Rapid propagation and standardized cultivation method for radix angelicae pubescentis

A cultivation method and solitary living technology, applied in the field of plant cultivation, can solve the problems that the invasion of diseases and insect pests and pesticide residues cannot be ruled out, the cost of solitary living planting is high, and the consumption of nutrients, etc., so as to eliminate the invasion of diseases and insect pests and pesticide residues, and reduce the cost of solitary planting , the effect of promoting cell proliferation speed

Inactive Publication Date: 2021-07-09
张家界常满种植有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, due to the sharp increase in market demand, the price of solitary live has continued to rise, resulting in the predatory collection of wild solitary live. Coupled with the influence of its own internal factors, the growth area of ​​wild solitary live has been greatly reduced. In order to meet market demand, the planting area of ​​solitary live is expanded. , increase the production level, increase economic benefits, the area of ​​artificial cultivation of solitary live has increased significantly, and the seedlings are in short supply, resulting in high prices for solitary live products, resulting in increased costs for pharmaceutical companies, increased expenses, and soaring prices for finished drugs. There are deficiencies, as follows:
[0005] 1) Cell proliferation speed cannot be promoted under the growth conditions of lack of water and fertilizer, and the nutrients in the plant cannot be consumed, and the flowering conditions of solitary liveness are destroyed, the proliferation speed is reduced, the production cycle is long, the equipment is complicated, and the floor area is large. It cannot save manpower, material resources, etc., and is not convenient for factory production;
[0006] 2) Slow germination, low multiplication rate, complex methods, high production costs, no mass production, low application value, and restricted by factors such as seasons, climatic conditions and soil, the invasion of diseases and insect pests and the impact of pesticide residues cannot be ruled out. quality of solitude;
[0007] 3) It is necessary to plant solitary seedlings in addition, the cost of solitary planting is high, and its economic benefits are low, and the use of solitary seedlings for artificial cultivation results in large individual differences and uneven quality. Live wild resources alone, increasing the destruction of natural resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Materials: solitary living roots are used as explants, and the specific steps are as follows:

[0048] Step 1, selection and sterilization of explants: select the living root as the culture material, wash it with tap water, rinse it under running water for 12 minutes, place it in 75% alcohol for 5-10 seconds, and then treat it with 0.1% alcohol Mercurate disinfection for 8 minutes, rinse with sterile water and set aside;

[0049] Step 2, germination of sprouts: inoculate the sterilized sprouts on MS+6-BA0.8 mg / L+NAA0.3mg / L medium, the pH value is 6.2, and the culture temperature is 25-28°C. 10 hours of light per day, light intensity 1500LX, buds germinate 8-12 days after inoculation, and seedlings with leaves form in about 15 days;

[0050] Step 3, cluster bud induction and proliferation culture: Cut off the seedlings and transfer to MS+6-BA0.6mg / L+IAA0.5mg / L medium until cluster buds are formed, the pH value is 6.2, and the culture temperature is 25 -28°C, 13 hours o...

Embodiment 2

[0060] Materials: solitary living roots are used as explants, and the specific steps are as follows:

[0061] Step 1, selection and sterilization of explants: select the living root as the culture material, wash it with tap water, rinse it under running water for 12 minutes, place it in 75% alcohol for 5-10 seconds, and then treat it with 0.1% alcohol Mercurate disinfection for 6 minutes, rinse with sterile water and set aside;

[0062] Step 2, germination of sprouts: inoculate the sterilized sprouts on MS+6-BA0.8 mg / L+NAA0.3mg / L medium, the pH value is 6.2, and the culture temperature is 25-28°C. 10 hours of light per day, light intensity 1500LX, buds germinate 8-12 days after inoculation, and seedlings with leaves form in about 15 days;

[0063] Step 3, cluster bud induction and proliferation culture: Cut off the seedlings and transfer to MS+6-BA0.6mg / L+IAA0.5mg / L medium until cluster buds are formed, the pH value is 6.2, and the culture temperature is 25 -28°C, 13 hours o...

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Abstract

The invention relates to the technical field of plant cultivation, in particular to a rapid propagation and standardized cultivation method for radix angelicae pubescentis. The cultivated radix angelicae pubescentis is rapid in budding, high in proliferation rate, simple in method, low in production cost, capable of being produced in batches and high in application value, the production of the radix angelicae pubescentis can be carried out under manual control conditions and is not limited by factors such as seasons, climate conditions and soil, the influence of invasion of diseases and insect pests and pesticide residues can be eliminated, the quality of the radix angelicae pubescentis is strictly controlled, radix angelicae pubescentis seedlings do not need to be additionally planted through the design, the planting cost of the radix angelicae pubescentis is greatly reduced, and the economic benefits are remarkably improved; and radix angelicae pubescentis seedlings obtained by using the tissue culture rapid propagation technology are used for artificial cultivation, finished radix angelicae pubescentis with small individual difference and uniform quality can be obtained, germplasm resources of the radix angelicae pubescentis can be preserved, wild resources of the radix angelicae pubescentis can be protected, and damage to natural resources is reduced.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a solitary living rapid propagation and standardized cultivation method. Background technique [0002] my country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in my country and corresponding countries and regions. The traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources. In order to solve the contradiction between supply and demand of medicinal plants, people use artificial cultivation to expand the source of medicine. However, among the artificially cultivated medicinal plants, there are many precious medicinal materials with a long production cycle, such as ginseng, magnolia officinalis, Phellodendron cork, etc. It takes a long time to breed or raise seedlings by conventional methods; Plants such as Fritillaria, crocus, etc., ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G22/00A01G13/00A01C21/00
CPCA01H4/008A01H4/001A01G22/00A01G13/00A01C21/005
Inventor 朱卫东
Owner 张家界常满种植有限公司
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