so that the poly-L-lysine / polyethylene glycol block copolymer containing iNOS is fully dip-coated on the polyurethane foam;
[0058] 4) The polyurethane foam was taken out from the solution and air-dried to obtain a polyurethane foam dressing carrying iNOS, and the dressing was stored at 4°C.
[0059] Similarly, in this example, cotton yarn was used as...
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