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Application of anise plant or extract thereof in preparation of anti-animal virus medicine

A technology of plant extracts and animal viruses, applied in antiviral agents, plant raw materials, plant/algae/fungus/moss components, etc.

Active Publication Date: 2021-07-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this activity is an inhibitory effect on the proliferation of liver cancer cells, not a real antiviral effect

Method used

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  • Application of anise plant or extract thereof in preparation of anti-animal virus medicine
  • Application of anise plant or extract thereof in preparation of anti-animal virus medicine
  • Application of anise plant or extract thereof in preparation of anti-animal virus medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: preparation of star anise plant extract

[0058] Take medicinal materials, pulverize them, put them in a round bottom flask, and add 75% ethanol to reflux for extraction. For the first time, 10-12 times of 75% ethanol was refluxed for 2 hours; for the second time, 8-10 times of 75% ethanol was extracted for 2 hours. The extract was filtered, and the filtrates were combined. Use a rotary evaporator to reclaim ethanol under reduced pressure in a 45°C water bath until there is no alcohol smell, and continue to concentrate and freeze-dry to obtain the ethanol extract EEIL (yield 21.51%) and ethanol alcohol extract EEIH (yield 9.16%) ) and EEIJ (yield 13.78%)

Embodiment 2

[0059] Embodiment 2: preparation of red fennel injection

[0060] Get the ethanol extract (equivalent to 50g of the original drug), add 800ml water for injection and 20ml polysorbate 80, stir well, adjust the pH value to about 9.7 with 10% sodium hydroxide solution, add water for injection to 1000ml, Stir well, filter, pot, and sterilize to obtain red fennel injection (HHXI). Each ml contains 50mg of the original drug of red poisonous fennel.

Embodiment 3

[0061] Embodiment 3: In vitro to the killing effect of pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV)

[0062] Taking the maximum safe concentration (maximum no-cytotoxic concentration, MNTC) for African green monkey kidney Vero cells and African green monkey embryonic kidney Marc-145 cells as the initial concentration, after doubling dilution with DMEM culture medium, the drug dilution with 200TCID 50 Mix equal volumes of PRV or PRRSV virus dilutions, and store at 37°C, 5% CO 2 Incubate for 2 hours in the incubator. Add 100 μL per well to a 96-well plate that has grown into a monolayer of Vero or Marc-145 cells after 24 hours of adherent culture, with 8 replicate wells. At the same time, set blank control, normal cell control and virus control. At 37°C, 5% CO 2 Culture in an incubator with shaking every 30 minutes. After incubation for 2 hours, discard the virus and drug mixture in the wells, add 100 μL of maintenance solution t...

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Abstract

The invention relates to an application of an anise plant or an extract of the anise plant in preparation of an anti-animal virus medicine. The anise plant is I.lanceolatum A.C. Smith, I.henryi Diels or Illicium jiadifengpi B.N.Chang. The anise plant extract disclosed by the invention can effectively and directly kill animal viruses in vitro, prevent the animal viruses from entering cells and inhibit the animal viruses from being copied in the cells to play an antiviral role. Meanwhile, the apoptosis of the host cells induced by animal viruses can be inhibited, and the inflammatory reaction of the host cells induced by the animal viruses is inhibited through NF-kappa B and MAPKs pathways, so that the host cells are protected. The product has remarkable prevention and treatment effects on mice infected by animal viruses, and can remarkably reduce the virus load of brain tissues of the mice, reduce the expression level of inflammatory factor genes, inhibit cytokine storm, relieve pathological changes of the brain tissues, improve the survival rate of the mice infected by the animal viruses and prolong the survival time of the mice infected by the animal viruses.

Description

technical field [0001] Anti-animal virus effects and uses of plants of the genus Illicium and their extracts, characterized in that the plants of the genus Illicium include Illicium lanceolatum A.C.Smith, red fennel (I.henryi Diels) or False maple bark ( I. jiadifengpi B.N. Chang). Background technique [0002] With the intensification and large-scale development of livestock and poultry, the illegal use and abuse of vaccines and antibiotics in the breeding process will cause mutations in pathogens, the body is in a sub-healthy state, immune function is reduced, disease resistance is reduced, and new and re-emerged infectious diseases The outbreak and prevalence of the disease not only severely restrict the healthy development of the breeding industry, but also the cross-species transmission of some zoonotic disease pathogens poses a huge challenge to global public health. [0003] At present, comprehensive prevention and control measures of immune prevention and drug preve...

Claims

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Application Information

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IPC IPC(8): A61K36/57A61P31/12A61P31/22A61P31/14
CPCA61K36/57A61P31/12A61P31/22A61P31/14A61K2236/331A61K2236/33A61K2236/333A61K2236/39A61K2236/51
Inventor 孙红祥苗灵燕何燕飞
Owner ZHEJIANG UNIV
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